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The analysis of determining factors and evaluation of tolerance to photoinhibition in wheat (Triticum aestivum L.)

  • Published:
Photosynthetica

Abstract

Photoinhibition is a significant constraint for improvement of radiation-use efficiency and yield potential in cereal crops. In this work, attached fully expanded leaves of seedlings were used to assay the factors determining photoinhibition and for evaluation of tolerance to photoinhibition in wheat (Triticum aestivum L.). Our results showed that even 1 h under PPFD of 600 µmol(photon) m−2 s−1 could significantly reduce maximal quantum yield of PSII photochemistry (Fv/Fm) and performance index (PI) compared to low light [300 µmol(photon) m−2 s−1]. The decrease of Fv/Fm and PI was more noticeable with the increase of light intensity; irradiance higher than 800 µmol(photon) m−2 s−1 resulted in photoinhibition. Compared to 25°C, lower (20°C) or higher temperature (≥ 35°C) aggravated photoinhibition, while slightly high temperature (28°) alleviated photoinhibition. At 25°C, irradiance of 1,000 µmol(photon) m–2 s–1 for 1 h was enough to cause photoinhibition and a significant decrease of Fv/Fm, PI, trapped energy flux, electron transport flux, and density of reaction center as well as increase of dissipated energy flux per cross section were observed. In addition, seedlings at 21–32 days after planting showed a relatively stable phenotype, while the younger or older seedlings indicated an increased susceptibility to photoinhibition, especially in senescing leaves. Finally, six wheat varieties with relative tolerance to photoinhibition were identified from 22 Chinese winter wheat varieties by exposing attached leaves of the 25-d old seedlings for 1 h to 1,000 µmol(photon) m–2 s–1 at 25°C. Therefore, our work established a possible method for development of new wheat varieties with enhanced tolerance to photoinhibition.

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Abbreviations

Chl:

chlorophyll

DAP:

days after planting

DIo/CS:

dissipated energy flux per cross section

ETo/CS:

electron transport flux per cross section

Fv/Fm :

maximal quantum yield of PSII photochemistry

HL:

high light

LL:

low light

PI:

performance index

PIABS :

performance index on an absorption basis

RC/CSm :

density of reaction center per excited cross section

RUE:

radiation-use efficiency

TRo/CS:

trapped energy flux per cross section

VJ :

relative variable fluorescence intensity at the J-step.

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Authors and Affiliations

  1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    H. Li, Q. Zheng, B. Li & Z. Li

  2. Zhaoxian Institute of Agricultural Sciences, Shijiazhuang, 051530, China

    J. Zhang

Authors
  1. H. Li
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  2. Q. Zheng
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  3. J. Zhang
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  4. B. Li
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  5. Z. Li
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Correspondence to Z. Li.

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Acknowledgements

This work was financially supported by the National Key Basic Research Program (No. 2015CB150106) and the Natural Science Foundation of China (31371609, 31571644).

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Li, H., Zheng, Q., Zhang, J. et al. The analysis of determining factors and evaluation of tolerance to photoinhibition in wheat (Triticum aestivum L.). Photosynthetica 55, 69–76 (2017). https://doi.org/10.1007/s11099-016-0228-4

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  • DOI: https://doi.org/10.1007/s11099-016-0228-4

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