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Expression of the long non-coding RNAs MEG3, HOTAIR, and MALAT-1 in non-functioning pituitary adenomas and their relationship to tumor behavior

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Abstract

Purpose

It is becoming evident that long non-coding RNAs (lncRNAs) participate in diverse biological processes via distinct mechanisms. Many lncRNAs have altered expression and likely to have functional roles in tumorigenesis. Although loss of maternally-expressed gene 3 (MEG3) expression has been detected in non-functioning pituitary adenomas (NFPAs), there are no published reports regarding the association between MEG3 expression and the invasive ability of NFPAs. Moreover, the roles of Hox transcript antisense intergenic RNA (HOTAIR) and metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) in NFPAs have not been examined. To investigate the role of MEG3, HOTAIR, and MALAT-1 in NFPA development and invasion.

Methods

MEG3, HOTAIR, MALAT-1 and proliferating cell nuclear antigen (PCNA) were detected in 52 NFPA samples and seven normal human anterior pituitaries using real-time quantitative reverse transcription polymerase chain reaction.

Results

MEG3 lncRNA levels gradually decreased whereas HOTAIR lncRNA levels gradually increased from normal anterior pituitaries to non-invasive NFPAs to invasive NFPAs. There was a significant association between MEG3 (P < 0.01) and HOTAIR (P < 0.05) expression and the biological behavior of the tumor. Furthermore, PCNA mRNA levels markedly increased in invasive NFPAs compared to non-invasive ones (P < 0.01). In addition, PCNA mRNA negatively correlated with MEG3 lncRNA levels (P < 0.05).

Conclusions

MEG3 and HOTAIR expression may correlate with NFPA development and invasion.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31000498).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Beijing Neurosurgical Institute, Capital Medical University, 6 Tiantan Xili, Dongcheng District, Beijing, 10050, People’s Republic of China

    Zhenye Li, Chuzhong Li, Chunhui Liu, Shengyuan Yu & Yazhuo Zhang

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  1. Zhenye Li
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Correspondence to Yazhuo Zhang.

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Li, Z., Li, C., Liu, C. et al. Expression of the long non-coding RNAs MEG3, HOTAIR, and MALAT-1 in non-functioning pituitary adenomas and their relationship to tumor behavior. Pituitary 18, 42–47 (2015). https://doi.org/10.1007/s11102-014-0554-0

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