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Photosynthetica

, Volume 56, Issue 1, pp 150–162 | Cite as

Control of the maximal chlorophyll fluorescence yield by the QB binding site

  • O. Prášil
  • Z. S. Kolber
  • P. G. Falkowski
Article
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Abstract

Differences in maximal yields of chlorophyll variable fluorescence (Fm) induced by single turnover (ST) and multiple turnover (MT) excitation are as great as 40%. Using mutants of Chlamydomonas reinhardtii we investigated potential mechanisms controlling Fm above and beyond the QA redox level. Fm was low when the QB binding site was occupied by PQ and high when the QB binding site was empty or occupied by a PSII herbicide. Furthermore, in mutants with impaired rates of plastoquinol reoxidation, Fm was reached rapidly during MT excitation. In PSII particles with no mobile PQ pool, Fm was virtually identical to that obtained in the presence of PSII herbicides. We have developed a model to account for the variations in maximal fluorescence yields based on the occupancy of the QB binding site. The model predicts that the variations in maximal fluorescence yields are caused by the capacity of secondary electron acceptors to reoxidize QA–.

Additional key words

conformational change electron transport photosystem II thylakoid membrane 

Abbreviations

2,5 DMBQ

2,5-dimethyl-p-benzoquinone

2,6 DCBQ

2,6-dichloro-p-benzoquinone

Chl

chlorophyll

DBMIB

2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone

DCMU

3-(3,4,-dichlorophenyl)-1,1-dimethylurea

FRR

fast repetition rate

F0

initial fluorescence yield in dark-adapted sample

Fi

initial fluorescence yield in light or following preillumination

Fm

maximal fluorescence yield

HF1

Fm induced by first ST excitation in dark-adapted cells

HFD

Fm in the presence of saturating amounts of PSII herbicides

HFM

Fm induced by MT excitation

LF

Fm induced by the second ST excitation

MT

multiple turnover

PQ

plastoquinone-9

RCII

reaction center of PSII

ST

single turnover

TL

thermoluminescence

σPSII

functional cross-section of PSII

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© The Institute of Experimental Botany 2018

Authors and Affiliations

  1. 1.Institute of Microbiology CASCenter AlgatechTřeboňCzech Republic
  2. 2.Soliense Inc.ShorehamUSA
  3. 3.Environmental Biophysics and Molecular Ecology Program, Departments of Marine and Coastal Science, and Earth and Planetary ScienceRutgers UniversityNew BrunswickUSA

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