Photosynthetica

, Volume 56, Issue 1, pp 236–243 | Cite as

Influence of the disaccharide trehalose on the oxidizing side of photosystem II

  • M. D. Mamedov
  • E. S. Nosikova
  • L. A. Vitukhnovskaya
  • A. A. Zaspa
  • A. Yu. Semenov
Article
  • 34 Downloads

Abstract

The steady-state oxygen evolution rate was previously shown to be stimulated by the disaccharide trehalose in PSII suspension. Here we showed a similar increase in the rate of oxygen evolution in PSII core complexes from spinach in solution and in proteoliposomes in the presence of trehalose. Using direct electrometrical technique, we also revealed that trehalose had no effect on the kinetics of electron transfer from Mn to redox-active-tyrosyl radical, YZ (S1 → S2 transition), while it accelerated the kinetics of electrogenic proton transport during S2 → S3 and S4 → S0 transitions of the wateroxidizing complex (WOC) induced by the first, second, and third laser flashes in dark-adapted PSII samples. These observations imply that the effect of trehalose occurrs due to its interaction with the WOC.

Additional key words

effective functioning osmolyte photoelectric response vectorial transfer 

Abbreviations

Chl

chlorophyll

P680

the primary electron donor of PSII

QA, QB

the primary and the secondary plastoquinone electron acceptors of PSII, respectively

RC

reaction center

S0-S4

charge storage states of the WOC

WOC

water-oxidizing complex

YZ

redox active tyrosine residue 161 of D1 protein

Δψ

transmembrane electric potential difference

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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  • M. D. Mamedov
    • 1
  • E. S. Nosikova
    • 2
  • L. A. Vitukhnovskaya
    • 1
  • A. A. Zaspa
    • 1
  • A. Yu. Semenov
    • 1
  1. 1.Belozersky Institute of Physical-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Faculty of Bioengineering and BioinformaticsLomonosov Moscow State UniversityMoscowRussia

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