, Volume 56, Issue 4, pp 1123–1133 | Cite as

Comparative chloroplast proteome analysis of exogenously supplied trehalose to wheat seedlings under heat stress

  • Y. Luo
  • H. Y. Liu
  • Y. Z. Fan
  • W. Wang
  • Y. Y. Zhao
Original paper


The aim of our study was to investigate the underlying molecular mechanisms of exogenously supplied trehalose affecting wheat photosynthesis under heat stress. The amount of ATP synthase (ATPase), oxygen-evolving enhancer protein (OEE), PsbP, Rubisco, chloroplast fructose-bisphosphate aldolase (FBPA), and ferredoxin-NADP(H) oxidoreductase (FNR) were downregulated, while PSI reaction center subunits were upregulated under heat stress. However, in the trehalose-pretreated groups, the amount of FNR, cytochrome b6f complex, PSI reaction center subunits, ATPase, FBPA, and Rubisco were upregulated under normal growth conditions and heat stress. Besides, during the recovery period, the upregulation in CAB, PsbP, OEE2, and ATPase suggested that trehalose pretreatment might help to the recovery of PSII and PSI. These results indicate that trehalose pretreatment effectively regulates the levels of the photosynthesis-related proteins and relieves the damage of heat stress to wheat chloroplast.

Additional key words

chlorophyll fluorescence chloroplast photosynthesis-related proteome PSI activity 



chlorophyll a-b binding protein


cyclic electron flow



Cyt b6f

cytochrome b6f complex


electron transfer rate of PSI


electron transfer rate of PSII


minimal fluorescence yield of the dark-adapted state


maximum fluorescence


the maximum quantum yield of PSII photochemistry


flavin adenine dinucleotide


chloroplast fructose-bisphosphate aldolase


fresh mass


ferredoxin-NADP(H) oxidoreductase


isoelectric focusing


mass spectrometry


oxygen-evolving complex


oxygen-evolving enhancer protein


maximal P700 signal


PSI reaction center subunit II


PSI reaction center subunit IV


photochemical quenching coefficient


standard deviation


temperature stress-induced lipocalin


photochemical quantum yield of PSI


effective photochemical quantum yield of PSII


nonphotochemical PSI quantum yield of acceptor-side


nonphotochemical PSI quantum yield of donor-side


PSII quantum yield of nonregulated energy dissipation


PSII quantum yield of regulated energy dissipation


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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  • Y. Luo
    • 1
  • H. Y. Liu
    • 1
  • Y. Z. Fan
    • 1
  • W. Wang
    • 1
  • Y. Y. Zhao
    • 1
  1. 1.Instruments Sharing Platform of School of Life SciencesEast China Normal UniversityShanghaiChina

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