, Volume 52, Issue 4, pp 519–528 | Cite as

Impact of elevated CO2 on growth, physiology, yield, and quality of tomato (Lycopersicon esculentum Mill) cv. Arka Ashish

  • H. Mamatha
  • N. K. Srinivasa RaoEmail author
  • R. H. Laxman
  • K. S. Shivashankara
  • R. M. Bhatt
  • K. C. Pavithra
Original Papers


Tomato meets the dietary nutrient and antioxidant requirements of diverse populations. Being a C3 crop and an important vegetable, it is likely to be influenced by increased CO2 concentrations under climate change situation. This study was conducted to investigate the effects of elevated CO2 on overall physiology, water relations, growth, yield, and fruit quality of tomato (Lycopersicon esculentum Mill) cv. Arka Ashish. Plants were grown at elevated CO2 [550 (EC550) and 700 (EC700) ppm of CO2] in open top chambers. Increased assimilation rate, decreased stomatal conductance and transpiration rate were observed at elevated CO2 (EC) concentrations. Reduced leaf osmotic potential and increased water potential were observed at EC compared with the control (380 ppm of CO2) in flowering and fruiting stages. Lower total chlorophyll content was recorded at EC700. Plant height was significantly higher at EC550 compared with EC700. Higher number of branches was observed at EC700 as compared with plants grown at EC550 and the control. Leaf area was lower at EC700 compared with EC550 but specific leaf mass was higher at EC700. Due to higher leaf dry mass and root dry mass, the plants grown at EC700 exhibited higher total dry mass compared to EC550 and the control. Increased number of flowers and fruits together with higher fruit set led to higher fruit yield at both EC concentrations. The highest yield increase was observed at EC700. The fruits showed a lower content of phenols, flavonoids, ferric reducing antioxidant potential, total soluble solids, and titratable acidity in plants grown at EC as compared with the control. The ascorbic acid content was high at both EC700 and EC550. Carotenoids and lycopene content was low at EC700 compared to higher content observed at EC550 and the control.

Additional key words

gas exchange growth characteristics leaf water status pigments yield characteristics 



