Abstract
Maize plant inbred lines, one Al-sensitive (B-73) and two Al-tolerant (F-2 and L-2039), were grown hydroponically in the presence of 200 µM Al. After 13 d of growth, root and shoot lengths, photosystem 2 (PS2) activity, chlorophyll (Chl) content, 5-aminolevulinic acid (5-ALA) synthesis rate, chlorophyllase (Chlase) activity, and N, Mg, Fe, and Mn contents in leaves were determined. PS2 activity and Chl content were most severely affected by Al in B-73, but F-2 was almost unaffected. This was in accordance with Al-accumulation in the plants. The observed changes in B-73 coincided with 5-ALA synthesis inhibition, Chlase activation, and leaf deprivation of Fe and Mg. In Al-treated L-2039 plants, the leaf Mg and Mn contents were decreased. Also, an excessive Chlase activation was found in Al-treated L-2039, without a substantial Chl loss. This may indicate the activation of different enzyme pools in tolerant and sensitive genotypes under low-stress conditions.
References
Abdel-Basset, R., Issa, A.A., Adams, M.S.: Chlorophyllase activity: effects of heavy metals and calcium.-Photosynthetica 31: 421–425, 1995.
Ali, B., Hasan, S.A., Hayat, S., Hayat, Q., Yadav, S., Fariduddin, Q., Ahmad, A.: A role for brassinosteroids in the amelioration of aluminium stress through antioxidant system in mung bean.-Environ. exp. Bot. 62: 153–159, 2008.
Averina, N.G., Shalygo, N.V., Yaronskaya, E.B.: Effect of glutamic acid and 1,10-phenanthroline on the accumulation of chlorophyll precursors in green Phaseolus leaves.-Photosynthetica 23: 383–385, 1989.
Barcelo, J., Poschenrieder, C.: Fast root growth responses, root exudates and internal detoxification as clues to the mechanisms of aluminium toxicity and resistance: a review.-Environ. exp. Bot. 48: 75–92, 2002.
Darko, E., Ambrus, H., StefanovitsBanyai, E., Fodor, J., Bakos, F., Barnabo, B.: Aluminium toxicity, Al tolerance and oxidative stress in Al-sensitive wheat genotype and in Al-tolerant lines developed by in vitro microspore selection.-Plant Sci. 166: 583–591, 2004.
Dei, M., Tsuji, H.: Synthesis of 5-aminolevulinic acid by plastids isolated from cotyledons of greening cucumber seedlings pretreated with benzyl adenine.-Plant Physiol. 6: 63–68, 1987.
Ellsworth, R.K.: Studies on chlorophyllase. I. Hydrolytic and esterification activities of chlorophyllase from wheat seedlings.-Photosynthetica 5: 226–232, 1971.
Ellsworth, R.K., Tsuk, R.M., St. Pierre, L.A.: Studies on chlorophyllase. IV. Attribution of hydrolytic and esterifying “chlorophyllase” activities observed in vitro to two enzymes.-Photosynthetica 10: 312–323, 1976.
Garcia, A.L., Galindo, L.: Chlorophyllase activity as biochemical indicator of Mn and Fe deficiencies in Citrus.-Photosynthetica 25: 351–357, 1991.
Hadzi-Taskovic-Sukalovic, V.: Nitrogen assimilating enzyme activities in maize in response to the supply of different nitrogen sources.-J. Sci. Agr. Res. (Belgrade) 57: 43–54, 1993.
Haug, A.: Molecular aspects of aluminum toxicity.-CRC crit. Rev. Plant Sci. 1: 345–373, 1984.
Hoddinott, J., Richter, C.: The influence of aluminum on photosynthesis and translocation in French beans.-J. Plant Nutr. 10: 443–454, 1987.
Horton, P., Ruben, A.V., Walters, R.G.: Regulation of light-harvesting in green plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 655–684, 1996.
Kannangara, C.G., Gough, S.P., Hansen, B., Rasmussen, J.N., Simpson, D.J.: A homogenizer with replaceable razor blades for bulk isolation of active barley plastids.-Carlsberg Res. Commun. 42: 431–439, 1977.
Kochian, L.V.: Cellular mechanisms of aluminum toxicity and resistance in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 46: 237–260, 1995.
Kumar, A.M., Schaub, U., Söll, D., Ujwal, M.L.: Glutamyl-transfer RNA: at the crossroad between chlorophyll and protein biosynthesis.-Trends Plant Sci. 1: 371–376, 1996.
Lazic-Jancic, V., Hadzi-Taskovic-Sukalovic, V., Vuletic, M., Denic, M., Neyra, C.: Genetic variability of aluminum stress tolerance in maize.-Genetika (Belgrade) 23: 1–14, 1991.
