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Virus-induced changes in photosynthetic parameters and peroxidase isoenzyme contents in tomato (Solanum lycopersicum L.) plants

Photosynthetica

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Abstract

Tomato samples were collected from the field of Absheron peninsula in Azerbaijan in order to evaluate the incidence of main Tobamoviruses. According to results of serological and molecular tests, Tomato mosaic virus (ToMV), Tobacco mosaic virus (TMV), and Pepper mild mottle virus (PMMoV) were detected as single and mixed infections (TMV + PMMoV; ToMV + PMMoV) in various tomato samples. It was found that Tobamovirus infection caused an increase in the content of malondialdehyde, alterations in the activities of peroxidase enzymes and quantitative and qualitative changes in their molecular isoforms. A comparison of thylakoid membrane polypeptides from virus-infected leaves indicated a decrease in the content of the thylakoid membrane polypeptides with molecular masses of 123, 55, 47, 33, 28–24, 17, and 15 kD. PSII efficiency and the content of chlorophylls (a and b) were significantly lower in the virus-infected leaves.

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Abbreviations

APO:

ascorbate peroxidase

BPO:

benzidine-type peroxidase

Chl:

chlorophyll

CMV:

Cucumber mosaic virus

F0 :

initial values of chlorophyll fluorescence

Fm :

maximal values of chlorophyll fluorescence

Fv/Fm :

maximal quantum efficiency of PSII

GPO:

guaiacol-type peroxidase

LPO:

lipid peroxidation

PMMoV:

Pepper mild mottle virus

ROS:

reactive oxygen species

TMV:

Tobacco mosaic virus

ToMV:

Tomato mosaic virus

TSWV:

Tomato spotted wilt virus

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Authors and Affiliations

  1. Institute of Molecular Biology and Biotechnology, Azerbaijan National Academy of Sciences, 2A Matbuat Avenue, Baku, AZ1073, Azerbaijan

    I. M. Huseynova, S. M. Mirzayeva, N. F. Sultanova, D. R. Aliyeva, N. Sh. Mustafayev & J. A. Aliyev

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  1. I. M. Huseynova
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  2. S. M. Mirzayeva
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  3. N. F. Sultanova
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  4. D. R. Aliyeva
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  5. N. Sh. Mustafayev
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  6. J. A. Aliyev
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Correspondence to I. M. Huseynova.

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Acknowledgment: This work was supported by the Science Development Foundation under the President of the Republic of Azerbaijan — Grant № EIF-2014-9(24)-KETPL-14/11/3 and Grant № EIF/GAM-3-2014-6(21)-24/15/3.

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Huseynova, I.M., Mirzayeva, S.M., Sultanova, N.F. et al. Virus-induced changes in photosynthetic parameters and peroxidase isoenzyme contents in tomato (Solanum lycopersicum L.) plants. Photosynthetica 56, 841–850 (2018). https://doi.org/10.1007/s11099-017-0737-9

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  • DOI: https://doi.org/10.1007/s11099-017-0737-9

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