, Volume 56, Issue 1, pp 427–432 | Cite as

Susceptibility of an ascorbate-deficient mutant of Arabidopsis to high-light stress

  • L.-D. Zeng
  • M. Li
  • W. S. Chow
  • C.-L. Peng


Ascorbate is an important antioxidant involved in both enzymatic and nonenzymatic reactions in plant cells. To reveal the function of ascorbate associated with defense against photo-oxidative damage, responses of the ascorbate-deficient mutant vtc2-1 of Arabidopsis thaliana to high-light stress were investigated. After high-light treatment at 1,600 μmol(photon) m–2 s–1 for 8 h, the vtc2-1 mutant exhibited visible photo-oxidative damage. The total ascorbate content was lower, whereas accumulation of H2O2 was higher in the vtc2-1 mutant than that in the wild type. The chlorophyll (Chl) content and PSII Chl fluorescence parameters, such as maximal quantum yield of PSII photochemistry, yield, and electron transport rate, in vtc2-1 mutant decreased more than that in the wild type, whereas the nonphotochemical quenching coefficient increased more in the wild type than that in vtc2-1 mutant. Therefore, the vtc2-1 mutant was more sensitive to high-light stress than the wild type. Accumulation of reactive oxygen species was mainly responsible for the damage of PSII in the vtc2-1 mutant under high light. The results indicate that ascorbate plays a critical role in maintaining normal photosynthetic function in plants under high-light stress.

Additional key words

Arabidopsis thaliana ascorbic acid high-light stress chlorophyll fluorescence reactive oxygen species 



ascorbate peroxidase


ascorbic acid

AsA-GSH cycle

ascorbate–glutathione cycle








electron transport rate


minimal fluorescence yield of the dark-adapted state


maximal fluorescence yield of the dark-adapted state


maximal fluorescence yield of the light-adapted state


steady-state fluorescence yield


variable fluorescence


maximal quantum yield of PSII photochemistry


nonphotochemical quenching


open reading frame


nonphotochemical quenching coefficient


photochemical quenching coefficient


relative humidity


reactive oxygen species


superoxide dismutase


trichloroacetic acid


effective quantum yield of PSII photochemistry.


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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  1. 1.Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitor, Guangdong Provincial Key Laboratory of Biotechnology for Plant DevelopmentCollege of Life Science, South China Normal UniversityGuangzhouChina
  2. 2.School of Life ScienceHuizhou UniversityHuizhou City, Guangdong ProvinceChina
  3. 3.Division of Plant Science, Research School of Biology, College of Medicine, Biology and EnvironmentThe Australian National University, ActonAustralian Capital TerritoryAustralia

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