Abstract
To investigate damaging mechanisms of chilling and salt stress to peanut (Arachis hypogaea L.) leaves, LuHua 14 was used in the present work upon exposure to chilling temperature (4°C) accompanied by high irradiance (1,200 μmol m−2 s−1) (CH), salt stress accompanied by high irradiance (1,200 μmol m−2 s−1) (SH), and high-irradiance stress (1,200 μmol m−2 s−1) at room temperature (25°C) (NH), respectively. Additionally, plants under low irradiance (100 μmol m−2 s−1) at room temperature (25°C) were used as control plants (CK). Relative to CK and NH treatments, both the maximal photochemical efficiency of PSII (Fv/Fm) and the absorbance at 820 nm decreased greatly in peanut leaves under CH and SH stress, which indicated that severe photoinhibition occurred in peanut leaves under such conditions. Initial fluorescence (Fo), 1 − qP and nonphotochemical quenching (NPQ) in peanut leaves significantly increased under CH- and SH stress. Additionally, the activity of superoxide dismutase (SOD), one of the key enzymes of water-water cycle, decreased greatly, the accumulation of malondialdehyde (MDA) and membrane permeability increased. These results suggested that damages to peanut photosystems might be related to the accumulation of reactive oxygen species (ROS) induced by excess energy, and the water-water cycle could not dissipate energy efficiently under the stress of CH and SH, which caused the accumulation of ROS greatly. CH and SH had similar damaging effects on peanut photosystems, except that CH has more severe effects. All the results showed that CH- and SH stress has similar damaging site and mechanisms in peanut leaves.
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Abbreviations
- APX:
-
ascorbate peroxidase
- CH:
-
chilling temperature accompanied by high irradiance
- CK:
-
control plants
- Fm :
-
maximum yield of fluorescence
- Fm 0 :
-
maximum yield of fluorescence measured after dark adaptation for more than 2 h at room temperature
- Fm′:
-
the maximum yield of fluorescence in light-acclimated leaves
- Fo :
-
initial fluorescence
- Fo′:
-
the initial fluorescence in light-adapted leaves
- Fv :
-
variable fluorescence
- Fv/Fm :
-
the maximal photochemical efficiency of PSII
- Fs :
-
the steady-state fluorescence yield
- MDA:
-
malondialdehyde
- NH:
-
high-irradiance stress at room temperature
- NPQ:
-
nonphotochemical quenching
- OEC:
-
oxygen-evolving complex
- PFD:
-
photon flux density
- PSI:
-
photosystem I
- PSII:
-
photosystem II
- qP :
-
photochemical quenching
- ROS:
-
reactive oxygen species
- SH:
-
salt stress accompanied by high irradiance
- SOD:
-
superoxide dismutase
- TBA:
-
thiobarbituric acid
- TCA:
-
trichloroacetic acid
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Acknowledgements
This research was supported by the National Supporting Program of Science and Technology (2006BAD21B04), the Natural Science Foundation of Shandong Province (2009ZRC02012), the Project of Seed Industry in Shandong Province, the Special Funds for Postdoctoral Innovation of Shandong Province (200802005) and the Youth Research Foundation of Shandong Academy of Agricultural Sciences.
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Qin, L.Q., Li, L., Bi, C. et al. Damaging mechanisms of chilling- and salt stress to Arachis hypogaea L. leaves. Photosynthetica 49, 37–42 (2011). https://doi.org/10.1007/s11099-011-0005-3
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DOI: https://doi.org/10.1007/s11099-011-0005-3