, Volume 56, Issue 4, pp 1039–1046 | Cite as

Effect of ammonium/nitrate ratio on pak choi (Brassica chinensis L.) photosynthetic capacity and biomass accumulation under low light intensity and water deficit

  • H.Q. Shang
  • G.M. Shen
Original paper


We conducted a hydroponic experiment in order to study effects of the ammonium/nitrate ratio (0:15, 5:10, 7.5:7.5, and 10:5) on photosynthetic characteristics and biomass accumulation in Brassica chinensis under low light intensity and water stress. Results showed that net photosynthetic rate, transpiration rate, intrinsic water-use efficiency, stomatal conductance, intercellular CO2 concentration, effective quantum yield of PSII photochemistry, electron transport rate, and nonphotochemical quenching were lower in the treatment (low light intensity and water deficit) than those in the control, whereas stomatal limitation increased. Minimum fluorescence, maximal quantum yield of PSII photochemistry, and photochemical quenching were largely unchanged. Pigment contents first increased and then decreased as the ammonium/nitrate ratios were altered, with significant differences between treatment and control observed at all ratios except for 10:5. Biomass first increased slightly and then decreased both in treated and control plants. Results suggest that economic losses caused by extreme conditions can be minimized by a proper adjustment of the ammonium/nitrate ratio.

Additional key words

chlorophyll fluorescence electron transport rate nitrate reductase NH4+ NO3 stomatal conductance water-use efficiency 







intercellular CO2 concentration


transpiration rate


electron transport rate


maximum fluorescence


minimum fluorescence


fluorescence at stable state


variable fluorescence


maximal quantum yield of PSII


stomatal conductance


stomatal limitation


nonphotochemical quenching


nitrate reductase


polyethylene glycol


net photosynthetic rate


photosynthetic quenching


intrinsic wateruse efficiency (= PN/gs)


effective quantum yield of PSII photochemistry


water potential


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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  1. 1.Key Laboratory of Plant Biology, Department of Life SciencesHeze UniversityHeze, ShandongChina

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