Control of the maximal chlorophyll fluorescence yield by the QB binding site
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Abstract
Differences in maximal yields of chlorophyll variable fluorescence (Fm) induced by single turnover (ST) and multiple turnover (MT) excitation are as great as 40%. Using mutants of Chlamydomonas reinhardtii we investigated potential mechanisms controlling Fm above and beyond the QA redox level. Fm was low when the QB binding site was occupied by PQ and high when the QB binding site was empty or occupied by a PSII herbicide. Furthermore, in mutants with impaired rates of plastoquinol reoxidation, Fm was reached rapidly during MT excitation. In PSII particles with no mobile PQ pool, Fm was virtually identical to that obtained in the presence of PSII herbicides. We have developed a model to account for the variations in maximal fluorescence yields based on the occupancy of the QB binding site. The model predicts that the variations in maximal fluorescence yields are caused by the capacity of secondary electron acceptors to reoxidize QA–.
Additional key words
conformational change electron transport photosystem II thylakoid membraneAbbreviations
- 2,5 DMBQ
2,5-dimethyl-p-benzoquinone
- 2,6 DCBQ
2,6-dichloro-p-benzoquinone
- Chl
chlorophyll
- DBMIB
2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone
- DCMU
3-(3,4,-dichlorophenyl)-1,1-dimethylurea
- FRR
fast repetition rate
- F0
initial fluorescence yield in dark-adapted sample
- Fi
initial fluorescence yield in light or following preillumination
- Fm
maximal fluorescence yield
- HF1
Fm induced by first ST excitation in dark-adapted cells
- HFD
Fm in the presence of saturating amounts of PSII herbicides
- HFM
Fm induced by MT excitation
- LF
Fm induced by the second ST excitation
- MT
multiple turnover
- PQ
plastoquinone-9
- RCII
reaction center of PSII
- ST
single turnover
- TL
thermoluminescence
- σPSII
functional cross-section of PSII
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References
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