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Duration of irradiation rather than quantity and frequency of high irradiance inhibits photosynthetic processes in the lichen Lasallia pustulata

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Photosynthetica

Abstract

Lichen thalli were exposed to 4 regimes differing in irradiance and duration of irradiation. Photosynthetic efficiency of thalli was monitored by chlorophyll fluorescence parameters and xanthophyll cycle analysis. Maximal quantum yield of photosystem 2 (FV/FM) decreased gradually with time in long-term treatment. The effect of additional short-term high irradiance (HI) treatment applied each 24 h was not significant. Nevertheless, short-term HI applied repeatedly on thalli kept in the dark led to a significant decrease of FV/FM. Non-photochemical quenching recorded during the long-term treatment corresponded to the content of zeaxanthin (Z). In short-term treatment, however, proportion of Z (and antheraxanthin) to total amount of xanthophyll cycle pigments recovered to the initial values every 24 h after each repeated short-term HI event in thalli kept in dark. Thus duration of irradiation rather than irradiance and frequency of HI events is important for a decrease in primary photosynthetic processes in wet thalli of Lasallia pustulata. Rapidly responding photoprotective mechanisms, such as conversion of xanthophyll cycle pigments, are involved mainly in short-term irradiation events, even at HI.

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Abbreviations

A:

antheraxanthin

CMI:

continuous medium irradiance

Chl:

chlorophyll

DEPS:

de-epoxidation state of xanthophyll cycle pigments

DM:

dry mass

FV/FM :

dark-adapted maximal quantum yield

HI:

high irradiance

LHC:

light-harvesting complex

MI:

medium irradiance

NPQ:

non-photochemical quenching

qP :

photochemical quenching

PS2:

photosystem 2

RHIM :

repetitive high irradiance applied simultaneously with continuous medium irradiance

RHID :

repetitive high irradiance applied in the dark

V:

violaxanthin

Z:

zeaxanthin

Φ2 :

quantum yield of photochemical reactions in PS2

References

  • Adams, W.W., III, Demmig-Adams, B., Lange, O.L.: Carotenoid composition and metabolism in green and blue-green-algal lichens in the field.-Oecologia 94: 576–584, 1993.

    Article  Google Scholar 

  • Barták, M, Hájek, J., Vráblíková, H., Dubová, J.: High-light stress and photoprotection in Umbilicaria antarctica monitored by chlorophyll fluorescence imaging and changes in zeaxanthin and glutathione.-Plant Biol. 6: 333–341, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Barták, M., Vráblíková, H., Hájek, J.: Sensitivity of photosystem 2 of Antarctic lichens to high irradiance stress: Fluorometric study of fruticose (Usnea antarctica) and foliose (Umbilicaria decussata) species.-Photosynthetica 41: 497–504, 2003.

    Article  Google Scholar 

  • Bilger, W., Schreiber, U.: Energy-dependent quenching of dark level chlorophyll fluorescence in intact leaves.-Photosynth. Res. 10: 303–308, 1986.

    Article  CAS  Google Scholar 

  • Brown, D.H., Hooker, T.N.: The significance of acidic lichen substances in the estimation of chlorophyll and phaeophytin in lichens.-New Phytol. 78: 617–624, 1977.

    Article  CAS  Google Scholar 

  • Calatayud, A., Deltoro, V.I., Barreno, E., del Valle-Tascon, S.: Changes in in vivo chlorophyll fluorescence quenching in lichen thalli as a function of water content and suggestion of zeaxanthin-associated photoprotection.-Physiol. Plant. 101: 93–102, 1997.

    Article  CAS  Google Scholar 

  • Demmig-Adams, B.: Carotenoids and photoprotection in plants. A role for the xanthophyll zeaxanthin.-Biochim. biophys. Acta 1020: 1–24, 1990.

    Article  CAS  Google Scholar 

  • Demmig-Adams, B., Adams, W.W., III: Xanthophyll cycle and light stress in nature: uniform response to excess direct sunlight among higher plant species.-Planta 198: 460–470, 1996.

