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Induction by drought of crassulacean acid metabolism in the terrestrial bromeliad, Puya floccosa

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Photosynthetica

Abstract

In the terrestrial bromeliad, Puya floccosa, a value of carbon isotopic composition (δ13C) of −22‰ has been previously reported, suggesting the operation of weak and/or intermediate (C3-CAM) crassulacean acid metabolism (CAM). In order to characterize the operation of CAM in P. floccosa and its possible induction by drought, plants were grown in Caracas and subjected to four independent drought cycles. Additionally, since plants of this species grow in Venezuela in a large range of elevations, leaf samples were collected at elevations ranging from 725 to 2,100 m a.s.l. in the Venezuelan Andes and the Coastal Range, in order to evaluate the effect of elevation on CAM performance. Even though nocturnal acid accumulation occurred in both watered and droughted plants, mean ΔH+ was higher in droughted than watered plants [ΔH+ = 60.17.5 and 22.9 ± 5.2 μmol g−1(FM), respectively]. The majority of plants from all the natural populations sampled had low values of δ13C not differing significantly from those of C3 plants collected as standards and δ13C did not change with elevation. We conclude that P. floccosa is capable of a weak CAM activity, with a large variability among populations and drought experiments probably due to local and temporal differences in microclimatic variables and drought stress; elevation bears no influence on values of δ13C in this species.

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Abbreviations

CAM:

crassulacean acid metabolism

Chl:

chlorophyll

DM:

dry mass

FM:

fresh mass

FM/A:

fresh mass per area

RH:

relative humidity

T:

air temperature

Sm :

mesophyll succulence index

TR:

total radiation

TRint :

daily integrated TR

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Acknowledgements

Determinations of δ13C were kindly performed by J.P. Ometto and L. Martinelli at Centro de Energia Nuclear na Agricultura, Universidad de Sâo Paulo, Laboratório de Ecología Isotópica, Piracicaba, Brasil and E. Montes, Paleolab, University of Massachussets. Field trips were partly financed by Posgrado de Botánica, Universidad Central de Venezuela. D. Núñez did many of the acid titrations.

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Authors and Affiliations

  1. Instituto de Biología Experimental, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela

    A. Herrera, W. Tezara & C. Ballestrini

  2. Instituto Venezolano de Investigaciones Científicas, Altos de Pipe, Venezuela

    E. Medina

  3. University of Kansas, Lawrence, Kansas, USA

    C. E. Martin

Authors
  1. A. Herrera
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  2. C. E. Martin
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  3. W. Tezara
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  4. C. Ballestrini
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  5. E. Medina
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Correspondence to A. Herrera.

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Herrera, A., Martin, C.E., Tezara, W. et al. Induction by drought of crassulacean acid metabolism in the terrestrial bromeliad, Puya floccosa . Photosynthetica 48, 383–388 (2010). https://doi.org/10.1007/s11099-010-0050-3

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  • DOI: https://doi.org/10.1007/s11099-010-0050-3

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