Skip to main content

Advertisement

SpringerLink
Log in

Preliminary evidences of the direct and indirect antimicrobial activity of 12 plants used in traditional medicine in Africa

  • Published:
Phytochemistry Reviews Aims and scope Submit manuscript

Abstract

In a world of increasing resistance to current antibiotics, search of novel therapeutic options is urgently needed. The aim of this work was to screen plant crude extracts for direct or indirect (inhibition of resistance) antimicrobial activity. Four crude extracts from 12 plants traditionally used in Africa for the treatment of infections were obtained by successive extraction with hexane, dichloromethane, ethyl acetate, and methanol. All extracts were tested against Staphylococcus aureus MRSA ATCC33591 [resistant to β-lactams by production of β-lactamases and of a modified PBP target (PBP2a)]. Direct antimicrobial activity was tested by determination of Minimal Inhibitory Concentrations (MIC), and indirect activity, by determining interactions between antibiotics and extracts using checkerboard titration and calculation of Fractional Inhibitory Concentration Index (FICI; synergy: FICI ≤ 0.5; additivity: FICI ≤ 1). Combined antibiotics were ampicillin (sensitive to resistance mediated by β-lactamases and PBP2a) and oxacillin (sensitive to resistance mediated by PBP2a only). The dichloromethane extract of Vitellaria paradoxa leaves, the methanol extracts of Vitellaria paradoxa, Cola gigantea leaves and twigs, and of Tapinanthus bangwensis aerial parts showed direct antimicrobial activity (MIC 250–500 mg/L). The methanol extracts of Vitellaria paradoxa and Cola gigantea leaves and twigs showed additive or synergistic effects with oxacillin and ampicillin on MRSA ATCC33591 (FICI 0.28–1), suggesting a possible inhibition of PBP2a. The methanol extract of Tapinanthus bangwensis aerial parts and Anchomanes difformis roots improved the activity of ampicillin only (FICI 0.38–1), suggesting β-lactamase inhibition. Polyphenols and particularly tannins were shown to be responsible for these last effects, at least partially for Vitellaria paradoxa. These data need further research aiming at identifying the active compounds in these extracts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abah SE, Egwari L, Mosaku TO (2011) In vitro antimicrobial screening on Anchomanes difformis (Blume) Engl. leaves and rhizomes against selected pathogens of public health importance. Adv Biol Res 5:221–225

    Google Scholar 

  • Abreu AC, McBain AJ, Simões M (2012) Plants as sources of new antimicrobials and resistance-modifying agents. Nat Prod Rep 29:1007–1021. doi:10.1039/C2NP20035J

    Article  CAS  PubMed  Google Scholar 

  • Adeleke O, Adetunji T (2010) Antimicrobial activity of Anchomanes difformis (Blume) Engl. [family ARACEAE]. J Life Phys Sci 2:87–90

    Google Scholar 

  • Adjanohoun EJ, Adjakidje V, Ahyi MR, Aké assi L, Akoegninou A, d’Almeida J, Apovo F, Boukef K, Chadare M, Cusset G, Dramane K, Eyme J, Gassita JN, Gbaguidi N, Goudote E, Guinko P, Houngnon P, Lo I, Keita A, Kiiniffo HV, Kone-Bamba D, Musampa Nseyya A, Saadou M, Sodogandji T, de Souza S, Tchabi A, Zinsou T (1989) Contribution aux études ethnobotaniques et floristiques en République Populaire du Bénin. In: Médecine Traditionnelle et Pharmacopée. Cotonou

  • Agbo MO, Lai D, Okoye FBC, Osadebe PO, Proksch P (2013) Antioxidative polyphenols from Nigerian mistletoe Loranthus micranthus (Linn.) parasitizing on Hevea brasiliensis. Fitoterapia 86:78–83. doi:10.1016/j.fitote.2013.02.006

    Article  CAS  PubMed  Google Scholar 

  • Agyare C, Koffuor GA, Boamah VE, Adu F, Mensah KB, Adu-Amoah L (2012) Antimicrobial and anti-inflammatory activities of Pterygota macrocarpa and Cola gigantea (Sterculiaceae). Evid Based Complement Altern Med 2012:e902394. doi:10.1155/2012/902394

    Article  Google Scholar 

  • Ahua KM, Ioset J-R, Diallo D, Mauël J, Hostettmann K (2007) Antileishmanial activities associated with plants used in the Malian traditional medicine. J Ethnopharmacol 110:99–104

    Article  PubMed  Google Scholar 

  • Aliyu AB, Musa AM, Oshanimi JA, Ibrahim HA, Oyewale AO (2008) Phytochemical analyses and mineral elements composition of some medicinal plants of Northern Nigeria. Niger J Pharm Sci 7:119–125

    Google Scholar 

  • Aniszewski T (2007) Alkaloids: secrets of life: aklaloid chemistry, biological significance, applications and ecological role. Elsevier, Amsterdam

