Phytochemistry Reviews

, Volume 9, Issue 2, pp 197–203 | Cite as

Altitudinal variation of secondary metabolites in flowering heads of the Asteraceae: trends and causes

  • Christian ZidornEmail author


Plants in alpine habitats are exposed to severe environmental stresses including temperature and radiation extremes. The observation that flowering heads from high altitude populations of Scorzoneroides helvetica (Mérat) J.Holub (synonym: Leontodon helveticus Mérat) contained higher amounts of luteolin derivatives than conspecific populations in lower altitudes prompted further investigations. An elevational increase of phenolics was confirmed in flowering heads of neophytic populations of three additional taxa of the Cichorieae tribe in New Zealand. A solely genetic basis of the altitudinal trends of flavonoids and phenolic acids was eliminated by field experiments with cultivars of the medicinal herbs Arnica montana L. and Matricaria chamomilla L. planted at nine different altitudes ranging from 600 to 2,200 m in the Tyrolean Alps. Parallel experiments on potted plants excluded soil characteristics as the factor causing the observed variation. The initial hypotheses that enhanced UV-B radiation in higher altitudes was triggering an increase in the ratio of B-ring-ortho-diphenolic versus B-ring-monophenolic flavonols in flowering heads of Arnica was disproved by climate chamber experiments resulting in no significant difference between plants grown in ambient and threefold ambient UV-B radiation regimes. In contrast, an increase of this ratio similar to the changes observed in higher altitudes resulted from a decrease in temperature by 5°C in a second climate chamber experiment. Conclusively, enhanced UV-B radiation is probably not the key factor inducing shifts in the phenolic composition in Asteraceae growing at higher altitudes but it is rather the temperature which decreases with altitude.


Antioxidants Chemical ecology Free radicals Temperature UV-B radiation 



The author is indebted to Serhat Cicek and Renate Spitaler (both Innsbruck) for fruitful discussions and proofreading.