Lazof, D.B., Goldsmith, J.G., Linton, R.W.: Aluminium, plant growth and crop productivity.-Progr. Bot. 58: 112–149, 1997.
Lewandowska, M., Hart, J.W., Jarvis, P.G.: Photosynthetic electron transport in plants of Sitka spruce subjected to differing light environments during growth.-Physiol. Plant. 37: 269–274, 1976.
Meriga, B., Reddy, B.K., Rao, K.R., Reddy, L.A., Kishor, P.B.K.: Aluminum-induced production of oxygene radicals, lipid peroxidation and DNA damage in seedlings of rice (Oryza sativa).-J. Plant Physiol. 161: 63–68, 2004.
Mihailovic, N., Lazarevic, M., Dzeletovic, Z., Vuckovic, M., Djurdjevic, M.: Chlorophyllase activity in wheat, Triticum aestivum L., leaves during drought and its dependence on the nitrogen ion form applied.-Plant Sci. 129: 141–146, 1997.
Milivojevič, D., Rastovič, A.: [Ultrastructure and photosynthetic activity of chloroplasts in manganese-nd iron-deficient maize leaves.]-Arhiv poljopriv. Nauke (Belgrade) 44: 43–49, 1983. [In Serbian.]
Morsch, V.M., Schetinger, M.R.C., Martins, A.F., Rocha, J.B.T.: Effects of cadmium, lead, mercury and zinc on δ-aminolevulinic acid dehydratase activity from radish leaves.-Biol. Plant. 45: 85–89, 2002.
Moustakas, M., Ouzounidou, G., Eleftheriou, E.P., Lannoye, R.: Indirect effects of aluminum stress on the function of photosynthetic apparatus.-Plant Physiol. Biochem. 34: 553–560, 1996.
Neumann, G., Römheld, V.: The release of root exudates as affected by the plant’s physiological status.-In: Pinton, R., Varanini, Z., Nannipieri, Z. (ed.): Biochemistry and Organic Substances at the Soil-Plant Interface. Pp. 41–94. Marcel Dekker, New York 2000.
Nichol, B.E., Oliveira, L.A., Glass, A.D.M., Siddiqi, M.Y.: The effects of aluminum on the influx of calcium, potassium, ammonium, nitrate and phosphate in an aluminum sensitive cultivar of barley (Hordeum vulgare L.).-Plant Physiol. 101: 1263–1266, 1993.
Ohki, K.: Photosynthesis, chlorophyll, and transpiration responses in aluminium stressed wheat and sorghum.-Crop Sci. 26: 572–575, 1986.
Padmaja, K., Prasad, D.D.K., Prasad, A.R.K.: Inhibition of chlorophyll synthesis in Phaseolus vulgaris L. seedlings by cadmium acetate.-Photosynthetica 24: 399–405, 1990.
Pereira, L.B., Tabaldi, L.A., Goncalves, J.F., Jucoski, G.O., Paulette, M.M., Weis, S.N., Nicoloso, F.T., Borher, D., Rocha, J.B.T., Schetinger, M.R.C.: Effect of aluminum on δ-aminolevulinic acid dehydratase (ALA-D) and the development of cucumber (Cucumis sativus).-Environ. exp. Bot. 57: 106–115, 2006.
Taylor, G.J.: Current views of the aluminum stress response; the physiological basis of tolerance.-Cur. Top. Plant Biochem. Physiol. 10: 57–93, 1991.
Tsuchiya, T., Ohta, H., Masuda, T., Mikami, B., Kita, N., Shioi, Y., Takamiya, K.: Purification and characterizyation of two isozymes of chlorophyllase from mature leaves of Chenopodium album.-Plant Cell Physiol. 38: 1026–1031, 1997.
Vernon, L.P.: Spectrophotometric determination of chlorophylls and phaeophytins in plant extracts.-Anal. Chem. 32: 1144–1150, 1960.
Vicherková, M., Minář, J.: Aluminum induced changes in growth and mineral nutrient content of maize (Zea mays L.).-Scrip. Fac. Sci. nat. Univ. Purkyně brun. 17: 133–142, 1987.
Zhang, X.-B., Peng, L., Yang, Y.S., Xu, G.-D.: Effect of Al in soil on photosynthesis and related morphological and physiological characteristics of two soybean genotypes.-Bot. Stud. 48: 435–444, 2007.
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Mihailovic, N., Drazic, G. & Vucinic, Z. Effects of aluminium on photosynthetic performance in Al-sensitive and Al-tolerant maize inbred lines. Photosynthetica 46, 476–480 (2008). https://doi.org/10.1007/s11099-008-0082-0
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DOI: https://doi.org/10.1007/s11099-008-0082-0