Phytochemistry Reviews

, Volume 17, Issue 4, pp 937–946 | Cite as

Evaluation of the therapeutic effects of Artemisia absinthium L. on pseudopregnancy model in rats

  • Murşide Ayşe Demirel
  • Ipek Suntar
  • Songul Ceribaşı
  • Gökhan Zengin
  • Ali Osman Ceribaşı


Researchers have been investigating new treatment strategies for the management of pseudopregnancy, due to some long term causes of this disease including mastitis and mammary tumor. Owing to the side effects of hormone therapy, much attention has been focused on more tolerable treatment options including natural remedies. In the present study, we aimed to investigate the therapeutic effects of Artemisia absinthium L. (wormwood) on pseudopregnancy model in rats. A. absinthium, which is a perennial shrubby plant from Asteraceae family, was reported to be used as an emmenagogue in folk medicine and was previously shown to modulate dopaminergic system. In the present study, pseudopregnancy model was induced in female Sprague–Dawley rats by injection of pregnant mare’s serum gonadotropin and human chorionic gonadotropin. The petroleum ether, dichloromethane and methanol extracts of A. absinthium, prepared by successive extraction method, were orally administered to the rats at 100 mg/kg dose, once daily for 10 days. Bromocriptine (3 mg/kg/per os) was administered to the reference group animals. At the end of the experiment, all of the animals were sacrificed, the blood samples, ovaries and uterine tissues were taken for histopathological and biochemical analysis. According to the results of the present study, the petroleum ether extract displayed beneficial effects in pseudopregnancy model in rats when compared with the control group. Therefore, Gas Chromatography analysis was conducted on this extract to reveal its phytochemical profile.


Asteraceae Artemisia absinthium Dopaminergic Pseudopregnancy Rat 



Petroleum ether






Pregnant mare’s serum gonadotropin


Human chorionic gonadotropin


Gas chromatography-flame ionization detector


Gas chromatography/mass spectrometry


Carboxymethyl cellulose


Chemiluminescent microparticle immunoassay


Corpus luteum


Compliance with ethical standards

Conflict of interest

The authors declare that they have no potential conflicts of interest to disclose.


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory Animals Breeding and Experimental Researches Center, Department of Pharmacology, Faculty of PharmacyGazi UniversityEtiler, AnkaraTurkey
  2. 2.Department of Pharmacognosy, Faculty of PharmacyGazi UniversityEtiler, AnkaraTurkey
  3. 3.Department of Pathology, Faculty of Veterinary MedicineFırat UniversityElazigTurkey
  4. 4.Department of Biology, Science FacultySelcuk UniversityKonyaTurkey

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