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The influence of low temperature on photosynthesis and antioxidant enzymes in sensitive banana and tolerant plantain (Musa sp.) cultivars

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Photosynthetica

Abstract

Low temperature (LT) is one of the major factors that limit crop production and reduce yield. To better understand the cold-tolerance mechanism in the plantains, a sensitive cultivar Williams (Musa acuminata AAA cv. Williams) and a tolerant cultivar Cachaco (Musa paradisiaca ABB cv. Dajiao) were used. LT resulted in increased malondialdehyde (MDA) content, elevated contents of hydrogen peroxide (H2O2) and superoxide radical (O ·−2 ), and decreased photochemical efficiency (Fv/Fm) and net photosynthetic rate (P N), but cv. Cachaco showed better LT tolerance than cv. Williams. After LT treatment for 120 h, total scavenging capability (DPPH· scavenging capability) in Williams showed a significant decrease but no significant alternations was found in Cachaco. Ascorbate peroxidase (APX) and peroxidase (POD) displayed a significant increase but superoxide dismutase (SOD) showed no significant alternations and catalase (CAT) showed a significant decrease in Cachaco after 120 h of LT treatment. All the four antioxidant enzymes above showed a significant decrease in Williams after 120 h of LT treatment. Our results suggest that higher activities of APX, POD, SOD, and DPPH· scavenging capability to a certain extent can be used to explain the higher cold tolerance in the plantain, which would provide a theoretical guidance for bananas production and screening cold-resistant variety.

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Abbreviations

APX:

ascorbate peroxidase

ASC:

ascorbate

CAT:

catalase

DAB:

diaminobenzidine

DPPH· :

1,1-diphenyl-2-picrylhydrazyl

Fv/Fm :

maximum photochemical efficiency of photosystem II

g s :

stomatal conductance

H2O2 :

hydrogen peroxide

LT:

low temperature

MDA:

malondialdehyde

NBT:

nitroblue tetrazolium

O ·−2 :

superoxide radical

1O2 :

singlet oxygen

·OH:

hydroxyl radical

P N :

net photosynthetic rate

POD:

peroxidase

SOD:

superoxide dismutase

TCA:

trichloroacetic acid

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Acknowledgments

This research is financially supported by Guangxi Natural Science Foundation Program (No.0991078).

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Authors and Affiliations

  1. College of Life Science, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, South China Normal University, Guangzhou, 510631, China

    Q. Zhang, L. L. Sun, Y. J. Chen & C. L. Peng

  2. Guangxi Key Laboratory of Biotechnology of Crop Genetic Improvement, Nanning, 530007, China

    J. Z. Zhang

  3. Institute of Biotechnology of Guangxi Academy of Agricultural Sciences, Nanning, 530007, China

    J. Z. Zhang

  4. Division of Plant Sciences, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University, Canberra, Australian Capital Territory 0200, Australia

    W. S. Chow

  5. Department of Crop Science, Yunnan Agricultural University, Kunming, 650201, Yunnan, China

    J. W. Chen

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  1. Q. Zhang
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Correspondence to C. L. Peng.

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These authors contributed equally to this work.

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Zhang, Q., Zhang, J.Z., Chow, W.S. et al. The influence of low temperature on photosynthesis and antioxidant enzymes in sensitive banana and tolerant plantain (Musa sp.) cultivars. Photosynthetica 49, 201–208 (2011). https://doi.org/10.1007/s11099-011-0012-4

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  • DOI: https://doi.org/10.1007/s11099-011-0012-4

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