  1. Albert A, Sareedenchai V, Heller W, Seidlitz HK, Zidorn C (2009) Temperature is the key to altitudinal variation of phenolics in alpine plants. Oecologia 160:1–8CrossRefPubMedGoogle Scholar
  2. Alonso-Amelot ME, Oliveros-Bastidas A, Calcagno-Pisarelli MP (2004) Phenolics and condensed tannins in relation to altitude in neotropical Pteridium spp. A field study in the Venezuelan Andes. Biochem Syst Ecol 32:969–981CrossRefGoogle Scholar
  3. Alonso-Amelot ME, Oliveros-Bastidas A, Calcagno-Pisarelli MP (2007) Phenolics and condensed tannins of high altitude Pteridium arachnoideum in relation to sunlight exposure, elevation, and rain regime. Biochem Syst Ecol 35:1–10CrossRefGoogle Scholar
  4. Bakus GJ, Green G (1974) Toxicity in sponges and holothurians: a geographic pattern. Science 185:951–953CrossRefPubMedGoogle Scholar
  5. Bilger W, Rolland M, Nybakken L (2007) UV Screening in higher plants induced by low temperature in the absence of UV-B radiation. Photochem Photobiol Sci 6:190–195CrossRefPubMedGoogle Scholar
  6. Blumthaler M, Ambach W, Ellinger R (1997) Increase in solar UV radiation with altitude. J Photochem Photobiol B 39:130–134CrossRefGoogle Scholar
  7. Bornman JF, Reuber S, Cen YP, Weissenböck G (1997) Ultraviolet radiation as a stress factor and the role of protective pigments. In: Lumsden P (ed) Plants and UV-B: responses to environmental change. Cambridge University Press, Cambridge, pp 157–168Google Scholar
  8. Bruelheide H, Scheidel U (1999) Slug herbivory as a limiting factor for the geographical range of Arnica montana. J Ecol 87:839–848CrossRefGoogle Scholar
  9. Carey DB, Wink M (1994) Elevational variation of quinolizidine alkaloid contents in a lupine (Lupinus argenteus) of the Rocky Mountains. J Chem Ecol 20:849–857CrossRefGoogle Scholar
  10. Ganzera M, Guggenberger M, Stuppner H, Zidorn C (2008) Altitudinal variation of secondary metabolite profiles in flowering heads of Matricaria chamomilla cv. BONA. Planta Med 74:453–457CrossRefPubMedGoogle Scholar
  11. Giménez E, Melendo M, Valle F, Gómez-Mercado F, Cano E (2004) Endemic flora biodiversity in the south of the Iberian Peninsula: altitudinal distribution, life forms and dispersal modes. Biodivers Conserv 13:2641–2660CrossRefGoogle Scholar
  12. Ibdah M, Krins A, Seidlitz HK, Heller W, Strack D, Vogt T (2002) Spectral dependence of flavonol and betacyanin accumulation in Mesembryanthemum crystallinum under enhanced ultraviolet radiation. Plant Cell Environ 25:1145–1154CrossRefGoogle Scholar
  13. Körner C (1999) Alpine plant life. Functional plant ecology of high mountain ecosystems. Springer, BerlinGoogle Scholar
  14. Makepeace W (1981) Polymorphism and the chromosomal number of Hieracium pilosella in New Zealand. N Z J Bot 19:255–258Google Scholar
  15. Malo JE, Baonza J (2002) Are there predictable clines in plant-pollinator interactions along altitudinal gradients? The example of Cytisus scoparius (L.) Link in the Sierra de Guadarrama (Central Spain). Divers Distrib 8:365–371CrossRefGoogle Scholar
  16. Markham KR, Ryan KG, Bloor SJ, Mitchell KA (1998a) An increase in the luteolin : apigenin ratio in Marchantia polymorpha on UV-B enhancement. Phytochemistry 48:791–794CrossRefGoogle Scholar
  17. Markham KR, Tanner GJ, Cassi-Lit M, Whitecross MI, Nayudu M, Mitchell KA (1998b) Possible protective role for 3′, 4′-dihydroxyflavones induced by enhanced UV-B in a UV-tolerant rice cultivar. Phytochemistry 49:1913–1919CrossRefGoogle Scholar
  18. Nagy L, Grabherr G (2009) The biology of Alpine habitats. Oxford University Press, OxfordGoogle Scholar
  19. Rahbek C (1995) The elevational gradient of species richness: a uniform pattern? Ecography 18:200–205CrossRefGoogle Scholar
  20. Rahbek C (1997) The relationship among area, elevation, and regional species richness in neotropical birds. Am Nat 149:875–902CrossRefPubMedGoogle Scholar
  21. Rieger G, Müller M, Guttenberger H, Bucar F (2008) Influence of altitudinal variation on the content of phenolic compounds in wild populations of Calluna vulgaris, Sambucus nigra, and Vaccinium myrtillus. J Agric Food Chem 56:9080–9086CrossRefPubMedGoogle Scholar
  22. Ries G, Heller W, Puchta H, Sandermann H, Seidlitz HK, Hohn B (2000) Elevated UV-B radiation reduces genome stability in plants. Nature 406:98–101CrossRefPubMedGoogle Scholar
  23. Scheidel U, Bruelheide H (2001) Altitudinal differences in herbivory on montane Compositae species. Oecologia 129:75–86CrossRefGoogle Scholar
  24. Scheidel U, Röhl S, Bruelheide H (2003) Altitudinal gradients of generalist and specialist herbivory on three montane Asteraceae. Acta Oecol 24:275–283CrossRefGoogle Scholar
  25. Siska EL, Pennings SC, Buck TL, Hanisak DM (2002) Latitudinal variation in palatability of salt-marsh plants: which traits are responsible? Ecology 83:3369–3381CrossRefGoogle Scholar
  26. Spitaler R, Schlorhaufer PD, Ellmerer EP, Merfort I, Bortenschlager S, Stuppner H, Zidorn C (2006) Altitudinal variation of secondary metabolite profiles in flowering heads of Arnica montana cv. ARBO. Phytochemistry 67:409–417CrossRefPubMedGoogle Scholar
  27. Spitaler R, Winkler A, Lins I, Yanar S, Stuppner H, Zidorn C (2008) Altitudinal variation of phenolic contents in flowering heads of Arnica montana cv. ARBO: a 3-year comparison. J Chem Ecol 34:369–375CrossRefPubMedGoogle Scholar
  28. Vetaas OR, Grytnes J-A (2002) Distribution of vascular plant species richness and endemic richness along the Himalayan elevation gradient in Nepal. Glob Ecol Biogeogr 11:291–301CrossRefGoogle Scholar
  29. Wang G, Zhou G, Yang L, Li Z (2002) Distribution, species diversity and life form spectra of plant communities along an altitudinal gradient in the northern slopes of Qilianshan Mountains, Gansu, China. Plant Ecol 165:169–181CrossRefGoogle Scholar
  30. Xenophontos M, Stavropoulos I, Avramakis E, Navakoudis E, Dornemann D, Kotzabasis K (2008) Influence of the habitat altitude on the (proto)hypericin and (proto)pseudohypericin levels of Hypericum plants from Crete. Planta Med 74:1496–1503CrossRefPubMedGoogle Scholar
  31. Zidorn C, Stuppner H (2001a) Evaluation of chemosystematic characters in the genus Leontodon. Taxon 50:115–133CrossRefGoogle Scholar
  32. Zidorn C, Stuppner H (2001b) Chemosystematics of taxa from the Leontodon section Oporinia. Biochem Syst Ecol 29:827–837CrossRefPubMedGoogle Scholar
  33. Zidorn C, Gottschlich G, Stuppner H (2002) Chemosystematic investigations on phenolics from flowerheads of central european taxa of Hieracium (Asteraceae). Plant Syst Evol 231:39–58CrossRefGoogle Scholar
  34. Zidorn C, Schubert B, Stuppner H (2005) Altitudinal differences in the contents of phenolics in flowering heads of three members of the tribe Lactuceae (Asteraceae) occurring as introduced species in New Zealand. Biochem Syst Ecol 33:855–872CrossRefGoogle Scholar
  35. Zidorn C, Schubert B, Stuppner H (2009) Phenolics as chemosystematic markers in and for the genus Crepis (Asteraceae, Cichorieae). Sci Pharm 76:743–750CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Institut für Pharmazie, Abteilung PharmakognosieUniversität InnsbruckInnsbruckAustria

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