    Google Scholar 

  • Asase A, Kokubun T, Grayer RJ, Kite G, Simmonds MSJ, Oteng-Yeboah AA, Odamtten GT (2008) Chemical constituents and antimicrobial activity of medicinal plants from Ghana: Cassia sieberiana, Haematostaphis barteri, Mitragyna inermis and Pseudocedrela kotschyi. Phytother Res 22:1013–1016. doi:10.1002/ptr.2392

    Article  CAS  PubMed  Google Scholar 

  • Ayankunle AA, Kolawole OT, Adesokan AA, Akiibinu MO (2012) Antibacterial activity and sub-chronic toxicity studies of vitellaria paradoxa stem bark extract. J Pharmacol Toxicol 7:298–304. doi:10.3923/jpt.2012.298.304

    Article  Google Scholar 

  • Badmus JA, Odunola OA, Obuotor EM, Oyedapo OO (2009) Phytochemicals and in vitro antioxidant potentials of defatted methanolic extract of Holarrhena floribunda leaves. Afr J Biotechnol 9:340–346

    Google Scholar 

  • Balogun SO, Oladosu IA, Liu Z (2014) Chemical compositions and antioxidant potential of essential oils from leaves and flowers of Allophylus africanus. J Essent Oil Bear Plants 17:769–775. doi:10.1080/0972060X.2014.895176

    Article  CAS  Google Scholar 

  • Barber M, Waterworth PM (1964) Penicillinase-resistant Penicillins. Br Med J 2:344–349

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Bero J, Ganfon H, Jonville M-C, Frédérich M, Gbaguidi F, DeMol P, Moudachirou M, Quetin-Leclercq J (2009) In vitro antiplasmodial activity of plants used in Benin in traditional medicine to treat malaria. J Ethnopharmacol 122:439–444. doi:10.1016/j.jep.2009.02.004

    Article  PubMed  Google Scholar 

  • Bogne KP, Penlap BV, Lontsi D, Etoa F-X (2007) Antibacterial activities of the extracts and conessine from Holarrhena floribunda G. Don. (Apocynaceae). Afr J Tradit Complement Altern Med 4:352–356

    PubMed  Google Scholar 

  • Bonapace CR, Bosso JA, Friedrich LV, White RL (2002) Comparison of methods of interpretation of checkerboard synergy testing. Diagn Microbiol Infect Dis 44:363–366. doi:10.1016/S0732-8893(02)00473-X

    Article  PubMed  Google Scholar 

  • Bruneton J (2009) Pharmacognosie, phytochimie, plantes médicinales (4e ed.), Tec & Doc ed Lavoisier, Paris

  • Buyck JM, Tulkens PM, Van Bambeke F (2015) Activities of antibiotic combinations against resistant strains of Pseudomonas aeruginosa in a model of infected THP-1 monocytes. Antimicrob Agents Chemother 59:258–268. doi:10.1128/AAC.04011-14

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Cha J-D, Moon S-E, Kim J-Y, Jung E-K, Lee Y-S (2009) Antibacterial activity of sophoraflavanone G isolated from the roots of Sophora flavescens against methicillin-resistant Staphylococcus aureus. Phytother Res 23:1326–1331. doi:10.1002/ptr.2540

    Article  CAS  PubMed  Google Scholar 

  • Chanda S, Rakholiya K (2011) Indian combination therapy: synergism between natural plant extracts and antibiotics against infectious diseases. In: Mendez-Vilas A (ed) Science against microbial pathogens: communicating current research and technological advances. Formatex Research Center, Spain, pp 520–529

    Google Scholar 

  • Chen H-Y, Ye X-L, Cui X-L, He K, Jin Y-N, Chen Z, Li X-G (2012) Cytotoxicity and antihyperglycemic effect of minor constituents from rhizoma coptis in HepG2 cells. Fitoterapia 83:67–73. doi:10.1016/j.fitote.2011.09.014

    Article  CAS  PubMed  Google Scholar 

  • Chinworrungsee M, Wiyakrutta S, Sriubolmas N, Chuailua P, Suksamrarn A (2008) Cytotoxic activities of trichothecenes isolated from an endophytic fungus belonging to order Hypocreales. Arch Pharm Res 31:611–616. doi:10.1007/s12272-001-1201-x

    Article  CAS  PubMed  Google Scholar 

  • Chung K-T, Stevens SE Jr, Lin W-F, Wei CI (1993) Growth inhibition of selected food-borne bacteria by tannic acid, propyl gallate and related compounds. Lett Appl Microbiol 17:29–32. doi:10.1111/j.1472-765X.1993.tb01428.x

    Article  CAS  Google Scholar 

  • Coates A, Hu Y, Bax R, Page C (2002) The future challenges facing the development of new antimicrobial drugs. Nat Rev Drug Discov 1:895–910. doi:10.1038/nrd940

    Article  CAS  PubMed  Google Scholar 

  • Comini LR, Núñez Montoya SC, Páez PL, Argüello GA, Albesa I, Cabrera JL (2011) Antibacterial activity of anthraquinone derivatives from Heterophyllaea pustulata (Rubiaceae). J Photochem Photobiol, B 102:108–114. doi:10.1016/j.jphotobiol.2010.09.009