carbon dioxide




fruit fresh mass


fresh mass


ferric reducing antioxidant potential


stomatal conductance


leaf dry mass


open top chambers


photosynthetic rate


root dry mass


stem dry mass


specific leaf mass


total dry mass


total soluble solids


water-use efficiency


osmotic potential


water potential


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  1. Agarwal, S., Rao, V.A.: Tomato lycopene and its role in human health and chronic diseases. — Can. Med. Assoc. J. 163: 739–744, 2000.Google Scholar
  2. Ainsworth, E.A., Long, S.P.: What have we learned from 15 years of free-air CO2 enrichment (FACE)? A meta-analysis review of the response of photosynthesis, canopy properties and plant production to rising CO2. — New Phytol. 165: 351–371, 2005.PubMedCrossRefGoogle Scholar
  3. AOAC: Titratable acidity of fruit products. — In: Official Methods of Analysis (17th edn.). 942.15. AOAC International, Gaithersburg 2000.Google Scholar
  4. AOAC: Ascorbic acid. — In: Official Methods of Analysis, 967.21, 45.1.14. AOAC International, Gaithersburg 2006.Google Scholar
  5. Arena, C., Vitale L., Virzo De Santo, A.: Influence of irradiance on photosynthesis and PSII photochemical efficiency in maize during short-term exposure at high CO2 concentration. — Photosynthetica 49: 267–274, 2011.CrossRefGoogle Scholar
  6. Barbale, D.: The influence of the carbon dioxide on the yield and quality of cucumber and tomato in the covered areas. — Augsne un Raza (Riga). 16: 66–73, 1970.Google Scholar
  7. Behboudian, M.H., Lai, R.: Carbon dioxide enrichment in ‘Virosa’ tomato plant: Responses to enrichment duration and to temperature. — Hort. Sci. 29: 1456–1459, 1994.Google Scholar
  8. Benzie, I.F., Strain, J.J.: The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. — Anal. Biochem. 239: 70–76, 1996.PubMedCrossRefGoogle Scholar
  9. Bunce, J.A.: Leaf transpiration efficiency in four corn cultivars grown at elevated carbon dioxide. — Crop Sci. 52: 2714–2717, 2012.CrossRefGoogle Scholar
  10. Carter, T.R., Jones, R.N., Lu, X. et al.: New assessment methods and the characterization of future conditions. — In: Parry, M.L., Canziani, O.F., Palutikof, J.P. et al. (ed.): Contribution of Working Group II to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. Pp. 133–171. Cambridge University Press, Cambridge 2007.Google Scholar
  11. Chowdhury, R.S., Karim, M.A., Haque, M.M. et al.: Effects of enhanced level of CO2 on photosynthesis, nitrogen content and productivity of mungbean (Vigna radiata L. WILCZEK). — South Pacific Studies 25: 97–103, 2005.Google Scholar
  12. Chun, O.K., Kim, D.O., Moon, H.Y. et al.: Contribution of individual polyphenolics to total antioxidant capacity of plums. — J. Agr. Food Chem. 51: 7240–7245, 2003.CrossRefGoogle Scholar
  13. Clark, H., Newton, P.C.D., Barker, D.J.: Physiological and morphological responses to elevated CO2 and a soil moisture deficit of temperate pasture species growing in an established plant community. — J. Exp. Bot. 50: 233–242, 1999.CrossRefGoogle Scholar
  14. Conroy, J.P., Milham, P.J., Mazur, M., Barlow, E.W.: Growth, dry weight partitioning and wood properties of Pinus radiata D. Don after 2 years of CO2 enrichment. — Plant Cell Environ. 13: 329–337, 1990.CrossRefGoogle Scholar
  15. Estiarte, M., Filella, I., Serra, J., Penuelas, J.: Effects of nutrient and water stress on leaf phenolic content of peppers and susceptibility to generalist herbivore Helicoverpa armigera (Hubner). — Oecologia 99: 387–391, 1994.CrossRefGoogle Scholar
  16. Fierro, A., Tremblay, N., Gosselin, A.: Supplemental carbon dioxide and light improved tomato and pepper seedling growth and yield. — Hort. Sci. 29: 152–154, 1994.Google Scholar
