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Photosynthetica

, Volume 56, Issue 1, pp 229–235 | Cite as

Analysis of photosystem II electron transfer with natural PsbA-variants by redox polymer/protein biophotoelectrochemistry

  • V. Hartmann
  • A. Ruff
  • W. Schuhmann
  • M. Rögner
  • M. M. Nowaczyk
Article

Abstract

Redox polymer/protein biophotoelectrochemistry was used to analyse forward electron transfer of isolated PSII complexes with natural PsbA-variants. PsbA1- or PsbA3-PSII was embedded in a redox hydrogel that allows diffusion-free electron transfer to the electrode surface and thus measurement of an immediate photocurrent response. The initial photocurrent density of the electrode is up to ~2-fold higher with PsbA1-PSII under all tested light conditions, the most prominent under high-light [2,300 μmol(photon) m–2 s–1] illumination with 5 μA cm–2 for PsbA3-PSII and 9.5 μA cm–2 for PsbA1-PSII. This indicates more efficient electron transfer in low-light-adapted PsbA1-PSII. In contrast, the photocurrent decays faster in PsbA1-PSII under all tested light conditions, which suggests increased stability of high-light-adapted PsbA3-PSII. These results confirm and extend previous observations that PsbA3-PSII has increased P680+•/QA–• charge recombination and thus less efficient photon-to-charge conversion, whereas PsbA1-PSII is optimised for efficient electron transfer with limited stability.

Additional key words

biophotovoltaics cyanobacteria D1-protein hydrogel 

Abbreviations

Chl

chlorophyll

Cyt

cytochrome

DCMU

3-(3,4-dichlorophenyl)-1,1-dimethylurea

LED

light emitting diode

MES

2-(N-morpholino)ethanesulfonic acid

P680

reaction centre chlorophylls

PEG-DGE

poly(ethyleneglycol)diglycidylether

PET

photosynthetic electron transfer chain

Phe

pheophytin

POs

redox-hydrogel

Q

plastoquinone

ROS

reactive oxygen species

β-DM

N-dodecyl-β-D-maltoside

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Supplementary material

11099_2018_775_MOESM1_ESM.pdf (247 kb)
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11099_2018_775_MOESM2_ESM.pdf (217 kb)
Supplementary material, approximately 216 KB.

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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  • V. Hartmann
    • 1
  • A. Ruff
    • 2
  • W. Schuhmann
    • 2
  • M. Rögner
    • 1
  • M. M. Nowaczyk
    • 1
  1. 1.Plant Biochemistry, Faculty of Biology and BiotechnologyRuhr-University BochumBochumGermany
  2. 2.Analytical Chemistry–Center for Electrochemical Science (CES), Faculty of Chemistry and BiochemistryRuhr-University BochumBochumGermany

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