    Article  CAS  Google Scholar 

  • Cowan MM (1999) Plant Products as Antimicrobial Agents. Clin Microbiol Rev 12:564–582

    PubMed Central  CAS  PubMed  Google Scholar 

  • Cushnie TPT, Lamb AJ (2005) Antimicrobial activity of flavonoids. Int J Antimicrob Agents 26:343–356. doi:10.1016/j.ijantimicag.2005.09.002

    Article  CAS  PubMed  Google Scholar 

  • Dilika F, Bremner PD, Meyer JJM (2000) Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: a plant used during circumcision rites. Fitoterapia 71:450–452. doi:10.1016/S0367-326X(00)00150-7

    Article  CAS  PubMed  Google Scholar 

  • Doyinsola Idowu, Adedayo Adebiyi, Olutayo Olajide, Michael Afolayan, Abayomi Orishadipe, Moses Omojola, Thomas Sunday (2012) Phytochemical, antioxidant and cytotoxicity properties of Anchomanes difformis (Bl.) Engl. tuber extract. Int J Appl Chem 8:173–181

    Google Scholar 

  • Drawz SM, Bonomo RA (2010) Three decades of beta-lactamase inhibitors. Clin Microbiol Rev 23:160–201. doi:10.1128/CMR.00037-09

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Dua VK, Verma G, Singh B, Rajan A, Bagai U, Agarwal DD, Gupta NC, Kumar S, Rastogi A (2013) Anti-malarial property of steroidal alkaloid conessine isolated from the bark of Holarrhena antidysenterica. Malar. J. 12:194. doi:10.1186/1475-2875-12-194

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Efuntoye MO, Ayodele AE, Thomas BT, Ajayi TO (2010) Does host plant affect the antibacterial activity of Tapinanthus bangwensis (Engl. and K. Krause) Danser (Loranthaceae). J Med Plants Res 4:1281–1284

    Google Scholar 

  • Ekhaise FO, Ofoezie VG, Enobakhare DA (2010) Antibacterial properties and preliminary phytochemical analysis of methanolic extract of mistletoe (Tapinanthus bangwensis). Bayero J Pure Appl Sci 3:65–68. doi:10.4314/bajopas.v3i2.63223

    Google Scholar 

  • Eliopoulos G, Moellering RC (1996) Antimicrobial combinations. Antibiotics in laboratory medicine. Lorian V, Baltimore, pp 330–396

    Google Scholar 

  • El-Mahmood AM, Doughari JH, Ladan N (2008) Antimicrobial screening of stem bark extracts of Vitellaria paradoxa against some enteric pathogenic microorganisms. Afr J Pharm Pharmacol 2:089–094

    Google Scholar 

  • EUCAST (2000) Terminology relating to methods for the determination of susceptibility of bacteria to antimicrobial agents. Clin Microbiol Infect 6:503–508. doi:10.1046/j.1469-0691.2000.00149.x

    Article  Google Scholar 

  • Fotie J, Bohle DS, Leimanis ML, Georges E, Rukunga G, Nkengfack AE (2006) Lupeol long-chain fatty acid esters with antimalarial activity from Holarrhena floribunda. J Nat Prod 69:62–67. doi:10.1021/np050315y

    Article  CAS  PubMed  Google Scholar 

  • Fu J, Cheng K, Zhang Z, Fang R, Zhu H (2010) Synthesis, structure and structure–activity relationship analysis of caffeic acid amides as potential antimicrobials. Eur J Med Chem 45:2638–2643. doi:10.1016/j.ejmech.2010.01.066

    Article  CAS  PubMed  Google Scholar 

  • Garba S, Salihu L (2011) Antibacterial activities of 2-O-butyl-1-O-(2′-ethylhexyl) benzene-1,8-dicarboxylate and 1-phenyl-1,4-pentanedione isolated from Vitellaria paradoxa root bark. Asian J Sci Res 4:149–157. doi:10.3923/ajsr.2011.149.157

    Article  CAS  Google Scholar 

  • Gershenzon J (1984) Changes in the levels of plant secondary metabolites under water and nutrient stress. In: Timmermann BN, Steelink C, Loewus FA (eds) Phytochemical adaptations to stress, recent advances in phytochemistry. Springer, New York, pp 273–320

    Chapter  Google Scholar 

  • Gibbons S (2004) Anti-staphylococcal plant natural products. Nat Prod Rep 21:263–277. doi:10.1039/B212695H

    Article  CAS  PubMed  Google Scholar 

  • Gibbons S (2005) Plants as a source of bacterial resistance modulators and anti-infective agents. Phytochem Rev 4:63–78. doi:10.1007/s11101-005-2494-9

    Article  CAS  Google Scholar 

  • Hadler JL, Petit S, Mandour M, Cartter M (2012) Trends in Invasive Infection with Methicillin-Resistant Staphylococcus aureus, Connecticut, USA, 2001–2010. Emerg. Infect. Dis. J. 18:917–924

    Article  Google Scholar 

  • Hall JB, Aebischer DP, Tomlinson HF, Osei-Amaning E, Hindle JR (1996) Vitellaria paradoxa: a monograph. School of Agricultural and Forest Sciences, University of Wales