  17. Foyer, C.H., Descourvieres, P., Kunert, K.J.: Protection against oxygen radicals: An important defense mechanism studied in transgenic plants. — Plant Cell Environ. 17: 507–523. 1994.CrossRefGoogle Scholar
  18. Haque, M.S., Karim, M.A., Haque, M.M. et al.: Effect of elevated CO2 concentration on growth, chlorophyll content and yield of mungbean (Vigna radiata L. Wilczek) genotypes. — Jap. J. Trop. Agr. 49: 189–196, 2005.Google Scholar
  19. Helyes, L., Lugasi, A., Peli, E., Pek, Z.: Effect of elevated CO2 on lycopene content of tomato (Lycopersicon lycopersicum L. Karsten) fruits. — Acta Aliment. 40: 80–86, 2011.CrossRefGoogle Scholar
  20. Hocking, P.J., Meyer, C.P.: Effect of CO2 enrichment and nitrogen stress on growth, and partitioning of dry matter and nitrogen in wheat and maize. — Aust. J. Plant Physiol. 18: 339–356, 1991.CrossRefGoogle Scholar
  21. Houpis, J.L.J., Surano, K.A., Cowles, S., Shinn, J.H.: Chlorophyll and carotenoid concentrations in two varieties of Pinus ponderosa seedlings subjected to long-term elevated carbon dioxide. — Tree Physiol. 4: 187–193, 1988.PubMedCrossRefGoogle Scholar
  22. Idso, S.B., Kimball, B.A., Shaw, P.E. et al.: The effect of elevated atmospheric CO2 on the vitamin C concentration of (sour) orange juice. — Agr. Ecosyst. Environ. 90: 1–7, 2002.CrossRefGoogle Scholar
  23. IPCC: Summary for policymakers. — In: Solomon, S., Qin, D., Manning, M. et al. (ed.): Climate Change 2007: The physical Science Basis. Contribution of Working Group I to the Fourth Assessment Report of the Inter-governmental Panel on Climate Change. Cambridge University press, Cambridge, New York 2007.Google Scholar
  24. Islam, S., Khan, S., Garner, J.O.: Elevated atmospheric CO2 concentration enhances carbohydrate metabolism in developing Lycopersicon esculentum Mill. cultivars. — Int. J. Agr. Biol. 8:157-161, 2006.Google Scholar
  25. Islam, M.S., Matsui, T., Yoshida, Y.: Effect of carbon dioxide enrichment on physico-chemical and enzymatic changes in tomato fruits at various stages of maturity. — Sci. Hort. 65: 137–149, 1996.CrossRefGoogle Scholar
  26. Kadam, G.B., Singh, K.P., Pal, M.: Effect of elevated carbon dioxide levels on morphological and physiological parameters in gladiolus. — Indian J. Hort. 69: 379–384, 2012.Google Scholar
  27. Kaur, C., Walia, S., Nagal, S. et al.: Functional quality and antioxidant composition of selected tomato (Solanum lycopersicon L.) cultivars grown in Northern India. — Food Sci. Technol. 50: 139–145, 2013.Google Scholar
  28. Kimball, B.A., Mitchell, S.T.: Effects of CO2 enrichment, ventilation, and nutrient concentration on the flavour and vitamin C content of tomato fruit. — Hort. Sci. 16: 665–666, 1981.Google Scholar
  29. Kimball, B.A., Idso, S.B.: Increasing atmospheric CO2: effects on crop yield, water use, and climate. — Agr. Water Manage. 7: 55–72, 1983.CrossRefGoogle Scholar
  30. Kimball, B.A., Kobayashi, K., Bindi, M.: Response of agricultural crops to free air CO2 enhancement. — Adv. Agron. 77: 293–368, 2002.CrossRefGoogle Scholar
  31. Leakey, A.D.B., Xu, F., Gillespie, K.M. et al.: The genomic basis for stimulated respiratory carbon loss to the atmosphere by plants growing under elevated CO2. — P. Natl. Acad. Sci. USA 106: 3597–3602, 2009.CrossRefGoogle Scholar
  32. Leonardi, C., Ambrosino, P., Esposito, F., Fogliano, V.: Antioxidant activity and carotenoid and tomatine contents in different typologies of fresh consumption tomatoes. — J. Agr. Food Chem. 48: 4723–4727, 2000.CrossRefGoogle Scholar
  33. Li, F.S., Kang, S.Z., Zhang, J.H.: Interactive effects of elevated CO2, nitrogen and drought on leaf area, stomatal conductance, and evapotranspiration of wheat. — Agr. Water Manage. 67: 221–233, 2004.CrossRefGoogle Scholar
