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Characterization of different isoforms of the light-harvesting chlorophyll a/b complexes of photosystem II in bamboo

  • Published:
Photosynthetica

Abstract

The major light-harvesting chlorophyll (Chl) a/b complexes of photosystem II (LHCIIb) play important roles in energy balance of thylakoid membrane. They harvest solar energy, transfer the energy to the reaction center under normal light condition and dissipate excess excitation energy under strong light condition. Many bamboo species could grow very fast even under extremely changing light conditions. In order to explain whether LHCIIb in bamboo contributes to this specific characteristic, the spectroscopic features, the capacity of forming homotrimers and structural stabilities of different isoforms (Lhcb1-3) were investigated. The apoproteins of the three isoforms of LHCIIb in bamboo are overexpressed in vitro and successfully refolded with thylakoid pigments. The sequences of Lhcb1 and Lhcb2 are similar and they are capable of forming homotrimer, while Lhcb3 lacks 10 residues in the N terminus and can not form the homotrimeric structure. The pigment stoichiometries, spectroscopic characteristics, thermo- and photostabilities of different reconstituted Lhcbs reveal that Lhcb3 differs strongly from Lhcb1 and Lhcb2. Lhcb3 possesses the lowest Qy transition energy and the highest thermostability. Lhcb2 is the most stable monomer under strong illumination among all the isoforms. These results suggest that in spite of small differences, different Lhcb isoforms in bamboo possess similar characteristics as those in other higher plants.

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Abbreviations

CD:

circular dichroism

Chl:

chlorophyll

DM:

dodecyl β-D-maltoside

HPLC:

high performance liquid chromatography

LDS:

lithium dodecyl sulfate

Lhcb1-3:

three isoforms of light harvesting chlorophyll a/b complexes of PSII

LHCIIb:

light-harvesting chlorophyll a/b complexes of PSII

Lut:

luteins

Neo:

neoxanthin

NPQ:

nonphotochemical quenching

OG:

octyl-β-D-glucopyranoside

PAGE:

polyacrylamide gel electrophoresis

PG:

1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-1-glycerol]

PS:

photosystem

RT:

room temperature

Vio:

violaxanthin

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Authors and Affiliations

  1. International Center for Bamboo and Rattan, State Forestry Administration Key Open Laboratory on Bamboo and Rattan Science and Technology, Beijing, 100102, P.R. China

    Z. H. Jiang, Z. H. Peng & Z. M. Gao

  2. Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, P.R. China

    C. Liu & C. H. Yang

Authors
  1. Z. H. Jiang
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  2. Z. H. Peng
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  3. Z. M. Gao
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  4. C. Liu
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  5. C. H. Yang
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Corresponding authors

Correspondence to C. Liu or C. H. Yang.

Additional information

Acknowledgements: This work was supported by the special funds for scientific research on forestry public causes (No. 200704001), the National Natural Science Foundation of China (No. 30800069, 30972328 and 31070212).

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Jiang, Z.H., Peng, Z.H., Gao, Z.M. et al. Characterization of different isoforms of the light-harvesting chlorophyll a/b complexes of photosystem II in bamboo. Photosynthetica 50, 129–138 (2012). https://doi.org/10.1007/s11099-012-0009-7

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  • DOI: https://doi.org/10.1007/s11099-012-0009-7

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