Abstract
High level of phosphoenolpyruvate carboxylase (PEPC) gene was stably inherited and transferred from the male parent, PEPC transgenic rice, into a female parent, japonica rice cv. 9516. Relative to the female parent, the produced JAAS45 pollen lines exhibited high PEPC activity (17-fold increase) and also higher photosynthetic rates (about 36 %-fold increase). The JAAS45 pollen lines were more tolerant to photoinhibition and to photo-oxidative stress. Furthermore, JAAS45 pollen lines, as well as their male parent, were tested to exhibit a limiting C4 cycle by feeding with exogenous C4 primary products such as oxaloacetate (OAA). Thus the PEPC gene and photosynthetic characteristics of PEPC transgenic rice could be stably transferred to the hybrid progenies, which might open a new breeding approach to the integration of conventional hybridization and biological technology.
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Abbreviations
- Fv/Fm :
-
the maximum photochemical efficiency of photosystem 2
- HI:
-
sprayed with distilled water and exposed to high irradiance [1 400 µmol m−2 s−1]
- HI + MV:
-
sprayed with 1.5 mM methyl viologen (MV) and exposed to high irradiance [1 400 µmol m−2 s−1]
- JAAS45:
-
JAAS45 pollen line
- MV:
-
methyl viologen
- OAA:
-
oxaloacetate
- PCR:
-
polymerase chain reaction
- PC:
-
PEPC transgenic rice
- PEPC:
-
phosphoenolpyruvate carboxylase
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An erratum to this article is available at http://dx.doi.org/10.1007/s11099-007-0077-2.
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Ling, L., Zhang, B.J. & Jiao, D.M. Photosynthetic characteristics of hybrid rice with phosphoenolpyruvate carboxylase gene. Photosynthetica 45, 317–320 (2007). https://doi.org/10.1007/s11099-007-0054-9
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DOI: https://doi.org/10.1007/s11099-007-0054-9