  34. Li, Y., Zhang, Y., Zhang, X. et al.: Effects of elevated CO2 and temperature on photosynthesis and leaf traits of an understory dwarf bamboo in subalpine forest zone, China. — Physiol. Plantarum 148: 261–272, 2013.CrossRefGoogle Scholar
  35. Lichtenthaler, H.K.: Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. — Method. Enzymol. 148: 350–382, 1987.CrossRefGoogle Scholar
  36. Long, S.P., Ainsworth, E.A., Rogers, A., Ort, D.R.: Rising atmospheric carbon dioxide: Plants FACE the future. — Ann. Rev. Plant Biol. 55: 591–628, 2004.CrossRefGoogle Scholar
  37. Mackinney, G.: Absorption of light by chlorophyll solution. — J. Biol. Chem. 140: 315–322, 1941.Google Scholar
  38. Madsen, E.: Effect of CO2 environment on growth, development, fruit production and fruit quality of tomato from a physiological viewpoint. — In: Chouard, P., de Bilderling, N. (ed.): Phytotronics in Agricultural and Horticultural Research. Pp. 318–330. Bordas, Paris 1975.Google Scholar
  39. Madsen, E.: The influence of CO2-concentration on the content of ascorbic acid in tomato leaves. — Ugeskr. Agron. 116: 592–594, 1971.Google Scholar
  40. Mbikayi, N.T., Hileman, D.R., Bhattacharya, N.C. et al.: Effects of CO2 enrichment on the physiology and biomass production in cowpeas (Vigna ungiculata L.) grown in open top chambers. — In: Proceedings of the International Congress of Plant Physiology 1. Pp. 640–645. India Society for Plant Physiology and Biochemistry, New Delhi 1988.Google Scholar
  41. Moretti, C.L., Mattos, L.M., Calbo, A.G., Sargent, S.A.: Climate changes and potential impacts on postharvest quality of fruit and vegetable crops - a review. — Food Res. Int. 43: 1824–1832, 2010.CrossRefGoogle Scholar
  42. Nilsen, S., Hovland, K., Dons, C., Sletten, S.P.: Effect of CO2 enrichment on photosynthesis, growth and yield of tomato. — Sci. Hort. 20: 1–14, 1983.CrossRefGoogle Scholar
  43. Peet, M.M., Willits, D.H., Tripp, K.E. et al.: CO2 enrichment responses of chrysanthemum, cucumber and tomato: photosynthesis, growth, nutrient concentrations and yield. Impact of global climatic changes on photosynthesis and plant productivity. — In: Proceedings of the Indo-US workshop held on 8 - 12 January at New Delhi, India: Pp. 193–212, New Delhi 1991.Google Scholar
  44. Petridis, A., Therios, I., Samouris, G. et al.: Effect of water deficit on leaf phenolic composition, gas exchange, oxidative damage and antioxidant activity of four Greek olive (Olea europaea L.) cultivars. — Plant Physiol. Biochem. 60: 1–11, 2012.PubMedCrossRefGoogle Scholar
  45. Radford, P.J.: Growth analysis formulae: Their use and abuse. — Crop Sci. 8: 171–175, 1967.CrossRefGoogle Scholar
  46. Rao, M.V., Hale, B.A., Ormrod, D.P.: Amelioration of ozoneinduced oxidative damage in wheat plants grown under high carbon dioxide. Role of antioxidant enzymes. — Plant Physiol. 109: 421–432, 1995.PubMedCentralPubMedGoogle Scholar
  47. Reddy, A.R., Rasineni, G.K., Raghavendra, A.S.: The impact of global elevated CO2 concentration on photosynthesis and plant productivity. — Curr. Sci. 99: 46–57, 2010.Google Scholar
  48. Reinert, R.A., Eason, G., Barton, J.: Growth and fruiting of tomato as influenced by elevated carbon dioxide and ozone. — New Phytol. 137: 411–420, 1997.CrossRefGoogle Scholar
  49. Rogers, H.H., Runion, G.B., Krupa, S.V.: Plant responses to atmospheric CO2 enrichment with emphasis on roots and rhizosphere. — Environ. Pollut. 83: 155–189, 1994.PubMedCrossRefGoogle Scholar
  50. Shivashankara, K.S., Acharya, S.N.: Bioavailability of dietary polyphenols and the cardiovascular diseases. — Open Nutraceuticals J. 3: 227–241, 2010.CrossRefGoogle Scholar
