, Volume 51, Issue 4, pp 574–582 | Cite as

Effects of drought stress on the evergreen Quercus ilex L., the deciduous Q. robur L. and their hybrid Q. × turneri Willd.

  • S. KollerEmail author
  • V. Holland
  • W. Brüggemann
Original Papers


Five-year-old trees of deciduous Quercus robur L., evergreen Q. ilex L., and their semideciduous hybrid, Q. × turneri Willd. (var. pseudoturneri), growing in pots, were subjected to drought stress by withholding water for 18–22 days, until leaf water potentials decreased below −2 MPa. Gas-exchange rates, oxygen evolution, and modulated chlorophyll (Chl) fluorescence measurements revealed that by strong stomata closure and declining photosynthetic capacity down to approximately 50%, all three taxa responded with strongly reduced photosynthesis rates. In Q. robur, photochemical quenching of the drought-stressed plants was much lower than in nonstressed controls. Dissection of the occurring events in the photosynthetic electron transport chain by fast Chl fluorescence induction analysis with the JIP-test were discussed.

Additional key words

chlorophyll fluorescence gas exchange oak OJIP-test Quercus 





intercellular CO2 mole fraction


drought stress


electron transport


maximal variable fluorescence


maximum quantum yield of primary photochemistry


stomatal conductance of water vapour


leaf water content


nonphotochemical quenching




photosynthetically active radiation


photosynthetic capacity (capacity of the photosynthetic system, if photorespiration and stomatal effects are avoided due to high CO2 concentration)


net photosynthetic rate


performance index on absorption basis






photoinhibitory quenching


photochemical quenching


reaction centre


relative variable fluorescence at I-step (30 μs)


relative variable fluorescence at I-step (30 μs)


relative variable fluorescence at K-step (300 μs)


IP-phase (1-VI)


quantum yield of energy dissipation


quantum yield of electron transport


maximum quantum yield of primary photochemistry


predawn leaf water potential


probability that a trapped exciton moves an electron into the electron transport chain beyond QA


