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Leaf anatomy and photosynthetic efficiency of Acrostichum danaeifolium after UV radiation

  • Original Paper
  • Published:
Photosynthetica

Abstract

This paper reports effects of ultraviolet B (UVB) radiation on leaf anatomy and contents of chlorophyll and carotenoids, as well as photosynthetic parameters, in young sporophytes of Acrostichum danaeifolium Langsd. & Fisch. (Polypodiopsida, Pteridaceae) exposed to UV radiation treatments for 1 h daily for six weeks. The leaves showed large aerenchyma and present chloroplasts in both epidermises. After cultivation under PAR + UVA + UVB, leaves showed curling and malformed stomata on the abaxial face. After the UV treatment, chloroplasts in leaves were arranged against the inner wall of the epidermal cells. Transmission electron microscopy analysis showed some dilated thylakoids and plastoglobuli in chloroplasts and vesicles containing phenolic compounds in the cytoplasm. Differences were not observed between control and UV-treated plants in their contents of chlorophylls, carotenoids, and photosynthetic parameters. A. danaeifolium grown in sunny mangrove environment seems to have mechanisms preventing photosystem damage.

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Abbreviations

A:

absorbance

AL:

actinic light

AT-O:

toluidine blue

Chl:

chlorophyll

DAC:

days of cultivation

Ek:

saturating irradiance

ETR:

electron transport rate

ETRmax :

maximum electron transport rate

Fm':

maximum fluorescence of a light-adapted leaf

Fs :

steady state fluorescence obtained at light-acclimated samples

FII :

ratio of chlorophyll a associated with PSII

FM:

fresh mass

LM:

light microscopy

PAM:

pulse-amplitude modulation

RLC:

rapid light curves

SEM:

scanning electron microscopy

SL:

saturating light pulses

SP:

single saturating pulse

ФPSII :

actual photochemical efficiency of PSII

TEM:

transmission electron microscopy

UV:

ultraviolet

UVA:

ultraviolet A radiation

UVB:

ultraviolet B radiation

Y(II) :

effective quantum yield

αETR :

photosynthetic efficiency

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Acknowledgments

The first author thanks CAPES (Coordination for Improvement of University Staff, Brazil) for the grant and the LCME (Central Laboratory of Electronic Microscopy), UFSC, for the electron microscopy analyses.

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Authors and Affiliations

  1. Department of Botany, Federal University of Santa Catarina, Campus Universitário Reitor João David Ferreira Lima, Trindade, 88040-970, Florianópolis, SC, Brazil

    A. M. Fonini, J. B. Barufi, A. C. Rodrigues & Á. M. Randi

  2. Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina 88049-900, CP 476, Florianópolis, SC, Brazil

    É. C. Schmidt

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  1. A. M. Fonini
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  3. É. C. Schmidt
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  5. Á. M. Randi
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Correspondence to Á. M. Randi.

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Fonini, A.M., Barufi, J.B., Schmidt, É.C. et al. Leaf anatomy and photosynthetic efficiency of Acrostichum danaeifolium after UV radiation. Photosynthetica 55, 401–410 (2017). https://doi.org/10.1007/s11099-016-0654-3

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  • DOI: https://doi.org/10.1007/s11099-016-0654-3

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