  51. Shivashankara, K.S., Rao, N.K.S., Geetha, G.A.: Impact of climate change on fruit and vegetable quality. — In: Singh, H.P., Rao, N.K.S, Shivashankara, K.S. (ed.): Climate-resilient Horticulture: Adaptation and Mitigation Strategies. Pp. 237–244. Springer, New Delhi, Heidelberg, New York, Dordrecht, London 2013.Google Scholar
  52. Shoaf, T.W., Lium, B.W.: Improved extraction of chlorophyll a and b from algae using dimethyl sulfoxide. — Limnol. Oceanogr. 21: 926–928, 1976.CrossRefGoogle Scholar
  53. Shwartz, M.: High carbon dioxide levels can retard plant growth, study reveals. — Stanford Report, December 11: 1–5, 2002.Google Scholar
  54. Sicher, R.C., Bunce, J.A.: Relationship of photosynthetic acclimation to changes of Rubisco activity in field-grown winter wheat and barley during growth in elevated carbon dioxide. — Photosynth. Res. 52: 27–38, 1997.CrossRefGoogle Scholar
  55. Singleton, V.J., Rossi, J.A.: Colorimetry of total phenolics with phospho-molybdicphosphotungstic acid reagents. — Am. J. Enol. Viticult. 16: 144–158, 1965.Google Scholar
  56. Sionit, N., Strain, B.R., Hellmers, H., Kramer, P.J.: Effects of atmospheric CO2 concentrations and water stress on water relations of wheat. — Bot. Gaz. 142: 191–196, 1981.CrossRefGoogle Scholar
  57. Stewart, A.J., Bozonnet, S., Mullen, W. et al.: Occurrence of flavonols in tomatoes and tomato-based products. — J. Agr. Food Chem. 48: 2663–2669, 2000.CrossRefGoogle Scholar
  58. Tajiri, T.: Improvement of bean sprouts production by intermittent treatment with carbon dioxide. — Nippon Shokuhin Kogyo Gakkaishi 32: 159–169, 1985.CrossRefGoogle Scholar
  59. Thomas, J.F., Harvey, C.N.: Leaf anatomy of four species grown under continuous CO2 enrichment. — Bot. Gaz. 14: 303–309, 1983.CrossRefGoogle Scholar
  60. Tyree, M.T., Alexander, J.D.: Plant water relations and the effects of elevated CO2: a review and suggestions for future research. — Vegetatio 104/105: 47–62, 1993.CrossRefGoogle Scholar
  61. von Caemmerer, S., Farquhar, G.D.: Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. — Planta 153: 376–387, 1981.CrossRefGoogle Scholar
  62. Wang, S.Y., Bunce, J.A., Maas, J.L.: Elevated carbon dioxide increases contents of antioxidant compounds in field grown strawberries. — J. Agr. Food. Chem. 51: 4315–4320, 2003.CrossRefGoogle Scholar
  63. Wullschleger, S.D., Norby, R.J., Hendrix, D.L.: Carbon exchange rates, chlorophyll content, and carbohydrate status of two forest tree species exposed to carbon dioxide enrichment — Tree Physiol. 10: 21–31, 1992.PubMedCrossRefGoogle Scholar
  64. Wullschleger, S.D., Tschaplinski, T.J., Norby, R.J.: Plant water relations at elevated CO2 - implications for water-limited environments. — Plant Cell Environ. 25: 319–331, 2002.PubMedCrossRefGoogle Scholar
  65. Recent monthly average Mauna Loa CO2. — August 2013.
  66. Yelle, S., Beeson Jr., R.C., Trudel, M.J., Gosselin, A.: Duration of CO2 enrichment influences growth, yield, and gas exchange of two tomato species. — J. Am. Soc. Hort. Sci. 115: 52–57, 1990.Google Scholar
  67. Zhao, H., Xu, X., Zhang, Y., Korpelainen, H., Li, C.: Nitrogen deposition limits photosynthetic response to elevated CO2 differentially in a dioecious species. — Oecologia 165: 41–45, 2011.PubMedCrossRefGoogle Scholar

Copyright information

© The Institute of Experimental Botany 2014

Authors and Affiliations

  • H. Mamatha
    • 1
  • N. K. Srinivasa Rao
    • 1
    Email author
  • R. H. Laxman
    • 1
  • K. S. Shivashankara
    • 1
  • R. M. Bhatt
    • 1
  • K. C. Pavithra
    • 1
  1. 1.Division of Plant Physiology and BiochemistryIndian Institute of Horticultural ResearchBangaloreIndia

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