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Role of fructose-1,6-bisphosphatase, fructose phosphotransferase, and phosphofructokinase in saccharide metabolism of four C3 grassland species under elevated CO2

  • Original Papers
  • Published:
Photosynthetica

Abstract

We studied the effects of 15-months of elevated (700 µmol mol−1) CO2 concentration (EC) on the CO2 assimilation rate, saccharide content, and the activity of key enzymes in the regulation of saccharide metabolism (glycolysis and gluconeogenesis) of four C3 perennial temperate grassland species, the dicots Filipendula vulgaris and Salvia nemorosa and the monocots Festuca rupicola and Dactylis glomerata. The acclimation of photosynthesis to EC was downward in F. rupicola and D. glomerata whereas it was upward in F. vulgaris and S. nemorosa. At EC, F. rupicola and F. vulgaris leaves accumulated starch while soluble sugar contents were higher in F. vulgaris and D. glomerata. EC decreased pyrophosphate-D-fructose-6-phosphate l-phosphotransferase (PFP, EC 2.7.1.90) activity assayed with Fru-2,6-P2 in F. vulgaris and D. glomerata and increased it in F. rupicola and S. nemorosa. Growth in EC decreased phosphofructokinase (PFK, EC 2.7.1.11) activity in all four species, the decrease being smallest in S. nemorosa and greatest in F. rupicola. With Fru-2,6-P2 in the assay medium, EC increased the PFP/PFK ratio, except in F. vulgaris. Cytosolic fructose-1,6-bisphosphatase (Fru-1,6-P2ase, EC 3.1.3.11) was inhibited by EC, the effect being greatest in F. vulgaris and smallest in F. rupicola. Glucose-6-phosphate dehydrogenase (G6PDH EC 1.1.1.49) activity was decreased by growth EC in the four species. Activity ratios of Fru-1,6-P2ase to PFP and PFK suggest that EC may shift sugar metabolism towards glycolysis in the dicots.

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Abbreviations

AC:

ambient CO2 concentration

EC:

elevated CO2 concentration

Fru-6-P:

fructose-6-phosphate

Fru-1,6-P2 :

fructose-1,6-bisphosphate

Fru-1,6-P2ase:

fructose-1,6-bisphosphatase

Fru-2,6-P2 :

fructose-2,6-bisphosphate

G6PDH:

glucose-6-phosphate dehydrogenase

OPPP:

oxidative pentose phosphate pathway

P N :

net photosynthetic rate

PFP:

pyrophosphate-D-fructose-6-phosphate 1-phosphotransferase

PFK:

phosphofructokinase

PPFD:

photosynthetic photon flux density

PPi:

pyrophosphate

RuBPCO:

ribulose-1,5-bisphosphate carboxylase/oxygenase

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Authors and Affiliations

  1. Biological Department, College of Nyíregyháza, H-4400, Nyíregyháza, Sóstói út 31-b, Hungary

    E. Nádas, Á. Balogh & F. Kiss

  2. Institute of Botany and Ecophysiology, Faculty of Agriculture and Environmental Sciences, Hungary

    K. Szente, Z. Nagy & Z. Tuba

  3. Plant Ecological Research Group of Hungarian Academy of Sciences, Szent István University, H-2103, Gödöllő, Páter K. 1, Hungary

    Z. Tuba

  4. Institute of Natural Resources and Agricultural Biology of Salamanca, CSIC, Apartado 257, 37071, Salamanca, Spain

    R. Martínez-Carrasco

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  1. E. Nádas
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  2. Á. Balogh
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  3. F. Kiss
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  4. K. Szente
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  5. Z. Nagy
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  6. R. Martínez-Carrasco
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  7. Z. Tuba
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Correspondence to Á. Balogh.

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Nádas, E., Balogh, Á., Kiss, F. et al. Role of fructose-1,6-bisphosphatase, fructose phosphotransferase, and phosphofructokinase in saccharide metabolism of four C3 grassland species under elevated CO2 . Photosynthetica 46, 255–261 (2008). https://doi.org/10.1007/s11099-008-0042-8

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  • DOI: https://doi.org/10.1007/s11099-008-0042-8

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