  • Hemaiswarya S, Kruthiventi AK, Doble M (2008) Synergism between natural products and antibiotics against infectious diseases. Phytomedicine 15:639–652. doi:10.1016/j.phymed.2008.06.008

    Article  CAS  PubMed  Google Scholar 

  • Houghton PJ, Raman A (1998) Laboratory handbook for the fractionation of natural extracts. Chapman and Hall, London

    Book  Google Scholar 

  • Janot MM, Cave A, Goutarel R (1960) Steroid alkaloids. Holaphyllamine and holamine, alkaloids from Holarrhena floribunda (G. Don) Dur. and Schinz. Comptes Rendus Hebd. Séances Académie Sci. 251:559–561

    CAS  Google Scholar 

  • Jiofack T, Fokunang C, Guedje N, Kemeuze V, Fongnzossie E, Nkongmeneck BA, Mapongmetsem PM, Tsabang N (2010) Ethnobotanical uses of medicinal plants of two ethnoecological regions of Cameroon. Int J Med Med Sci 2:60–79

    Google Scholar 

  • Johnson AP (2011) Methicillin-resistant Staphylococcus aureus: the European landscape. J Antimicrob Chemother 66:43–48. doi:10.1093/jac/dkr076

    Google Scholar 

  • Kalant H (1965) The pharmacology of semisynthetic antibiotics. Can Med Assoc J 93:839–843

    PubMed Central  CAS  PubMed  Google Scholar 

  • Kamagaju L (2014) Problem of skin depigmentation in Rwanda. Modulators of tyrosinase extracted from plants used in traditional medicine. Thesis in Biomedical and Pharmaceutical Sciences, ULB, Brussels

  • Karou D, Savadogo A, Canini A, Yameogo S, Montesano C, Simpore J, Colizzi V, Traore AS (2005) Antibacterial activity of alkaloids from Sida acuta. Afr J Biotechnol. doi:10.4314/ajb.v4i12.71463

    Google Scholar 

  • Kim HS, Han SS, Oh KW, Jeong TS, Nam KY (1987) Effects of Ginseng saponin on the antimicrobial activities of some antibiotics. Korean J Mycol 15:87–91

    CAS  Google Scholar 

  • Kuete V (2010) Potential of Cameroonian plants and derived products against microbial infections: a review. Planta Med 76:1479–1491. doi:10.1055/s-0030-1250027

    Article  CAS  PubMed  Google Scholar 

  • Lagnika L, Weniger B, Attioua B, Jensen O, Anthaume C, Sanni A, Kaiser M, Lobstein A, Vonthron-Senecheau C (2012) Trypanocidal activity of diarylheptanoids from Schrankia leptocarpa DC. South Afr J Bot 83:92–97. doi:10.1016/j.sajb.2012.06.011

    Article  CAS  Google Scholar 

  • Latifou Lagnika UP (2012) Chemical analysis, antimicrobial and antioxidant activities of eight extracts from Schrankia leptocarpa L. Afr J Biotechnol 11:13739–13745. doi:10.5897/AJB12.633

    Google Scholar 

  • Leboeuf M, Cavé A, Goutarel R (1969) Steroid alkaloids. LXXXII. (16). Chemical composition of the leaves of Holarrhena floribunda Dur. et Schinz. Isolation of progesterone and 4 new alkaloids: methylholaphylline, holaphyllinol, holaphyllidine and dihydroholaphyllamine. Ann Pharm Fr 27:217–228

    CAS  PubMed  Google Scholar 

  • Lee Y-S, Kang O-H, Choi J-G, Oh Y-C, Keum J-H, Kim S-B, Jeong G-S, Kim Y-C, Shin D-W, Kwon D-Y (2010) Synergistic effect of emodin in combination with ampicillin or oxacillin against methicillin-resistant Staphylococcus aureus. Pharm Biol 48:1285–1290. doi:10.3109/13880201003770150

    Article  CAS  PubMed  Google Scholar 

  • Loukaci A, Kayser O, Bindseil K, Siems K, Frevert J, Abreu PM (2000) New trichothecenes isolated from Holarrhena floribunda. J Nat Prod 63:52–56

    Article  CAS  PubMed  Google Scholar 

  • Lowy FD (1998) Staphylococcus aureus infections. N Engl J Med 339:520–532. doi:10.1056/NEJM199808203390806

    Article  CAS  PubMed  Google Scholar 

  • Manríquez-Torres J, Zúñiga-Estrada A, González-Ledesma M, Torres-Valencia JM (2007) The antibacterial metabolites and proacacipetalin from Acacia cochliacantha. J Mex Chem Soc 51:228–231

    Google Scholar 

  • Mathabe MC, Hussein AA, Nikolova RV, Basson AE, Meyer JJM, Lall N (2008) Antibacterial activities and cytotoxicity of terpenoids isolated from Spirostachys africana. J Ethnopharmacol 116:194–197. doi:10.1016/j.jep.2007.11.017