    Article  CAS  Google Scholar 

  • Demmig-Adams, B., Adams, W.W., III, Green, T.G.A., Czygan, F.-C., Lange, O.L.: Differences in the susceptibility to light stress in two lichens forming a phycosymbiodeme, one partner possessing and one lacking the xanthophyll cycle.-Oecologia 84: 451–456, 1990a.

    Google Scholar 

  • Demmig-Adams, B., Máguas, C., Adams, W.W., III, Meyer, A., Kilian, E., Lange, O.L.: Effect of high light on the efficiency of photochemical energy conversion in a variety of lichen species with green and blue-green phycobionts.-Planta 180: 400–409, 1990b.

    Article  CAS  Google Scholar 

  • Etienne, A.-L., Kirilovsky, D.: Comparison of the primary events of photoinhibition in Cyanobacteria, green-algae and thylakoids of higher plants.-Photosynthetica 27: 85–87, 1992.

    Google Scholar 

  • Frank, H.A., Cua, A., Chynwat, V., Young, A., Gosztola, D., Wasielewski, M.R.: Photophysics of the carotenoids associated with the xanthophyll cycle in photosynthesis.-Photosynth. Res. 41: 389–395, 1994.

    Article  CAS  Google Scholar 

  • Frommolt, R., Goss, R., Wilhelm, C.: The de-epoxidase and epoxidase reactions of Mantoniella squamata (Prasinophyceae) exhibit different substrate-specific reaction kinetics compared to spinach.-Planta 213: 446–456, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Gauslaa, Y., Lie, M., Solhaug, K.A., Ohlson, M.: Growth and ecophysiological acclimation of the foliose lichen Lobaria pulmonaria in forests with contrasting light climates.-Oecologia 147: 406–416, 2006.

    Article  PubMed  Google Scholar 

  • Gauslaa, Y., McEvoy, M.: Seasonal changes in solar radiation drive acclimation of the sun-screening compound parietin in the lichen Xanthoria parietina.-Basic appl. Ecol. 6: 75–82, 2005.

    Article  CAS  Google Scholar 

  • Gauslaa, Y., Solhaug, K.A.: High-light damage in air-dry thalli of the old forest lichen Lobaria pulmonaria.-Interactions of irradiance, exposure duration and high temperature.-J. exp. Bot. 50: 697–705, 1999.

    Article  CAS  Google Scholar 

  • Gauslaa, Y., Solhaug, K.A.: High-light-intensity damage to the foliose lichen Lobaria pulmonaria within a natural forest: the applicability of chlorophyll fluorescence methods.-Lichenologist 32: 271–289, 2000.

    Article  Google Scholar 

  • Gauslaa, Y., Solhaug, K.A.: Fungal melanins as a sun screen for symbiotic green algae in the lichen Lobaria pulmonaria.-Oecologia 126: 462–471, 2001.

    Article  Google Scholar 

  • Gauslaa, Y., Solhaug, K.A.: Photoinhibition in lichens depends on cortical characteristics and hydration.-Lichenologist 36: 133–143, 2004.

    Article  Google Scholar 

  • Gilmore, A.M.: Mechanistic aspects of xanthophyll cycle-dependent photoprotection in higher plant chloroplasts and leaves.-Physiol. Plant. 99: 197–209, 1997.

    Article  CAS  Google Scholar 

  • Gilmore, A.M., Yamamoto, H.Y.: Time-resolution of the antheraxanthin-and ΔpH-dependent chlorophyll a fluorescence components associated with photosystem II energy dissipation in Mantoniella squamata.-Photochem. Photobiol. 74: 291–302, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Havaux, M., Niyogi, K.K.: The violoxanthin cycle protects from photoxidative damage by more than one mechanism.-Proc. nat. Acad. Sci. USA 96: 8762–8767, 1999.

    Article  PubMed  CAS  Google Scholar 

  • Heber, U., Lange, O.L., Shuvalov, V.A.: Conservation and dissipation of light energy as complementary processes: homoiohydric and poikilohydric autotrophs.-J. exp. Bot. 57: 1211–1223, 2006.

