Abstract
Ultrafast time resolved emission spectra were measured in whole cells of a PSI-deficient mutant of Synechocystis sp. PCC 6803 at room temperature and at 77K to study excitation energy transfer and trapping. By means of a target analysis it was estimated that the terminal emitter of the phycobilisome, termed allophycocyanin 680, transfers its energy with a rate of (20 ps)–1 to PSII. This is faster than the intraphycobilisome energy transfer rates between a rod and a core cylinder, or between the core cylinders.
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28 March 2018
The original article has been corrected. The article is published with Open Access but was missing Open Access information. This has been added.
Abbreviations
- APC:
-
allophycocyanin
- DAS:
-
decay-associated spectrum
- ΔPSI:
-
PSI-deficient mutant of Synechocystis sp. PCC 6803
- EAS:
-
evolution-associated spectrum
- ET:
-
electron transfer
- EET:
-
excitation energy transfer
- FWHM:
-
full width at half maximum
- IRF:
-
instrument response function
- PB:
-
phycobilisome
- PC:
-
phycocyanin
- rms:
-
root mean square
- RP:
-
radical pair
- SAS:
-
species-associated spectrum
- SNR:
-
signal to noise ratio
- SS:
-
steady-state spectra
- SVD:
-
singular value decomposition
- TRES:
-
time resolved emission spectrum
- WT:
-
wild type
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Acknowledgments: The ΔPSI mutant which was originally constructed by Wim Vermaas (Arizona State University, Tempe, Arizona, USA) was kindly provided to us by Christiane Funk (Umeå University, Sweden). This research was performed as part of the BioSolar Cells research program, sponsored by the Dutch Ministry of Economic Affairs. This work is part of the research program of the Netherlands Organization for Scientific Research (NWO, previously FOM). IHMvS and RvG acknowledge financial support of the European Research Council [Advanced Grant proposal 267333 (PHOTPROT) to RvG].
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A correction to this article is available at https://doi.org/10.1007/s11099-018-0797-5
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Acuña, A.M., Van Alphen, P., Van Grondelle, R. et al. The phycobilisome terminal emitter transfers its energy with a rate of (20 ps)–1 to photosystem II. Photosynthetica 56, 265–274 (2018). https://doi.org/10.1007/s11099-018-0779-7
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DOI: https://doi.org/10.1007/s11099-018-0779-7