    Article  CAS  PubMed  Google Scholar 

  • Mbouangouere RN, Tane P, Ngamga D, Khan SN, Choudhary MI, Ngadjui BT (2007) A new steroid and α-glucosidase inhibitors from Anthocleista schweinfurthii. Res J Med Plant 1:106–111

    Article  CAS  Google Scholar 

  • Mezui C, Longo F, Nkenfou C, Sando Z, Ndeme E, Vernyuy Tan P (2015) Evalutation of acute and subacute tovicity of stem bark aqueous extract of Anthocleista schweinfurthii (Loganiaceae). World J Pharm Pharm Sci 4:197–208

    Google Scholar 

  • Miller RR, Price JR, Batty EM, Didelot X, Wyllie D, Golubchik T, Crook DW, Paul J, Peto TEA, Wilson DJ, Cule M, Ip CLC, Day NPJ, Moore CE, Bowden R, Llewelyn MJ (2014) Healthcare-associated outbreak of meticillin-resistant Staphylococcus aureus bacteraemia: role of a cryptic variant of an epidemic clone. J Hosp Infect 86:83–89. doi:10.1016/j.jhin.2013.11.007

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Moriarity DM, Huang J, Yancey CA, Zhang P, Setzer WN, Lawton RO, Bates RB, Caldera S (1998) Lupeol is the cytotoxic principle in the leaf extract of Dendropanax cf. querceti. Planta Med 64:370–372

    Article  CAS  PubMed  Google Scholar 

  • Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    Article  CAS  PubMed  Google Scholar 

  • Ndukwe IG, Amupitan JO, Isah Y, Adegoke KS (2007) Phytochemical and antimicrobial screening of the crude extracts from the root, stem bark and leaves of Vitellaria paradoxa (Gaertn. F). Afr J Biotechnol 6:1905–1909

    CAS  Google Scholar 

  • Ngbolua KN, Mubindoukila Rosie Esther N, Mpiana Pius T, Ashande Masengo C, Robijaona Baholy, Ruphin Fatiany Pierre, Takoy L, Ekutsu Grégoire E, Gbolo Zoawe B (2014) In vitro assessment of antibacterial and antioxidant activities of a congolese medicinal plant species Anthocleista schweinfurthii Gilg (Gentianaceae). J Mod Drug Discov Drug Deliv Res 3:1–6. doi:10.15297/JMDDR.V1I3.03

    Google Scholar 

  • Njayou FN, Moundipa PF, Tchana AN, Ngadjui BT, Tchouanguep FM (2008) Inhibition of microsomal lipid peroxidation and protein oxidation by extracts from plants used in Bamun folk medicine (Cameroon) against hepatitis. Afr J Tradit Complement Altern Med 5:278–289

    PubMed Central  CAS  PubMed  Google Scholar 

  • Nondo RSO, Mbwambo ZH, Kidukuli AW, Innocent EM, Mihale MJ, Erasto P, Moshi MJ (2011) Larvicidal, antimicrobial and brine shrimp activities of extracts from Cissampelos mucronata and Tephrosia villosa from coast region, Tanzania. BMC Complement Altern Med 11:33. doi:10.1186/1472-6882-11-33

    Article  PubMed  Google Scholar 

  • Nwodo NJ, Brun R, Osadebe PO (2007) In vitro and in vivo evaluation of the antitrypanosomal activity of fractions of Holarrhena africana. J Ethnopharmacol 113:556–559. doi:10.1016/j.jep.2007.07.018

    Article  CAS  PubMed  Google Scholar 

  • Odiba JO, Musa AM, Hassan HS, Yahaya SM, Okolo EI (2014) Antimicrobial activity of isolated Stigmast-5-en-3β-ol (β-sitosterol) from honeybee propolis from North-Western, Nigeria. Int J Pharma Sci Res 5:908–918

    Google Scholar 

  • Ogechukwu OE, Ogoamaka OP, Sylvester NC, Hassan A, Debbab A, Okechukwu EC, Kawamura A, Peter P (2011) Steroids and triterpenoids from Eastern Nigeria mistletoe, Loranthus micranthus Linn. (Loranthaceae) parasitic on Kola acuminata with immunomodulatory potentials. Phytochem Lett 4:357–362. doi:10.1016/j.phytol.2011.07.011

    CAS  Google Scholar 

  • Ogechukwu OE, Ogoamaka OP, Kawamura A, Hassan A, Debbab A et al (2012) Three-(−) catechin-O-rhamnosides from the Eastern Nigeria Mistletoe with potent immunostimulatory and antioxidant activities. Biomolecul 1:2

    Article  Google Scholar 

  • Ogunwande IA, Bello MO, Olawore ON, Muili KA (2001) Phytochemical and antimicrobial studies on Butyrospermum paradoxum. Fitoterapia 72:54–56. doi:10.1016/S0367-326X(00)00238-0

    Article  CAS  PubMed  Google Scholar 

  • Oladosu IA, Balogun SO, Ademowo GO (2013) Phytochemical screening, antimalarial and histopathological studies of Allophylus africanus and Tragia benthamii. Chin. J. Nat. Med. 11:371–376. doi:10.1016/S1875-5364(13)60054-0

