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A unigene catalogue of 5700 expressed genes in cassava

Plant Molecular Biology volume 56, pages 541–554 (2004)Cite this article

Abstract

Two economically important characters, starch content and cassava bacterial blight resistance, were targeted to generate a large collection of cassava ESTs. Two libraries were constructed from cassava root tissues of varieties with high and low starch contents. Other libraries were constructed from plant tissues challenged by the pathogen Xanthomonas axonopodis pv.manihotis. We report here the single pass sequencing of 11 954 cDNA clones from the 5’ ends, including 111 from the 3’ ends. Cluster analysis permitted the identification of a unigene set of 5700 sequences. Sequence analyses permitted the assignment of a putative functional category for 37% of sequences whereas ~ 16% sequences did not show any significant similarity with other proteins present in the database and therefore can be considered as cassava specific genes. A group of genes belonging to a large multigene family was identified. We characterize a set of genes detected only in infected libraries putatively involved in the defense response to pathogen infection. By comparing two libraries obtained from cultivars contrasting in their starch content a group of genes associated to starch biosynthesis and differentially expressed was identified. This is the first large cassava EST resource developed today and publicly available thus making a significant contribution to genomic knowledge of cassava.

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Affiliations

  1. Laboratoire Génome et Développement des Plantes, UMR5096, CNRS-Université de Perpignan – Institut de Recherche pour le Développement, 52 Av Paul Alduy, 66860, Perpignan Cedex, France

    Camilo Lopez, Véronique Jorge, Benoît Piégu, Michèle Laudié, Christel Berger, Richard Cooke, Michel Delseny & Valérie Verdier

  2. Centro Internacional de Agricultura Tropical, AA 6713, Cali, Colombia

    Chickelu Mba, Diego Cortes, Silvia Restrepo, Mauricio Soto & Joe Tohme

  3. INRA, Unité Amélioration Génétique et Physiologie Forestières, Av de la pomme de pin, BP 20619, Ardon, 45166, Olivet Cedex, France

    Véronique Jorge

  4. Plant Breeding Unit, Joint FAO/IAEA Agriculture and Biotechnology Laboratory, International Atomic Energy Agency Laboratories, A-2444, Seibersdorf, Austria

    Chickelu Mba

Authors
  1. Camilo Lopez
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  2. Véronique Jorge
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  3. Benoît Piégu
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  4. Chickelu Mba
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  5. Diego Cortes
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  6. Silvia Restrepo
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  7. Mauricio Soto
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  8. Michèle Laudié
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  9. Christel Berger
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  10. Richard Cooke
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  11. Michel Delseny
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  12. Joe Tohme
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  13. Valérie Verdier
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Corresponding author

Correspondence to Valérie Verdier.

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Lopez, C., Jorge, V., Piégu, B. et al. A unigene catalogue of 5700 expressed genes in cassava. Plant Mol Biol 56, 541–554 (2004). https://doi.org/10.1007/s11103-004-0123-4

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  • DOI: https://doi.org/10.1007/s11103-004-0123-4

Keywords

  • cassava bacterial blight
  • disease resistance
  • ESTs
  • gene expression
  • resistance gene
  • starch