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A marine phytoplankton Prymnesium parvum upregulates the component proteins of photosystem II under iron stress

  • Original Papers
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Photosynthetica

Abstract

Iron is an essential limiting factor for primary production in many marine systems. The present study investigated differential regulation of protein expression in marine phytoplankton Prymnesium parvum under low Fe concentration. The phytoplankton was grown in f/2 culture medium in artificial seawater with low (0.0025 μM) and high (0.05 μM) Fe concentrations. Two-dimensional differential gel electrophoresis and matrix-assisted laser desorption-ionization-time of flight-mass spectrometer analysis were performed for protein identification and characterization. The growth of the alga declined substantially under the low Fe compared to the high Fe concentration. Under low Fe conditions, P. parvum upregulated 10 proteins including chloroplastic ATP synthase subunit b, D2 protein of PSII, D1 protein of PSII reaction centre, and light harvesting complex II protein, most of which are associated with photosynthetic activities in PSII. The results suggest that the marine alga P. parvum altered the biosynthesis of several photosynthetic proteins in order to cope with low Fe conditions.

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Abbreviations

2-D DIGE:

2-dimensional differential gel electrophoresis

Chl:

chlorophyll

HF:

high Fe concentration

IEF:

isoelectric focusing

LF:

low Fe concentration

MALDI-TOF-MS:

matrix-assisted laser desorption-ionization-time of flight-mass spectrometer

NCBI:

National Center for Biotechnology Information

OD:

optical density

OEE:

oxygen-evolving enhancer

PBS:

phosphate buffer saline

PQ:

plastoquinon

ROS:

reactive oxygen species

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Authors and Affiliations

  1. Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa, 920-1192, Japan

    M. M. Rahman

  2. Bangladesh Rice Research Institute, Gazipur, 1701, Dhaka, Bangladesh

    M. M. Rahman

  3. Centre for Environmental Sustainability, School of the Environment, University of Technology Sydney, PO Box 123, Broadway, NSW, 2007, Australia

    M. A. Rahman

  4. Institute of Science and Engineering, Kanazawa University, Kakuma, Kanazawa, 920-1192, Japan

    T. Maki & H. Hasegawa

  5. Division of Functional Genomics, Advanced Science Research Center, Kanazawa University, Takaramachi, Kanazawa, 920-0934, Japan

    T. Nishiuchi & T. Asano

Authors
  1. M. M. Rahman
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  2. M. A. Rahman
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  3. T. Maki
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  4. T. Nishiuchi
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  5. T. Asano
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  6. H. Hasegawa
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Corresponding authors

Correspondence to M. M. Rahman or H. Hasegawa.

Additional information

Acknowledgements: This research was financially supported by a Grant-in-Aid (K2319) for scientific research from the Ministry of the Environment, Japan. The authors would like to thank Dr. Luigi De Filippis, School of the Environmental, University Technology Sydney (UTS), Australia, for reviewing this manuscript prior to submission. The authors highly appreciate the editor and the anonymous reviewers for their comments and suggestions that improved the quality of the paper substantially.

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Rahman, M.M., Rahman, M.A., Maki, T. et al. A marine phytoplankton Prymnesium parvum upregulates the component proteins of photosystem II under iron stress. Photosynthetica 53, 136–143 (2015). https://doi.org/10.1007/s11099-015-0095-4

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  • DOI: https://doi.org/10.1007/s11099-015-0095-4

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