    Article  CAS  PubMed  Google Scholar 

  • Oladosu IA, Balogun SO, Liu Z-Q (2015) Chemical constituents of Allophylus africanus. Chin. J. Nat. Med. 13:133–141. doi:10.1016/S1875-5364(15)60017-6

    CAS  PubMed  Google Scholar 

  • Olutayo Olajide, Michael Afolayan, John Adewusi Adepoju, Olusola Adeyanju (2012) Antimicrobial activity and Elemental analysis of Cassia siberiana leaves Using Atomic Absorption Spectrometer. J. Nat. Prod. Plant Resour. 2:9–18

    Google Scholar 

  • Omeje EO, Osadebe PO, Nworu CS, Nwodo JN, Obonga WO, Kawamura A, Esimone CO, Proksch P (2011) A novel sesquiterpene acid and an alkaloid from leaves of the Eastern Nigeria mistletoe, Loranthus micranthus with potent immunostimulatory activity on C57BL6 mice splenocytes and CD69 molecule. Pharm Biol 49:1271–1276. doi:10.3109/13880209.2011.621129

    Article  CAS  PubMed  Google Scholar 

  • Onyema CT, Ajiwe VIE (2014a) Phytochemical and anti-microbial analysis of the leaves of Cola gigantea (Sterculiaceae). Int J Appl Sci Technol 4:119–126

    Google Scholar 

  • Onyema CT, Ajiwe VIE (2014b) Phytochemical and antimicrobial analysis of the stems of Cola gigantea (Sterculiacea). Int J Eng Sci 3:01–11

    Google Scholar 

  • Otsuka N, Liu M-H, Shiota S, Ogawa W, Kuroda T, Hatano T, Tsuchiya T (2008) Anti-methicillin resistant Staphylococcus aureus (MRSA) compounds isolated from Laurus nobilis. Biol Pharm Bull 31:1794–1797

    Article  CAS  PubMed  Google Scholar 

  • Oyetayo VO (2007) Comparative studies of the phytochemical and antimicrobial properties of the leaf, stem and tuber of Anchomanes difformis. J Pharmacol Toxicol 2:407–410. doi:10.3923/jpt.2007.407.410

    Article  Google Scholar 

  • Paris R, Etchepare S (1967) On the polyphenols of Cassia sieberiana DC. Isolation of l-epicatechol and leucopelargonidol. Ann Pharm Fr 25:343–346

    PubMed  Google Scholar 

  • Paris RR, Foucaud A (1959) Flavonoids in the leaves of Holarrhena floribunda G. Don (Durand and Schinz): isolation of a flavonoside identified as isoquercitroside. Comptes Rendus Hebd Séances Académie Sci 248:2634–2635

    CAS  Google Scholar 

  • Patrick-Iwuanyanwu KC, Onyeike EN, Adhikari A (2014) Isolation, identification and characterization of gallic acid derivatives from leaves of Tapinanthus bangwensis. J Nat Prod 7:14–19

    Google Scholar 

  • Raoelison EG, Rafamantanana MH, Razafindrazaka R, Randriantsoa A, Urverg-Ratsimamanga S, Morel N, Quetin-Leclercq J (2013) Vasorelaxant alkaloids from Spirospermum penduliflorum (Menispermaceae), a plant used to treat hypertension in malagasy traditional medicine. Nat Prod Commun Int J Commun Rev 8:575–578

    CAS  Google Scholar 

  • Ratsimamanga-Urverg S, Rasoanaivo P, Ramiaramanana L, Milijaona R, Rafatro H, Verdier F, Rakoto-Ratsimamanga A, Le Bras J (1992) In vitro antimalarial activity and chloroquine potentiating action of two bisbenzylisoquinoline enantiomer alkaloids isolated from Strychnopsis thouarsii and Spirospermum penduliflorum. Planta Med 58:540–543. doi:10.1055/s-2006-961545

    Article  CAS  PubMed  Google Scholar 

  • Rodvold KA, McConeghy KW (2014) Methicillin-resistant Staphylococcus aureus therapy: past, present, and future. Clin Infect Dis 58:S20–S27. doi:10.1093/cid/cit614

    Article  CAS  PubMed  Google Scholar 

  • Russell KM, Molan PC, Wilkins AL, Holland PT (1990) Identification of some antibacterial constituents of New Zealand manuka honey. J Agric Food Chem 38:10–13. doi:10.1021/jf00091a002

    Article  CAS  Google Scholar 

  • Sandora TJ, Goldmann DA (2012) Preventing lethal hospital outbreaks of antibiotic-resistant bacteria. N Engl J Med 367:2168–2170. doi:10.1056/NEJMp1212370

    Article  CAS  PubMed  Google Scholar 

  • Santiago C, Pang EL, Lim K-H, Loh H-S, Ting KN (2014) Reversal of ampicillin resistance in MRSA via inhibition of penicillin-binding protein 2a by Acalypha wilkesiana. BioMed Res Int. doi:10.1155/2014/965348

