Responses of cotton and wheat photosynthesis and growth to cyclic variation in carbon dioxide concentration

Abstract

The carbon dioxide concentration in free air carbon dioxide enrichment (FACE) systems typically has rapid fluctuations. In our FACE system, power spectral analysis of CO2 concentration measured every second with an open path analyzer indicated peaks in variation with a period of about one minute. I used open-top chambers to expose cotton and wheat plants to either a constant elevated CO2 concentration of 180 μmol mol−1 above that of outside ambient air, or to the same mean CO2 concentration, but with the CO2 enrichment cycling between about 30 and 330 μmol mol−1 above the concentration of outside ambient air, with a period of one minute. Three short-term replicate plantings of cotton were grown in Beltsville, Maryland with these CO2 concentration treatments imposed for 27-day periods over two summers, and one winter wheat crop was grown from sowing to maturity. In cotton, leaf gas-exchange measurements of the continuously elevated treatment and the fluctuating treatment indicated that the fluctuating CO2 concentration treatment consistently resulted in substantial down-regulation of net photosynthetic rate (P N) and stomatal conductance (g s). Total shoot biomass of the vegetative cotton plants in the fluctuating CO2 concentration treatment averaged 30% less than in the constantly elevated CO2 concentration treatment at 27 days after planting. In winter wheat, leaf gas-exchange measurements also indicated that down-regulation of P N and g s occurred in flag leaves in the fluctuating CO2 concentration treatment, but the effect was not as consistent in other leaves, nor as severe as found in cotton. However, wheat grain yields were 12% less in the fluctuating CO2 concentration treatment compared with the constant elevated CO2 concentration treatment. Comparison with wheat yields in chambers without CO2 addition indicated a nonsignificant increase of 5% for the fluctuating elevated CO2 concentration treatment, and a significant increase of 19% for the constant elevated treatment. The results suggest that treatments with fluctuating elevated CO2 concentrations could underestimate plant growth at projected future atmospheric CO2 concentrations.

This is a preview of subscription content, access via your institution.

Abbreviations

C i :

CO2 concentration in the substomatal (intercellular) airspace

FACE:

free air carbon dioxide enrichment

g s :

stomatal conductance

P N :

net photosynthetic rate

References

  1. Bounoua, L., Collatz, G.J., Sellers, P.J. et al.: Interaction between vegetation and climate: radiative and physiological effects of doubled atmospheric CO2. — J. Climate 12: 309–324, 1999.

    Article  Google Scholar 

  2. Bunce, J.A.: Performance characteristics of an area distributed free air carbon dioxide enrichment (FACE) system. — Agr. Forest Meteorol. 151: 1152–1157, 2011.

    Article  Google Scholar 

  3. Cardon, Z.G., Berry, J.A., Woodrow, I.E.: Evidence of the extent and direction of average stomatal response in Zea mays L. and Phaseolus vulgaris L. on the frequency of fluctuations in environmental stimuli. — Plant Physiol. 105: 1007–1013, 1994.

    PubMed  CAS  Google Scholar 

  4. Hendrey, G.R., Long, S.P., McKee, I.F., Baker, N.R.: Can photosynthesis respond to short-term fluctuations in atmospheric carbon dioxide? — Photosynth. Res. 51: 179–184, 1997.

    Article  CAS  Google Scholar 

  5. Hendrey, G.R., Ellsworth, D.S., Lewin, K.F., Nagy, J.: A freeair enrichment systems for exposing tall forest vegetation to elevated atmospheric CO2. — Global Change Biol. 5: 293–309, 1999.

    Article  Google Scholar 

  6. Holtum, J.A.M., Winter, K.: Photosynthetic CO2 uptake in seedlings of two tropical tree species exposed to oscillating elevated concentrations of CO2. — Planta 218: 152–158, 2003.

    PubMed  Article  CAS  Google Scholar 

  7. Kimball, B.A., Pinter, P.J.,Jr., Wall, G.W. et al.: Comparisons of responses of vegetation to elevated carbon dioxide in freeair and open-top chamber facilities. — In: Allen, L.H.,Jr., Kirkham, M.B., Olszyk, D.M., Whitman, C.E. (ed.): Advances in Carbon Dioxide Research. Pp.113–130. Amer. Soc. Agron., Crop Sci. Soc. Amer., and Soil Sci. Soc. Amer., Madison 1997.

    Google Scholar 

  8. Okada, M., Lieffering, H., Nakamura, H., Yoshimoto, M., Kim, H.Y., Kobayashi, K.: Free-air CO2 enrichment (FACE) using pure CO2 injection: system description. — New Phytol. 150: 251–260, 2001.

    Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to J. A. Bunce.

Additional information

Acknowledgements: Dr. Bruce Kimball suggested the use of open-top chambers and a solenoid value system to achieve the cyclic elevated carbon dioxide treatment.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bunce, J.A. Responses of cotton and wheat photosynthesis and growth to cyclic variation in carbon dioxide concentration. Photosynthetica 50, 395–400 (2012). https://doi.org/10.1007/s11099-012-0041-7

Download citation

Additional key words

  • acclimation
  • down-regulation
  • stomatal conductance