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Plant Molecular Biology

, Volume 57, Issue 4, pp 517–540 | Cite as

Systemic response to aphid infestation by Myzus persicae in the phloem of Apium graveolens

  • Fanchon Divol
  • Françoise Vilaine
  • Sandra Thibivilliers
  • Joëlle Amselem
  • Jean-Christophe Palauqui
  • Chantal Kusiak
  • Sylvie DinantEmail author
Article

Abstract

Little is known about the molecular processes involved in the phloem response to aphid feeding. We investigated molecular responses to aphid feeding on celery (Apium graveolenscv. Dulce) plants infested with the aphid Myzus persicae, as a means of identifying changes in phloem function. We used celery as our model species as it is easy to separate the phloem from the surrounding tissues in the petioles of mature leaves of this species. We generated a total of 1187 expressed sequence tags (ESTs), corresponding to 891 non-redundant genes. We analysed these ESTs in silico after cDNA macroarray hybridisation. Aphid feeding led to significant increase in RNA accumulation for 126 different genes. Different patterns of deregulation were observed, including transitory or stable induction 3 or 7 days after infestation. The genes affected belonged to various functional categories and were induced systemically in the phloem after infestation. In particular, genes involved in cell wall modification, water transport, vitamin biosynthesis, photosynthesis, carbon assimilation and nitrogen and carbon mobilisation were up-regulated in the phloem. Further analysis of the response in the phloem or xylem suggested that a component of the response was developed more specifically in the phloem. However, this component was different from the stress responses in the phloem driven by pathogen infection. Our results indicate that the phloem is actively involved in multiple adjustments, recruiting metabolic pathways and in structural changes far from aphid feeding sites. However, they also suggest that the phloem displays specific mechanisms that may not be induced in other tissues.

Keywords

Compatible interaction EST Gene expression Plant defence Plant–insect interaction Transcriptome 

Abbreviations

CMV

Cucumber mosaic cucumovirus

EST

Expressed sequence tag

JA

Jasmonic acid

PR

Pathogenesis-related

SA

Salicylic acid

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Copyright information

© Springer 2005

Authors and Affiliations

  • Fanchon Divol
    • 1
  • Françoise Vilaine
    • 1
  • Sandra Thibivilliers
    • 1
  • Joëlle Amselem
    • 1
  • Jean-Christophe Palauqui
    • 1
  • Chantal Kusiak
    • 1
  • Sylvie Dinant
    • 1
    Email author
  1. 1.Laboratory of Cell BiologyINRAVersaillesFrance

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