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Analysis of the S-locus structure in Prunus armeniaca L. Identification ofS-haplotype specific S-RNase and F-box genes

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Abstract

The gametophytic self-incompatibility (GSI) system in Rosaceae has been proposed to be controlled by two genes located in the S-locusan S-RNase and a recently described pollen expressed S-haplotype specific F-box gene (SFB). However, in apricot (Prunus armeniaca L.) these genes had not been identified yet. We have sequenced 21kb in total of the S-locus region in 3 different apricot S-haplotypes. These fragments contain genes homologous to the S-RNase and F-box genes found in other Prunusspecies, preserving their basic gene structure features and defined amino acid domains. The physical distance between the F-boxand the S-RNase genes was determined exactly in the S 2-haplotype (2.9kb) and inferred approximately in the S 1-haplotype (< 49kb) confirming that these genes are linked. Sequence analysis of the 5′ flanking regions indicates the presence of a conserved region upstream of the putative TATA box in the S-RNase gene. The three identified S-RNase alleles (S 1, S 2 and S 4) had a high allelic sequence diversity (75.3 amino acid identity), and the apricot F-box allelic variants (SFB1, SFB2 and SFB4) were also highly haplotype-specific (79.4 amino acid identity). Organ specific-expression was also studied, revealing that S 1- and S 2-RNases are expressed in style tissues, but not in pollen or leaves. In contrast, SFB 1 and SFB 2 are only expressed in pollen, but not in styles or leaves. Taken together, these results support these genes as candidates for the pistil and pollen S-determinants of GSI in apricot.

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Authors and Affiliations

  1. Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera Km 5,5, Moncada (Valencia), 46113, Spain

    C. Romero, S. Vilanova, J. Martínez-Calvo, M. Vicente, G. Llácer & M. L. Badenes

  2. Departamento de Mejora Genética y Patología Vegetal, CEBAS-CSIC, Apartado 4195, Murcia, 30080, Spain

    L. Burgos

  3. Departamento de Genética, Universidad de Valencia, Campus de Burjassot C/ Dr. Moliner 50, Burjassot (Valencia), 46100, Spain

    M. Vicente

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  1. C. Romero
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  2. S. Vilanova
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  3. L. Burgos
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  4. J. Martínez-Calvo
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Romero, C., Vilanova, S., Burgos, L. et al. Analysis of the S-locus structure in Prunus armeniaca L. Identification ofS-haplotype specific S-RNase and F-box genes. Plant Mol Biol 56, 145–157 (2004). https://doi.org/10.1007/s11103-004-2651-3

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