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Photosynthetica

, Volume 56, Issue 4, pp 1140–1146 | Cite as

Physio-anatomical responses of tobacco under caffeine stress

  • R. Alkhatib
  • B. Alkhatib
  • L. Al-Eitan
  • N. Abdo
  • M. Tadros
  • E. Bsoul
Original paper
  • 24 Downloads

Abstract

Caffeine, a purine alkaloid, is reported to act both as an inducer or inhibitor to plant growth in various species. The aim of this study was to examine the effect of exogenous caffeine on tobacco (Nicotiana tabacum) plants, a plant that does not naturally synthesise caffeine. A hydroponic experiment was carried out in a growth chamber for 14 d using Hoagland’s solution supplemented with 0 (control), 25, 50, 100, 1,000; and 5,000 μM caffeine. None of the investigated caffeine concentrations significantly decreased the net photosynthetic rate except the highest concentrations of 1,000 and 5,000 μM. Light microscopy of thick-sectioned roots showed that 1,000 μM and 5,000 μM caffeine-treated plants possessed deformed epidermal cells, reduced number of cortical cells, and deformed vascular tissues with cells exhibiting thickened xylem walls as compared with control plants. Moreover, transmission electron micrographs of roots revealed that mitochondria and the plasma membrane were affected.

Additional key words

chloroplast photosynthetic rate stomatal conductance transmission microscope transpiration rate xylem 

Abbreviations

E

transpiration rate

gs

stomatal conductance

PN

net photosynthetic rate

TEM

transmission electron microscope

SEM

standard errors for the means

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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  • R. Alkhatib
    • 1
  • B. Alkhatib
    • 2
  • L. Al-Eitan
    • 2
  • N. Abdo
    • 3
  • M. Tadros
    • 4
  • E. Bsoul
    • 5
  1. 1.Department of Biotechnology and Genetic Engineering, Faculty of Science and ArtsJordan University of Science and TechnologyIrbidJordan
  2. 2.Department of Applied Biological Sciences, Faculty of Science and ArtsJordan University of Science and TechnologyIrbidJordan
  3. 3.Department of Public Health, Faculty of MedicineJordan University of Science and TechnologyIrbidJordan
  4. 4.Department of Natural Resources and Environment, College of AgricultureJordan University of Science and TechnologyIrbidJordan
  5. 5.Department of Biology and BiotechnologyThe Hashemite UniversityZarqaJordan

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