The objective of this study was to evaluate the response of the giant reed (Arundo donax L.) to drought stress at early stages, as well as to determine the effects of limited soil water availability on plant growth, gas exchange, and water-use efficiency. Plantlets of a commercial clone were grown in a greenhouse under two water treatments: at 100% of field capacity and progressive drought for 66 days (until 20% of field capacity). Soil water content, leaf elongation rate, plant water consumption, and gas-exchange parameters were measured throughout the experiment. Total plant biomass, leaf water, and osmotic potential were determined at the end of the experiment. Plant growth and leaf gas-exchange parameters were significantly affected by soil water availability, but only when it was below 40% of field capacity. At early stages, Arundo donax showed drought stress acclimation due to leaf plasticity, stomatal regulation, and osmotic adjustment.
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- C c :
chloroplastic CO2 concentration
- C i :
substomatal CO2 concentration
days after transplantation
field capacity percentage
- g s :
- g m :
- H2Oc :
total water consumption
- J max :
the potential light- saturated electron transport rate
- J flu :
the electron transport rate
- P N :
net photosynthetic rate
- R D :
respiration rate in the light
- R n :
the respiratory rate in the absence of light
- V cmax :
the maximum Rubisco carboxylation rate
Angelini L.G., Ceccarini L., Bonari E.: Biomass yield and energy balance of giant reed (Arundo donax L.) cropped in central Italy as related to different management practices. — Eur. J. Agron. 22: 375–389, 2005.
Anjum S., Xie X., Wang L.: Morphological, physiological and biochemical responses of plants to drought stress. — Afr. J. Agr. Res. 6: 2026–2032, 2011.
Arcidiacono C., Porto S.M.C.: Life cycle assessment of Arundo donax biomass production in a mediterranean experimental field using treated wastewater. — J. Agric. Eng. 42: 29–38, 2012.
Babu R.C., Pathan M.S., Blum A., Nguyen H.T.: Comparison of measurement methods of osmotic adjustment in rice cultivars. — Crop Sci. 39: 150–158, 1999.
Bell G.P.: Ecology and management of Arundo donax, and approaches to riparian habitat restoration in southern California. — In: Brock J (ed.): Plant Invasions: Studies from North America and Europe. Pp. 103–113. Backhuys, Leiden 1997.
Bernacchi C.J., Portis A.R., Nakano H. et al.: Temperature response of mesophyll conductance. Implications for the determination of rubisco enzyme kinetics and for limitations to photosynthesis in vivo. — Plant Physiol. 130: 1992–1998, 2002.
Condon A.G., Richards R.A., Rebetzke G.J., Farquhar G.D.: Improving intrinsic water-use efficiency and crop yield. — Crop Sci. 42: 122–131, 2002.
Fernández R.J., Reynolds J.F.: Potential growth and drought tolerance of eight desert grasses: lack of a trade-off? — Oecologia 123: 90–98, 2000.
Flexas J., Bota J., Cifre J. et al.: Understanding down-regulation of photosynthesis under water stress: future prospects and searching for physiological tools for irrigation management. — Ann. Appl. Biol. 144: 273–283, 2004.
Flexas J., Diaz-Espejo A., Galmés J., et al.: Rapid variations of mesophyll conductance in response to changes in CO2 concentration around leaves. — Plant Cell Environ. 30: 1284–1298, 2007.
French R.J., Schultz J.E.: Water use efficiency of wheat in a Mediterranean-type environment. The relation between yield, water use and climate. — Aust. J. Agr. Res. 35: 743–764, 1984.
Galmés J., Medrano H., Flexas J.: Photosynthetic limitations in response to water stress and recovery in Mediterranean plants with different growth forms. — New Phytol. 175: 81–93, 2007.
Grassi G., Magnani F.: Stomatal, mesophyll conductance and biochemical limitations to photosynthesis as affected by drought and leaf ontogeny in ash and oak trees. — Plant Cell Environ. 28: 834–849, 2005.