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  1. Björkman, O., Demmig, B.: Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77K among vascular plants of diverse origins. — Planta 170: 489–504, 1987.CrossRefPubMedGoogle Scholar
  2. Brüggemann, W.: Low-temperature limitations of photosynthesis in three tropical Vigna species: A chlorophyll fluorescence study. — Photosynth. Res. 34: 301–310, 1992.CrossRefPubMedGoogle Scholar
  3. Bussotti, F.: Assessment of stress conditions in Quercus ilex L. leaves by O-J-I-P chlorophyll a fluorescence analysis. — Plant Biosyst. 138: 101–109, 2004.CrossRefGoogle Scholar
  4. Cavender-Bares, J., Holbrook, N.M.: Hydraulic properties and freezing-induced cavitation in sympatric evergreen and deciduous oaks with contrasting habitats. — Plant Cell Environ. 24: 1243–1256, 2001.CrossRefGoogle Scholar
  5. Demmig-Adams, B., Adams, W.W., III: Xanthophyll cycle and light stress in nature: uniform response to excess direct sunlight among higher plant species. — Planta 198: 460–470, 1996.CrossRefGoogle Scholar
  6. Dinç E., Tóth S.Z., Bottka S. et al.: The chl a fluorescence intensity is remarkably insensitive to changes in the chlorophyll content of the leaf as long as the chl a/b ratio remains unaffected. — Biochim. Biophys. Acta 1817: 770–779, 2012.CrossRefPubMedGoogle Scholar
  7. Epron, D., Dreyer, E.: Long-term effects of drought on photosynthesis of adult oak trees (Quercus petraea (Matt.) Liebl. and Quercus robur L.) in a natural stand. — New Phytol. 125: 381–389, 1993.CrossRefGoogle Scholar
  8. Filella, I., Llusia, J., Piñol, J., Peñuelas, J.: Leaf gas exchange and fluorescence of Phillyrea latifolia, Pistacia lentiscus and Quercus ilex saplings in severe drought and high temperature conditions. — Environ. Exp. Bot. 39: 213–220, 1998.CrossRefGoogle Scholar
  9. Gallé, A., Haldimann, P., Feller, U.: Photosynthetic performance and water relations in young pubescent oak (Quercus pubescens) trees during drought stress and recovery. — New Phytol. 174: 799–810, 2007.CrossRefPubMedGoogle Scholar
  10. Gilmore, A.M.: Mechanistic aspects of xanthophyll cycledependent photoprotection in higher plant chloroplasts and leaves. — Physiol. Plant. 99: 197–209, 1997.CrossRefGoogle Scholar
  11. Gratani, L., Pesoli, P., Crecente, M.F., Aichner, K., Larcher, W.: Photosynthesis as a temperature indicator in Quercus ilex L. — Global Planet. Change 24: 153–163, 2000.CrossRefGoogle Scholar
  12. Grassi, G., Magnani, F.: Stomatal, mesophyll conductance and biochemical limitations to photosynthesis as affected by drought and leaf ontogeny in ash and oak trees. — Plant Cell Environ. 28: 834–849, 2005.CrossRefGoogle Scholar
  13. Haldimann, P., Gallé, A., Feller, U.: Impact of an exceptionally hot dry summer on photosynthesis traits in oak (Quercus pubescens) leaves. — Tree Physiol. 28: 785–795, 2008.CrossRefPubMedGoogle Scholar
  14. Huber, B.: [Further quantitative analysis about the hydraulic water system in plants]. — Jahrb. Wiss. Bot. 67: 877–959, 1928. [In German].Google Scholar
  15. Jedmowski, C., Ashoub, A., Brüggemann, W.: Reactions of Egyptian landraces of Hordeum vulgare and Sorghum bicolor to drought stress, evaluated by the OJIP fluorescence transient analysis. — Acta Physiol. Plant. 35: 345–354, 2013.CrossRefGoogle Scholar
  16. Koller S., Jedmowski, C., Kamm, K., Brüggemann, W.: The South Hesse Oak Project (SHOP): Species- and site-specific efficiency of the photosynthetic apparatus of Mediterranean and Central European Oaks — Plant Biosystems, doi 10.1080/11263504.2012.762947, 2013.Google Scholar
  17. Krause, G.H.: Photoinhibiotn of photosynthesis. An evaluation of damaging and protective mechanisms. — Physiol. Plant. 74: 566–574, 1988.CrossRefGoogle Scholar
  18. Krause G.H., Weis E.: Chlorophyll fluorescence and photosynthesis: The basics. — Annu. Rev. Plant. Physiol. Plant. Mol. Biol 42: 313–349, 1991.CrossRefGoogle Scholar
  19. Manes, F., Vitale, M., Donato, E., Giannini, M., Puppi, G.: Different ability of three Mediterranean oak species to tolerate progressive water stress. — Photosynthetica 44: 387–393, 2006.CrossRefGoogle Scholar
  20. Mediavilla, S., Escudero, A.: Stomatal responses to drought of mature trees and seedlings of two co-occuring Mediterranean oaks. — Forest Ecol. Manag. 187: 281–294, 2004.CrossRefGoogle Scholar
  21. Mediavilla, S., Santiago, H., Escudero, A.: Stomatal and mesophyll limitations to photosynthesis in one evergreen and one deciduous Mediterranean oak species. — Photosynthetica 40: 553–559, 2002.CrossRefGoogle Scholar
  22. Méthy, M., Damesin, C., Rambal, S.: Drought and photosystem II activity in two Mediterranean oaks. — Ann. Forest Sci. 53: 255–262, 1996.CrossRefGoogle Scholar
  23. Nardini, A., Lo Gullo, M.A., Salleo, S.: Competitive strategies for water availability in two Mediterranean Quercus species. — Plant Cell Environ. 22: 109–116, 1999.CrossRefGoogle Scholar
