Development of photosystems 2 and 1 during leaf growth in grapevine seedlings probed by chlorophyll a fluorescence transient and 820 nm transmission in vivo
- 231 Downloads
Chlorophyll (Chl) a fluorescence transient and 820-nm transmission kinetic were investigated to explore the development of photosynthetic apparatus in grapevine leaves from emergence to full expansion. In this study, all leaves at various developing stages exhibited typical Chl a fluorescence transient. In newly initiating leaves, the maximum quantum yield of primary photochemistry (ϕP0) was slightly lower (<10 %) than that in fully expanded leaves. Nevertheless, the fluorescence rise from O to J step was clearly speeded up in young leaves compared with that in fully expanded leaves. Additionally, a distinct K step appeared in young leaves at high irradiances. With leaf development, the efficiency that a trapped exciton can move an electron into the electron transport chain further than QA − (Ψ0), the quantum yield of electron transport beyond QA (ϕE0), electron transport flux per excited cross section (ET0/CS0), the amount of active photosystem (PS) 2 reaction centres per excited cross section (RC/CS0), and the performance index on cross section basis (PICS) increased gradually and rapidly. Young leaves had strikingly lower amplitude of transmission at 820 nm. A linear relationship between Ψ0 and the transmission at 820 nm (I30/I0) was evident. Based on these data, we suggest that (1) the primary photochemistry of PS2 may be not the limiting step of the photosynthetic capacity during leaf growth under natural irradiance; (2) oxygen evolving complex (OEC) might be not fully connected to PS2 at the beginning of leaf growth; (3) though there are a few functional PS1 and PS2 at the early stages of leaf development, they match perfectly.
Additional key wordsirradiance leaf age oxygen evolving complex performance index reaction centre Vitis
Unable to display preview. Download preview PDF.
- Franck, F., Sironval, C., Schmid, G.H.: Extension of the experiment by J.H.C. Smith on the onset of the oxygen evolution in etiolated bean leaves.-In: Sironval, C., Brouers, M. (ed.): Protochlorophyllide Reduction and Greening. Pp. 223–236. Martinus Nijhoff/Dr W. Junk Publ., The Hague-Boston-Lancaster 1984.Google Scholar
- Jiang, C.-D., Jiang, G.-M., Wang, X., Li, L.-H., Biswas, D.K., Li, Y.-G.: Enhanced photosystem 2 thermostability during leaf growth of elm Ulmus pumila seedlings.-Photosynthetica 44: 411–418, 2006.Google Scholar
- Ogawa, T., Inoue, Y., Kitajima, M., Shibata, K.: Action spectra for biosynthesis of chlorophyll a and b and β-carotene.-Photochem. Photobiol. 18: 229–235, 1973.Google Scholar
- Schreiber, U., Klughammer, C., Neubauer, C.: Measuring P700 absorbance changes around 830 nm with a new type of pulse modulation system.-Z. Naturforsch. 43c: 686–698, 1988.Google Scholar
- Srivastava, A., Strasser, R.J., Govindjee: Greening of peas: parallel measurements of 77 K emission spectra, OJIP chlorophyll a fluorescence transient, period four oscillation of the initial fluorescence level, delayed light emission, and P700.-Photosynthetica 37: 365–392, 1999.CrossRefGoogle Scholar
- Strasser, B.J., Strasser, R.J.: Measuring fast fluorescence transients to address environmental questions: The JIP-test.-In: Mathis, P. (ed.): Photosynthesis: from Light to Biosphere. Vol. V. Pp. 977–980. Kluwer Academic Publ., Dordrecht-Boston-London 1995.Google Scholar
- Strasser, R.J., Srivastava, A., Tsimilli-Michael, M.: The fluorescence transient as a tool to characterize and screen photosynthetic samples.-In: Yunus, M., Pathre, U., Mohanty, P. (ed.): Probing Photosynthesis: Mechanisms, Regulation and Adaptation. Pp. 445–483. Taylor & Francis, London-New York 2000.Google Scholar
- Strasser, R.J., Tsimilli-Michael, M.: Structure function relationship in the photosynthetic apparatus: A biophysical approach.-In: Pardha Saradhi, P. (ed.): Biophysical Processes in Living Systems. Pp. 271–303. Science Publ., Enfield 2001.Google Scholar
- Strasser, R.J., Tsimilli-Michael, M., Srivastava, A.: Analysis of the chlorophyll a fluorescence transient.-In: Papageorgiou, G.C., Govindjee (ed.): Chlorophyll a Fluorescence: A Signature of Photosynthesis. Pp. 321–362. Springer, Dordrecht 2004.Google Scholar