Gulías J., Cifre J., Jonasson S. et al.: Seasonal and inter-annual variations of gas exchange in thirteen woody species along a climatic gradient in the Mediterranean island of Mallorca. — Flora 204: 169–181, 2009.
Harley P.C., Thomas R.B., Reynolds J.F., Strain B.R.: Modelling photosynthesis of cotton grown in elevated CO2. — Plant Cell Environ. 15: 271–282, 1992.
Haworth M., Centritto M., Giovannelli A. et al.: Xylem morphology determines the drought response of two Arundo donax ecotypes from contrasting habitats. — GCB Bioenergy. 9: 119–131, 2017.
Hsiao T.C., Acevedo E., Fereres E., Henderson D.W.: Water stress, growth, and osmotic adjustment. — Philos. T. R. Soc. B 273: 479–500, 1976.
Kang S., Post W.M., Nichols J.A. et al.: Marginal lands: concept, assessment and management. — J. Agr. Sci. 5: 129–139, 2013.
Lambert A., Dudley T., Saltonstall K.: Ecology and impacts of the large-statured invasive grasses Arundo donax and Phragmites australis in North America. — Invas. Plant Sci. Mana. 3: 489–494, 2010.
Lewandowski I., Scurlock J.M.O., Lindvall E., Christou M.: The development and current status of perennial rhizomatous grasses as energy crops in the US and Europe. — Biomass Bioenerg. 25: 335–361, 2003.
Liu F., Stützel H.: Biomass partitioning, specific leaf area, and water use efficiency of vegetable amaranth (Amaranthus spp.) in response to drought stress. — Sci. Hortic.-Amsterdam 102: 15–27, 2004.
Mann J.J., Kyser G.B., Barney J.N., DiTomaso J.M.: Assessment of aboveground and belowground vegetative fragments as propagules in the bioenergy crops Arundo donax and Miscanthus x giganteus. — Bioenerg. Res. 6: 688–698, 2013.
Medrano H., Escalona J.M., Bota J. et al.: Regulation of photosynthesis of C3 plants in response to progressive drought: stomatal conductance as a reference parameter. — Ann. Bot.-London 89: 895–905, 2002
Meier I.C., Leuschner C.: Leaf size and leaf area index in Fagus sylvatica forests: competing effects of precipitation, temperature, and nitrogen availability. — Ecosystems 11: 655–669, 2008.
Morgan J.M.: Osmoregulation and water stress in higher plants. — Annu. Rev. Plant Physio. 35: 299–319, 1984.
Niinemets U., Cescatti A., Rodeghiero M., Tosens T.: Complex adjustments of photosynthetic potentials and internal diffusion conductance to current and previous light availabilities and leaf age in Mediterranean evergreen species Quercus ilex. — Plant Cell Environ. 29: 1159–1178, 2006.
Nobel P.S.: Physicochemical and Environmental Plant Physiology, 3rd ed. Pp. 540. Academic Press, Oxford 2009.
Paredes D., Trigo R.M., Garcia-Herrera R., Trigo I.F.: Understanding precipitation changes in Iberia in early spring: Weather typing and storm-tracking approaches. — J. Hydrometeorol. 7: 101–113, 2006.
Parry M., Flexas J., Medrano H.: Prospects for crop production under drought: research priorities and future directions. — Ann. Appl. Biol. 147: 211–226, 2005.
Peguero-Pina J.J., Flexas J., Galmés J. et al.: Leaf anatomical properties in relation to differences in mesophyll conductance to CO2 and photosynthesis in two related Mediterranean Abies species. — Plant Cell Environ. 35: 2121–2129, 2012.
Perdue R.E.: Arundo donax. — Source of musical reeds and industrial cellulose. — Econ. Bot. 12: 368–404, 1958.
