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Effect of high temperature on dehydration-induced alterations in photosynthetic characteristics of the resurrection plant Haberlea rhodopensis

  • Original Papers
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Photosynthetica

Abstract

The effect of high temperature (HT) and dehydration on the activity of photosynthetic apparatus and its ability to restore membrane properties, oxygen evolution, and energy distribution upon rehydration were investigated in a resurrection plant, Haberlea rhodopensis. Plants growing under low irradiance in their natural habitat were desiccated to air-dry state at a similar light intensity [about 30 μol(photon) m−2 s−1] under optimal day/night (23/20°C) or high (38/30°C) temperature. Our results showed that HT alone reduced the photosynthetic activity and desiccation of plants at 38°C and it had more detrimental effect compared with desiccation at 23°C. The study on isolated thylakoids demonstrated increased distribution of excitation energy to PSI as a result of the HT treatment, which was enhanced upon the desiccation. It could be related to partial destacking of thylakoid membranes, which was confirmed by electron microscopy data. In addition, the surface charge density of thylakoid membranes isolated from plants desiccated at 38°C was higher in comparison with those at 23°C, which was in agreement with the decreased membrane stacking. Dehydration led to a decrease of amplitudes of oxygen yields and to a loss of the oscillation pattern. Following rehydration, the recovery of CO2 assimilation and fluorescence properties were better when desiccation was performed at optimal temperature compared to high temperature. Rehydration resulted in partial recovery of the amplitudes of flash oxygen yields as well as of population of S0 state in plants desiccated at 23°C. However, it was not observed in plants dehydrated at 38°C.

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Abbreviations

BSA:

bovine serum albumin

Chl a :

chlorophyll a

Chl b :

chlorophyll b

E :

transpiration

EPM:

electrophoretic mobility

HEPES:

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

HT:

high temperature

g s :

stomatal conductance

LHCI:

light-harvesting complex of photosystem I

LHCII:

light-harvesting complex of photosystem II

MES:

2-[N-morpholino] ethanesulfonic acid

OE:

oxygen-evolving

P N :

net photosynthetic rate

PS:

photosystem

RWC:

relative water content

σ:

surface charge density

ζ:

zeta potential.

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Authors and Affiliations

  1. Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev str. Bl. 21, 1113, Sofia, Bulgaria

    M. Velitchkova

  2. Faculty of Biology, Sofia University “St. K. Ohridski”, 8 Dragan Tsankov Blvd., 1164, Sofia, Bulgaria

    V. Doltchinkova

  3. Faculty of Medicine, Sofia University “St. K. Ohridski”, 1 Kozyak str., 1407, Sofia, Bulgaria

    D. Lazarova

  4. Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev str. Bl. 21, 1113, Sofia, Bulgaria

    G. Mihailova, S. Doncheva & K. Georgieva

Authors
  1. M. Velitchkova
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  2. V. Doltchinkova
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  3. D. Lazarova
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  4. G. Mihailova
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  5. S. Doncheva
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  6. K. Georgieva
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Corresponding author

Correspondence to K. Georgieva.

Additional information

Acknowledgement: This work is supported by National Science Fund of Bulgaria under Research project дO02-208/2008.

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Velitchkova, M., Doltchinkova, V., Lazarova, D. et al. Effect of high temperature on dehydration-induced alterations in photosynthetic characteristics of the resurrection plant Haberlea rhodopensis . Photosynthetica 51, 630–640 (2013). https://doi.org/10.1007/s11099-013-0063-9

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  • DOI: https://doi.org/10.1007/s11099-013-0063-9

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