    Article  PubMed  CAS  Google Scholar 

  • Horton, P., Ruban, A.V., Rees, D., Pascal, A.A., Noctor, G.D., Young, A.J.: Control of the light-harvesting function of chloroplast membrane by aggregation of the LHCII chlorophyll protein complex.-FEBS Lett. 292: 1–4, 1992.

    Article  Google Scholar 

  • Jin, E., Polle, J.E.W., Lee, H.K., Hyun, S.M., Chang, M.: Xanthophylls in microalgae: From biosynthesis to biotechnological mass production and application.-J. Microbiol. Biotechnol. 13: 165–174, 2003.

    Article  CAS  Google Scholar 

  • Kramer, D.M., Johnson, G., Kiirats, O., Edwards, G.E.: New fluorescence parameters for the determination of QA redox state and excitation energy fluxes.-Photosynth. Res. 79: 209–218, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Krause, G.H., Weis, E.: Chlorophyll fluorescence and photosynthesis-the basics.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313–349, 1991.

    Article  CAS  Google Scholar 

  • Krieger, A., Moya, I., Weis, E.: Energy-dependent quenching of chlorophyll-a fluorescence-effect of pH on stationary fluorescence and picosecond-relaxation kinetics in thylakoid membranes and Photosystem II preparations.-Biochim. biophys. Acta 1102: 167–176, 1992.

    Article  CAS  Google Scholar 

  • Lange, O.L.: Photosynthetic productivity of the epilithic lichen Lecanora muralis: Long-term field monitoring of CO2 exchange and its physiological interpretation. I. Dependence of photosynthesis on water content, light, temperature, and CO2 concentration from laboratory measurements.-Flora 197: 233–249, 2002.

    Google Scholar 

  • Lange, O.L.: Photosynthetic productivity of the epilithic lichen Lecanora muralis: Long-term field monitoring of CO2 exchange and its physiological interpretation. III. Diel, seasonal, and annual carbon budgets.-Flora 198: 277–292, 2003.

    Google Scholar 

  • Latowski, D., Kostecka-Gugala, A., Strzalka, K.: Effect of temperature on violaxanthin de-epoxidation: Comparison of the in vivo and model system.-Russ. J. Plant Physiol. 50: 173–177, 2003.

    Article  CAS  Google Scholar 

  • Leisner, J.M.R., Bilger, W., Lange, O.L.: Chlorophyll fluorescence characteristics of the cyanobacterial lichen Peltigera rufescens under field conditions. 1. Seasonal patterns of photochemical activity and the occurrence of photosystem II inhibition.-Flora 191: 261–273, 1996.

    Google Scholar 

  • Litvin, R., Bina, D., Siffel, P., Vacha, F.: Conformational changes and their role in non-radiative energy dissipation in photosystem II reaction centres.-Photochem. photobiol. Sci. 4: 999–1002, 2005.

    Article  PubMed  CAS  Google Scholar 

  • MacKenzie, T.D.B., Król, M., Huner, N.P.A., Campbell, D.A.: Seasonal changes in chlorophyll fluorescence quenching and the induction and capacity of the photoprotective xanthophyll cycle in Lobaria pulmonaria.-Can. J. Bot. 80: 255–261, 2002.

    Article  CAS  Google Scholar 

  • MacKenzie, T.D.B., MacDonald, T.M., Dubois, L.A., Campbell, D.A.: Seasonal changes in temperature and light driven acclimation of photosynthetic physiology and macromolecular content in Lobaria pulmonaria.-Planta 214: 57–66, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Masojidek, J., Kopecky, J., Koblizek, M., Torzillo, G.: The xanthophyll cycle in green algae (Chlorophyta): Its role in the photosynthetic apparatus.-Plant Biol. 6: 342–349, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Nedbal, L., Samson, G., Whitmarsh, J.: Redox state of a one-electron component controls the rate of photoinhibition of photosystem II.-Proc. nat. Acad. Sci. USA 89: 7929–7933, 1992.

    Article  PubMed  CAS  Google Scholar 

  • Pfeifhofer, H.W., Willfurth, R., Zorn, M., Kranner, I.: Analysis of chlorophylls, carotenoids, and tocopherols in lichens.-In: Kranner, I., Beckett, R.P., Varma, A. (ed.): Protocols in Lichenology. Pp. 363–378. Springer-Verlag, Berlin-Heidelberg-New York-Barcelona-Hong Kong-Milan-Paris-Tokyo 2002.