    Google Scholar 

  • Sarker SD, Nahar L, Kumarasamy Y (2007) Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods Nat Prod Res 42:321–324. doi:10.1016/j.ymeth.2007.01.006

    CAS  Google Scholar 

  • Schmelzer GH, Gurib-Fakim A (2008) Ressources végétales de l’Afrique tropicale 11 (1): Plantes médicinales. Schmelzer G.H & Gurib-Fakim A, Pay-Bas

    Google Scholar 

  • Shibata H, Kondo K, Katsuyama R, Kawazoe K, Sato Y, Murakami K, Takaishi Y, Arakaki N, Higuti T (2005) Alkyl gallates, intensifiers of β-lactam susceptibility in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 49:549–555. doi:10.1128/AAC.49.2.549-555.2005

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Shimizu M, Shiota S, Mizushima T, Ito H, Hatano T, Yoshida T, Tsuchiya T (2001) Marked potentiation of activity of β-lactams against methicillin-resistant Staphylococcus aureus by corilagin. Antimicrob Agents Chemother 45:3198–3201. doi:10.1128/AAC.45.11.3198-3201.2001

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Shiota S, Shimizu M, Mizushima T, Ito H, Hatano T, Yoshida T, Tsuchiya T (1999) Marked reduction in the minimum inhibitory concentration (MIC) of beta-lactams in methicillin-resistant Staphylococcus aureus produced by epicatechin gallate, an ingredient of green tea (Camellia sinensis). Biol Pharm Bull 22:1388–1390

    Article  CAS  PubMed  Google Scholar 

  • Shiota S, Shimizu M, Mizusima T, Ito H, Hatano T, Yoshida T, Tsuchiya T (2000) Restoration of effectiveness of β-lactams on methicillin-resistant Staphylococcus aureus by tellimagrandin I from rose red. FEMS Microbiol Lett 185:135–138. doi:10.1111/j.1574-6968.2000.tb09051.x

    CAS  PubMed  Google Scholar 

  • Shiota S, Shimizu M, Sugiyama J, Morita Y, Mizushima T, Tsuchiya T (2004) Mechanisms of action of corilagin and tellimagrandin I that remarkably potentiate the activity of beta-lactams against methicillin-resistant Staphylococcus aureus. Microbiol Immunol 48:67–73

    Article  CAS  PubMed  Google Scholar 

  • Sibanda T, Okoh AI (2007) The challenges of overcoming antibiotic resistance: plant extracts as potential sources of antimicrobial and resistance modifying agents. Afr J Biotechnol. doi:10.4314/ajb.v6i25.58241

    Google Scholar 

  • Sofidiya MO, Jimoh Florence O, Aliero Adamu A, Afolayan Anthony J, Odukoya Olukemi A, Familoni Oluwole B (2012) Evaluation of antioxidant and antibacterial properties of six Sapindaceae members. J Med Plants Res. doi:10.5897/JMPR11.1364

    Google Scholar 

  • Sonibare M, Soladoye M, Esan O, Sonibare O (2009) Phytochemical and antimicrobial studies of four species of Cola Schott & Endl. (Sterculiaceae). Afr J Tradit Complement Altern Med 6:518–525

    PubMed Central  CAS  PubMed  Google Scholar 

  • Stapleton PD, Shah S, Anderson JC, Hara Y, Hamilton-Miller JMT, Taylor PW (2004) Modulation of β-lactam resistance in Staphylococcus aureus by catechins and gallates. Int J Antimicrob Agents 23:462–467. doi:10.1016/j.ijantimicag.2003.09.027

    Article  CAS  PubMed  Google Scholar 

  • Stapleton PD, Shah S, Ehlert K, Hara Y, Taylor PW (2007) The β-lactam-resistance modifier (–)-epicatechin gallate alters the architecture of the cell wall of Staphylococcus aureus. Microbiol Read Engl 153:2093–2103. doi:10.1099/mic.0.2007/007807-0

    Article  CAS  Google Scholar 

  • Stevigny C, Block S, De Pauw-Gillet MC, de Hoffmann E, Llabrès G, Adjakidjé V, Quetin-Leclercq J (2002) Cytotoxic aporphine alkaloids from Cassytha filiformis. Planta Med 68:1042–1044. doi:10.1055/s-2002-35651

    Article  CAS  PubMed  Google Scholar 

  • Stévigny C, Block S, De Pauw-Gillet MC, de Hoffmann E, Llabrès G, Adjakidjé V, Quetin-Leclercq J (2002) Cytotoxic aporphine alkaloids from Cassytha filiformis. Planta Med 68:1042–1044. doi:10.1055/s-2002-35651

    Article  PubMed  Google Scholar 

  • Surup F, Medjedović A, Szczygielski M, Schroers H-J, Stadler M (2014) Production of trichothecenes by the apple sooty blotch fungus Microcyclospora tardicrescens. J Agric Food Chem 62:3525–3530. doi:10.1021/jf500153d

