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A novel glucosyltransferase involved in steroid saponin biosynthesis in Solanum aculeatissimum

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Abstract

Steroidal saponins are widely distributed in many plant species. Their diverse structures have resulted in a wide range of applications, including drug and medicine production. It has been suggested that the nature of the non-saccharide and oligosaccharide portions of the saponin molecule both contribute to the properties of individual saponins. Despite numerous studies on the occurrence, chemical structure, and varying pharmaceutical activities of steroidal saponins, their biosynthesis pathway is poorly understood. Glycosylation is thought to be the final step in steroidal saponin biosynthesis and it is thought to be involved in regulating the biological activities of saponins. Isolation of the glycosyltransferases that catalyze the transfer of sugar molecules to steroidal compounds will help to clarify the mechanisms that produce diverse saponins and control their activities in plants. In this study, we obtained three cDNAs encoding putative glycosyltransferases from Solanum aculeatissimum. One of the three, SaGT4A showed UDP-glucosyltransferase activity. This is the first cloned glucosyltransferase involved in steroidal saponin biosynthesis. SaGT4A catalyzes the 3-O-glucosylation of steroidal sapogenins, such as diosgenin, nuatigenin, and tigogenin. This enzyme also glucosylates steroidal alkaloids, such as solanidine, solasodine, and tomatidine. Gene expression analysis revealed that the accumulation of SaGT4A transcripts showed a unique response to wounding stress indicating the involvement of SaGT4A in plant defense system.

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Abbreviations

GT:

glucosyltransferase

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Authors and Affiliations

  1. Plant Science Center, RIKEN, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan

    Atsuko Kohara, Chiharu Nakajima, Kimiko Hashimoto, Shigeo Yoshida & Toshiya Muranaka

  2. Graduate School of Sciences and Technology, Nagasaki University, 1–14 Bunkyo-machi, Nagasaki, 852-8521, Japan

    Toshihiko Ikenaga

  3. Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1, Maidashi, Fukuoka, Higashi-ku, 812-8582, Japan

    Hiroyuki Tanaka & Yukihiro Shoyama

  4. Kyoto Pharmaceutical University, 1 Shichono-cho, Misasagi, Yamashina-ku, Kyoto, 607-8412, Japan

    Kimiko Hashimoto

Authors
  1. Atsuko Kohara
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  2. Chiharu Nakajima
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  3. Kimiko Hashimoto
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  4. Toshihiko Ikenaga
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  5. Hiroyuki Tanaka
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  6. Yukihiro Shoyama
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  7. Shigeo Yoshida
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  8. Toshiya Muranaka
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Corresponding author

Correspondence to Toshiya Muranaka.

Additional information

This work was supported in part by a Grant-in-Aid (No. 15510189) for Scientific Research (C) to T.M. from the Japan Society for the Promotion of Science.

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Kohara, A., Nakajima, C., Hashimoto, K. et al. A novel glucosyltransferase involved in steroid saponin biosynthesis in Solanum aculeatissimum. Plant Mol Biol 57, 225–239 (2005). https://doi.org/10.1007/s11103-004-7204-2

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  • DOI: https://doi.org/10.1007/s11103-004-7204-2

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