, Volume 56, Issue 4, pp 1171–1176 | Cite as

Thermal acclimation of the temperature dependence of the VCmax of Rubisco in quinoa

  • J.A. Bunce
Original paper


Changes in the temperature dependence of the maximum carboxylation capacity (VCmax) of Rubisco during thermal acclimation of PN remain controversial. I tested for acclimation of the temperature dependence of VCmax in quinoa, wheat, and alfalfa. Plants were grown with day/night temperatures of 12/6, 20/14, and 28/22°C. Responses of PN to substomatal CO2 (Ci) and CO2 at Rubisco (Cc) were measured at leaf temperatures of 10–30°C. VCmax was determined from the initial slope of the PNvs. Ci or Cc curve. Slopes of linear regressions of 1/VCmaxvs. 1/T [K] provided estimates the activation energy. In wheat and alfalfa the increases in activation energy with growth temperature calculated using Ci did not always occur when using Cc, indicating the importance of mesophyll conductance when estimating the activation energy. However, in quinoa, the mean activation energy approximately doubled between the lowest and highest growth temperatures, whether based on Ci or Cc.

Additional key words

carboxylation mesophyll conductance photosynthesis 



[CO2] in the substomatal (intercellular) airspace


[CO2] at Rubisco


mesophyll conductance to CO2


the maximum rate of photosynthetic electron transport


the Michaelis constant of Rubisco carboxylation


net photosynthetic rate


the maximum rate of carboxylation of Rubisco


activation energy


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  1. Barbour M.M., Bachmann S., Bansal U. et al.: Genetic control of mesophyll conductance in common wheat.–New Phytol. 209: 461–465, 2016.CrossRefPubMedGoogle Scholar
  2. Bernacchi C.J., Pimentel C., Long S.P.: In vivo temperature response functions of parameters required to model RuBP-limited photosynthesis.–Plant Cell Environ. 26: 1419–1430, 2003.CrossRefGoogle Scholar
  3. Bunce J.A.: Acclimation of photosynthesis to temperature in eight cool and warm climate herbaceous C3 species: temperature dependence of parameters of a biochemical photosynthesis model.–Photosynth. Res. 63: 59–67, 2000.CrossRefPubMedGoogle Scholar
  4. Bunce J.A.: Effects of elevated carbon dioxide on photosynthesis and productivity of alfalfa in relation to seasonal changes in temperature.–Physiol. Mol. Biol. Plant. 13: 243–252, 2007.Google Scholar
  5. Bunce J.A.: Acclimation of photosynthesis to temperature in Arabidopsis thaliana and Brassica oleracea.–Photosynthetica 46: 517–524, 2008.CrossRefGoogle Scholar
  6. Bunce J.A.: Use of the response of photosynthesis to oxygen to estimate mesophyll conductance to carbon dioxide in waterstressed soybean leaves.–Plant Cell Environ. 32: 875–881, 2009.CrossRefPubMedGoogle Scholar
  7. Busch F.A., Sage R.F.: The sensitivity of photosynthesis to O2 and CO2 concentration identifies strong Rubisco control above the thermal optimum.–New Phytol. 213: 1036–1051, 2017.CrossRefPubMedGoogle Scholar
  8. Cavanagh A.P., Kubien D.S.: Can phenotypic plasticity in Rubisco performance contribute to photosynthetic acclimation?–Photosynth. Res. 119: 203–214, 2014.CrossRefPubMedGoogle Scholar
  9. Farquhar G.D., von Caemmerer S., Berry J.A: A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species.–Planta 149: 78–90, 1980.CrossRefPubMedGoogle Scholar
  10. Galmés J., Hermida-Carrera C., Laanisto L., Niinemets U.A.: Compendium of temperature responses of Rubisco kinetic traits: variability among and within photosynthetic groups and impacts on photosynthesis modeling.–J. Exp. Bot. 67: 5067–5091, 2016.CrossRefPubMedPubMedCentralGoogle Scholar
  11. Hikosaka K., Ishikawa K., Borjigidai A. et al.: Temperature acclimation of photosynthesis: mechanisms involved in the changes in temperature dependence of photosynthetic rate.–J. Exp. Bot. 57: 291–302, 2006.CrossRefPubMedGoogle Scholar
  12. June T., Evans J.R., Farquhar G.D.: A simple new equation for the reversible temperature dependence of photosynthetic electron transport: a study on soybean leaf.–Funct. Plant. Biol. 31: 275–283, 2004.CrossRefGoogle Scholar
  13. Kattge J., Knorr W.: Temperature acclimation in a biochemical model of photosynthesis: a reanalysis of data from 36 species.–Plant Cell Environ. 30: 1176–1190, 2007.CrossRefPubMedGoogle Scholar
  14. Leuning R.: Temperature dependence of two parameters in a photosynthesis model.–Plant Cell Environ. 25: 1205–1210, 2002.CrossRefGoogle Scholar
  15. Medlyn B.E., Dreyer E., Ellsworth D. et al.: Temperature response of parameters of a biochemically based model of photosynthesis. II. A review of experimental data.–Plant Cell Environ. 25: 1167–1179, 2002.CrossRefGoogle Scholar
  16. Onoda Y., Hikosaka K., Hirose K.: The balance between RuBP carboxylation and RuBP regeneration: a mechanism underlying the interspecific variation in acclimation of photosynthesis to seasonal change in temperature.–Funct. Plant. Biol. 32: 903–910, 2005.CrossRefGoogle Scholar
  17. Sage R.F., Kubien D.S.: The temperature response of C3 and C4 photosynthesis.–Plant Cell Environ. 30: 1086–1106, 2007.CrossRefPubMedGoogle Scholar
  18. von Caemmerer S., Evans J.R.: Temperature responses of mesophyll conductance differ greatly between species.–Plant Cell Environ. 38: 629–637, 2015.CrossRefGoogle Scholar
  19. Yamaguchi D.P., Nakaji R., Hiura T., Hikosaka K.: Effects of seasonal change and experimental warming on the temperature dependence of photosynthesis in the canopy leaves of Quercus serrata.–Tree Physiol. 36: 1283–1295, 2016.CrossRefPubMedGoogle Scholar
  20. Yamori W., Noguchi K., Terashima I.: Temperature acclimation of photosynthesis in spinach leaves: analyses of photosynthetic components and temperature dependencies of photosynthetic partial reactions.–Plant Cell Environ. 28: 536–547, 2005.CrossRefGoogle Scholar

Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  1. 1.USDA-ARS, Crop Systems and Global Change LaboratoryBeltsvilleUSA

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