    Article  CAS  PubMed  Google Scholar 

  • Tagne RS, Telefo BP, Nyemb JN, Yemele DM, Njina SN, Goka SMC, Lienou LL, Nwabo Kamdje AH, Moundipa PF, Farooq AD (2014) Anticancer and antioxidant activities of methanol extracts and fractions of some Cameroonian medicinal plants. Asian Pac J Trop Med 7(S1):S442–S447. doi:10.1016/S1995-7645(14)60272-8

    Article  Google Scholar 

  • Tanko Y, Yaro AH, Isa AI, Yerima M, Saleh MIA, Mohammed A (2007) Toxicological and hypoglycemic studies on the leaves of Cissampelos mucronata (Menispermaceae) on blood glucose levels of streptozocin-induced diabetic wistar rats. J Med Plants Res 1:113–116

    Google Scholar 

  • Tapondjou LA, Nyaa LBT, Tane P, Ricciutelli M, Quassinti L, Bramucci M, Lupidi G, Ponou BK, Barboni L (2011) Cytotoxic and antioxidant triterpene saponins from Butyrospermum parkii (Sapotaceae). Carbohydr Res 346:2699–2704. doi:10.1016/j.carres.2011.09.014

    Article  CAS  PubMed  Google Scholar 

  • Taylor PW (2013) Alternative natural sources for a new generation of antibacterial agents. Int J Antimicrob Agents 42:195–201. doi:10.1016/j.ijantimicag.2013.05.004

    Article  CAS  PubMed  Google Scholar 

  • Tchiapke L, Balansard G, Bernard P, Placidi M (1980) Chemical and toxicological study of Anchomanes difformis (Engl). Herba Hung 19:55–63

    Google Scholar 

  • Tenover FC (2006) Mechanisms of antimicrobial resistance in bacteria. Am J Med 119:S3–S10. doi:10.1016/j.amjmed.2006.03.011

    Article  CAS  PubMed  Google Scholar 

  • Tshibangu JN, Wright AD, König GM (2003) HPLC isolation of the anti-plasmodially active bisbenzylisoquinone alkaloids present in roots of Cissampelos mucronata. Phytochem Anal 14:13–22. doi:10.1002/pca.673

    Article  CAS  PubMed  Google Scholar 

  • Udegbunam SO, Udegbunam RI, Okpo OS, Muogbo CJ (2012) Evaluation of the wound healing potential of Protea madiensis olive leaf extract in rats. Afr J Biotechnol 11:15719–15727. doi:10.5897/AJB12.517

    Article  Google Scholar 

  • Wagner H, Bladt S (1996) Plant drug analysis: a thin layer chromatography atlas. Springer, Berlin

    Book  Google Scholar 

  • Yemoa A, Gbenou J, Affolabi D, Moudachirou M, Bigot A, Anagonou S, Portaels F, Martin A, Quetin-Leclercq J (2014) Beninese medicinal plants as a source of antimycobacterial agents: bioguided fractionation and in vitro activity of alkaloids isolated from Holarrhena floribunda used in traditional treatment of buruli ulcer. BioMed Res Int. doi:10.1155/2015/835767

    Google Scholar 

  • Yotis W, Stanke R (1966) Bacteriostatic action of progesterone on Staphylococci and other microorganisms. J Bacteriol 92:1285–1289

    PubMed Central  CAS  PubMed  Google Scholar 

  • Yu H-H, Kim K-J, Cha J-D, Kim H-K, Lee Y-E, Choi N-Y, You Y-O (2005) Antimicrobial activity of berberine alone and in combination with ampicillin or oxacillin against methicillin-resistant Staphylococcus aureus. J Med Food 8:454–461. doi:10.1089/jmf.2005.8.454

    Article  CAS  PubMed  Google Scholar 

  • Zerbo P, Millogo-Rasolodimby J, Nacoulma-Ouedraogo OG, Van Damme P (2011) Plantes médicinales et pratiques médicales au Burkina Faso : cas des Sanan. Bois For Trop 1:41–53

    Google Scholar 

Download references

Acknowledgments

The authors are grateful to Mr. Agabani (botanist of University of Abomey-Calavi, Cotonou, Benin) for plant collections and to Maude Bourlet for her skillful technical assistance. F.V.B. is Maître de Recherches of the Belgian Fonds de la Recherche Scientifique. The authors gratefully thank the Belgian Fonds de la Recherche Scientifique for financial support.

Author information

Authors and Affiliations

  1. Louvain Drug Research Institute, Université catholique de Louvain, Avenue E. Mounier B1.72.03, 1200, Woluwe-Saint-Lambert, Belgium

    Lucy Catteau, Françoise Van Bambeke & Joëlle Quetin-Leclercq

Authors
  1. Lucy Catteau
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Françoise Van Bambeke
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Joëlle Quetin-Leclercq
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lucy Catteau.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Catteau, L., Van Bambeke, F. & Quetin-Leclercq, J. Preliminary evidences of the direct and indirect antimicrobial activity of 12 plants used in traditional medicine in Africa. Phytochem Rev 14, 975–991 (2015). https://doi.org/10.1007/s11101-015-9437-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11101-015-9437-x

Keywords

Search

Navigation