  24. Ogaya, R., Peñuelas, J. Comparative field study of Quercus ilex and Phillyrea latifolia: photosynthetic response to experimental drought conditions. — Environ. Exp. Bot. 50: 137–148, 2003.CrossRefGoogle Scholar
  25. Oukarroum, A., El Madidi, S., Schansker, G., Strasser, R.J.: Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering. — Environ. Exp. Bot. 60: 438–446, 2007.CrossRefGoogle Scholar
  26. Oukarroum, A., Schansker, G., Strasser, R.J.: Drought stress effects on photosystem I content and photosystem II thermotolerance analyzed using Chl a fluorescence kinetics in barley varieties differing in their drought tolerance. — Physiol. Plant. 137: 188–189, 2009.CrossRefPubMedGoogle Scholar
  27. Oxborough, K., Baker, NR.: Resolving chlorophyll a fluorescence images of photosynthetic efficiency into photochemical and non-photochemical components — calculation of qP and Fv′/Fm′; without measuring F0′. — Photosynth. Res. 54: 135–142, 1997.CrossRefGoogle Scholar
  28. Peña-Rojas, K., Aranda, X., Fleck, I.: Stomatal limitation to CO2 assimilation and down-regulation of photosynthesis in Quercus ilex resprouts in response to slowly imposed drought. — Tree Physiol. 24: 813–822, 2004.CrossRefPubMedGoogle Scholar
  29. Percival, G.C.: The use of chlorophyll fluorescence to identify chemical and environmental stress in leaf tissue of three oak (Quercus) species. — J. Arboricult. 31: 215–227, 2005.Google Scholar
  30. Quick, W.P., Stitt, M.: An examination of factors contributing to non-photochemical quenching of chlorophyll fluorescence in barley leaves. — Biochim. Biophys. Acta 977: 287–296, 1989.CrossRefGoogle Scholar
  31. Scarascia-Muignozza, G., De Angelis, P., Matteucci, G., Valentini, R.: Long-term exposure to elevated [CO2] in a natural Quercus ilex L. community: net photosynthesis and photochemical efficiency of PSII at different levels of water stress. — Plant Cell Environ. 19: 643–654, 1996.CrossRefGoogle Scholar
  32. Schär, C., Vidale, P.L., Lüthi, D., Frei, C., Häberli, C., Liniger, M.A., Appenzeller, C.: The role of increasing temperature variability in European summer heatwaves. — Nature 427: 332–336, 2004.CrossRefPubMedGoogle Scholar
  33. Schmiedinger, A., Bachmann, M., Kölling, C., Schirmer, R.: [How to select tree species for trials against the background of climate change.] — Forstarchiv. 80: 15–22, 2009. [In German]Google Scholar
  34. Schreiber, U., Schliwa, U., Bilger, W.: Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. — Photosynth. Res. 10: 51–62, 1986.CrossRefPubMedGoogle Scholar
  35. Strasser, B.J.: Donor side capacity of Photosystem II probed by chlorophyll a fluorescence transients. — Photosynth. Res. 52: 147–155, 1997.CrossRefGoogle Scholar
  36. Strasser B.J., Strasser R.J.: Measuring fast fluorescence transients to address environmental questions: The JIP-test. — In: Mathis P. (ed.): Photosynthesis: From Light to Biosphere. Vol V: 977–980. Kluwer Academic Publ., Dordrecht 1995.Google Scholar
  37. Strasser, R.J., Srivastava, A., Tsimilli-Michael, M.: The fluorescence transient as a tool to characterize and screen photosynthetic samples. — In: Yunus, M., Pathre, U., Mohanty, P. (ed.): Probing Photosynthesis: Mechanisms, Regulation and Adaptation. Pp. 445–483. Taylor & Francis, London 2000.Google Scholar
  38. Strasser, R.J., Tsimilli-Michael, M., Srivastava, A.: Analysis of the fluorescence transient. — In: Papageorgiou, G.C., Govindjee (ed.): Chlorophyll Fluorescence: A Signature of Photosynthesis. Advances in Photosynthesis and Respiration Vol. 19: 321–362, Kluwer Acad. Publ., Dordrecht 2004.CrossRefGoogle Scholar
  39. Strasser, R.J., Tsimilli-Michael, M., Qiang, S., Goltsev, V.: Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis. — Biochim. Biophys. Acta 1797: 1313–1326, 2010.CrossRefPubMedGoogle Scholar
  40. Tsimilli-Michael, M., Strasser, R.J.: In vivo assessment of stress impact on plant’s vitality: Applications in detecting and evaluating the beneficial role of mycorrhization on host plants. — In: Varma, A. (ed.): Mycorrhiza. Pp. 679–703. Springer, Berlin — Heidelberg 2008.CrossRefGoogle Scholar
  41. Van Heerden, P.D.R., Swanepoel J.W., Krüger, G.H.J.: Modulation of photosynthesis by drought in two desert scrub species exhibiting C3-mode of CO2 assimilation. — Environ. Exp. Bot. 61: 124–136, 2007.CrossRefGoogle Scholar
  42. von Caemmerer, S., Farquhar, G.D.: Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. — Planta 153: 376–387, 1981.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Biodiversity and Climate Research Center Frankfurt (BiK-F)FrankfurtGermany
  2. 2.Department of Ecology, Evolution and DiversityGoethe University FrankfurtFrankfurtGermany

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