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Identification of more than 200 glucose-responsive Arabidopsis genes none of which responds to 3-O-methylglucose or 6-deoxyglucose

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Abstract

The response of some plant genes to glucose analogues 3-O-methylglucose (3OMG) or 6-deoxyglucose (6DOG) has been cited as evidence for metabolism-independent glucose signalling. To analyse such signalling using a genetic approach, we sought to identify Arabidopsis glucose-responsive genes which also respond to 3OMG and 6DOG in seedlings. Microarray analysis of gene expression in glucose-treated seedlings and RT-PCR analysis of glucose-treated leaf sections identified more than 200 glucose-responsive genes, but none responded to 3OMG or 6DOG. These data together with other published data on individual genes fail to identify any Arabidopsis sugar-responsive genes which also respond to 3OMG or 6DOG.

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  1. Institute of Cell and Molecular Biology, University of Edinburgh, The King's Buildings, Mayfield Road, EH9 3JH, UK

    Dorthe Villadsen & Steven Smith

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  1. Dorthe Villadsen
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  2. Steven Smith
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Villadsen, D., Smith, S. Identification of more than 200 glucose-responsive Arabidopsis genes none of which responds to 3-O-methylglucose or 6-deoxyglucose. Plant Mol Biol 55, 467–477 (2004). https://doi.org/10.1007/s11103-004-1050-0

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