Pilu R., Badone F., Michela L.: Giant reed (Arundo donax L.): A weed plant or a promising energy crop? — Afr. J. Biotechnol. 11: 9163–9174, 2012.
Pilu R., Manca A., Landoni M.: Arundo donax as an energy crop: Pros and cons of the utilization of this perennial plant. — Maydica 58: 54–59, 2013.
Poorter H., Remkes C.: Leaf area ratio and net assimilation rate of 24 wild species differing in relative growth rate. — Oecologia 83: 553–559, 1990.
Pou A., Flexas J., Alsina M.M. et al: Adjustments of water use efficiency by stomatal regulation during drought and recovery in the drought-adapted Vitis hybrid Richter-110 (V. berlandieri × V. rupestris). — Physiol. Plantarum 134: 313–323, 2008.
Reich P.B., Walters M.B., Ellsworth D.S.: From tropics to tundra: global convergence in plant functioning. — P. Natl. Acad. Sci. USA 94: 13730–13734, 1997.
Sánchez E., Gil S., Azcón-Bieto J., Nogués S.: The response of Arundo donax L. (C3) and Panicum virgatum (C4) to different stresses. — Biomass Bioenerg. 85: 335–345, 2016.
Sánchez E., Scordia D., Lino G. et al.: Salinity and water stress effects on biomass production in different Arundo donax L. clones. — Bioenergy Res. 8: 1461–1479, 2015.
Scordia D., Cosentino S.L., Lee J.W., Jeffries T.W.: Bioconversion of giant reed (Arundo donax L.) hemicellulose hydrolysate to ethanol by Scheffersomyces stipitis CBS6054. — Biomass Bioenerg. 39: 296–305, 2012.
Shortall O.K.: “Marginal land” for energy crops: Exploring definitions and embedded assumptions. — Energ. Policy 62: 19–27, 2013.
Stocker T.F., Qin D., Plattner G.K. et al.: Summary for policymakers. — In: IPCC: The Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge, 2013.
Tomás M., Medrano H., Pou A. et al.: Water-use efficiency in grapevine cultivars grown under controlled conditions: Effects of water stress at the leaf and whole-plant level. — Aust. J. Grape Wine R. 18: 164–172, 2012.
Villagra P.E., Cavagnaro J.B.: Water stress effects on the seedling growth of Prosopis argentina and Prosopis alpataco. — J. Arid. Environ. 64: 390–400, 2006.
Webster R.J., Driever S.M., Kromdijk J. et al.: High C3 photosynthetic capacity and high intrinsic water use efficiency underlies the high productivity of the bioenergy grass Arundo donax. — Sci. Rep. 6: 20694, 2016.
Wright I.J., Westoby M.: Understanding seedling growth relationships through specific leaf area and leaf nitrogen concentration: generalizations across growth forms and growth irradiance. — Oecologia 127: 21–29, 2001.
Wright P., Morgan J.M., Jessop R.S.: Turgor maintenance by osmoregulation in Brassica napus and B. juncea under field conditions. — Ann. Bot.-London 80: 313–319, 1997.
Wu F., Bao W., Li F., Wu N.: Effects of drought stress and N supply on the growth, biomass partitioning and water-use efficiency of Sophora davidii seedlings. — Environ. Exp. Bot. 63: 248–255, 2008.
Acknowledgements: This work was financed by the OPTIMA-FP7. A. Romero-Munar was funded by a fellowship of the Government of Balearic Islands and European Social Fund. The authors would like to thank Mr. Miquel Truyols and collaborators of the UIB Experimental Field (UIB Grant 15/2015) for their support to our experiments.
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Romero-Munar, A., Baraza, E., Cifre, J. et al. Leaf plasticity and stomatal regulation determines the ability of Arundo donax plantlets to cope with water stress. Photosynthetica 56, 698–706 (2018). https://doi.org/10.1007/s11099-017-0719-y
Additional key words
- early stage
- osmotic potential
- stomatal conductance
- water deficit