    Google Scholar 

  • Pfündel, E., Bilger, W.: Regulation and possible function of the violoxanthin cycle.-Photosynth. Res. 42: 89–109, 1994.

    Article  Google Scholar 

  • Roháček, K.: Chlorophyll fluorescence parameters: the definitions, photosynthetic meaning, and mutual relationships.-Photosynthetica 40: 13–29, 2002.

    Article  Google Scholar 

  • Schlensog, M., Schroeter, B.: A new method for the accurate in situ monitoring of chlorophyll-a-fluorescence in lichens and bryophytes.-Lichenologist 33: 443–452, 2001.

    Article  Google Scholar 

  • Schroeter, B., Kappen, L., Schulz, F., Sancho, L.G.: Seasonal variation in the carbon balance of lichens in the maritime antartic: Long-term mesurements of photosynthetic activity in Usnea aurantiaco-atra.-In: Davison, W., Howard-Williams, C., Broady, P. (ed.): Antarctic Ecosystems: Models for wider ecological understanding. Pp. 258–262. Cantebury University, New Zealand 2000.

    Google Scholar 

  • Smith, E.C., Griffiths, H.: Intraspecific variation in photosynthetic responses of trebouxioid lichens with reference to the activity of a carbon-concentrating mechanism.-Oecologia 113: 360–369, 1998.

    Article  Google Scholar 

  • Smith, V.R., Gremmen, N.J.M.: Photosynthesis in a sub-Antarctic zone lichen.-New Phytol. 149: 291–299, 2001.

    Article  Google Scholar 

  • Solhaug, K.A., Gauslaa, Y.: Parietin, a photoprotective secondary product of the lichen Xanthoria parietina.-Oecologia 108: 412–418, 1996.

    Article  Google Scholar 

  • Valladares, F., Sanchez Hoyos, A., Manrique, E.: Diurnal changes in photosynthetic efficiency and carotenoid composition of the lichen Anaptychia ciliaris-effects of hydration and light-intensity.-Bryologist 98: 375–382, 1995.

    Article  CAS  Google Scholar 

  • Vráblíková, H., Barták, M., Wönisch, A.: Changes in glutathione and xantophyll cycle pigments in the high light-stressed lichens Umbilicaria antarctica and Lasallia pustulata.-J. Photochem. Photobiol. B 79: 35–41, 2005.

    Article  PubMed  CAS  Google Scholar 

  • Vráblíková, H., McEvoy, M., Solhaug, K.A., Barták, M., Gauslaa, Y.: Annual variation in photoacclimation and photoprotection of the photobiont in the foliose lichen Xanthoria parietina.-J. Photochem. Photobiol. B 83: 151–162, 2006.

    Article  PubMed  CAS  Google Scholar 

  • Yin, Z.-H., Johnson, G.N.: Photosynthetic acclimation of higher plants to growth in fluctuating light environments.-Photosynth. Res. 63: 97–107, 2000.

    Article  PubMed  CAS  Google Scholar 

  • Zsiros, O., Allakhverdiev, S.I., Higashi, S., Watanabe, M., Nishiyama, Y., Murata, N.: Very strong UV-A light temporally separates the photoinhibition of photosystem II into light-induced inactivation and repair.-Biochim. biophys. Acta 1757: 123–129, 2006.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Plant Physiology & Anatomy, Institute of Experimental Biology, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic

    M. Barták, H. Vráblíková-Cempírková, J. Štepigová, J. Hájek, P. Váczi & K. Večeřová

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  1. M. Barták
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  2. H. Vráblíková-Cempírková
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  3. J. Štepigová
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Correspondence to H. Vráblíková-Cempírková.

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Barták, M., Vráblíková-Cempírková, H., Štepigová, J. et al. Duration of irradiation rather than quantity and frequency of high irradiance inhibits photosynthetic processes in the lichen Lasallia pustulata . Photosynthetica 46, 161–169 (2008). https://doi.org/10.1007/s11099-008-0027-7

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