In order to elucidate the effects of chilling-stress at night on photosystem 2 (PS2) efficiency under dim irradiance (DI), mango leaves were chilled to varied extent (8–3 °C) and for varied duration (0–12 h) in growth cabinets in the dark, and then exposed to DI (20 μmol m−2 s−1 PPFD) at each chilling-temperature for 1 h. Chilling in the dark had little effect on Fv/Fm of mango leaves. But both the extent and duration of chilling pre-treatments significantly affected Fv’/Fm’ when leaves were exposed to DI. This down-regulation of PS2 efficiency was closely related to xanthophyll de-epoxidation, assessed as photochemical reflectance index (PRI) and calculated from leaf spectral reflectance [(R531 − R570)/(R531 + R570)], and non-photochemical quenching (NPQ). The down-regulation of PS2 is a defence mechanism initiated at predawn in winter to alleviate the damage of PS2 by the sudden and strong irradiation at sunrise. Mango leaves, transferred suddenly from warm and dark room to DI and chilling showed a slight down-regulation of PS2 efficiency, in spite of an increased xanthophyll de-epoxidation. This might have been due to the unavailability of some cofactors required for NPQ.
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- Fv/Fm :
potential efficiency of PS2
actual efficiency of PS2 under irradiation
photon flux density
photochemical reflectance index [(R531 − R570)/(R531 + R570)]
Adams, W.W., III, Demmig-Adams, B., Logan, B.A., Barker, D.H., Osmond, C.B.: Rapid changes in xanthophyll cycle-dependent energy dissipation and photosystem II efficiency in two vines, Stephania japonica and Smilax australis, growing in the understory of an open Eucalyptus forest.-Plant Cell Environ. 22: 125–136, 1999.
Adams, W.W., III, Demmig-Adams, B., Rosenstiel, T.N., Ebbert, V.: Dependence of photosynthesis and energy dissipation activity upon growth form and light environment during the winter.-Photosynth. Res. 67: 51–62, 2001.
Adams, W.W., III, Ryanzarter, C., Ebbert, V., Demmig-Adams, B.: Photoprotective strategies of overwintering evergreens.-BioSciences 54: 41–49, 2004.
Allen, D.J., Ort, D.R.: Impacts of chilling temperatures on photosynthesis in warm-climate plants.-Trends Plant Sci. 6: 36–42, 2001.
Allen, D.J., Ratner, K., Giller, Y.E., Gussakovsky, E.E., Shahak, Y., Ort, D.R.: An overnight chill induces a delayed inhibition of photosynthesis at midday in mango (Mangifera indica L.).-J. exp. Bot. 51: 1893–1902, 2000.
Bachmann, K.M., Ebbert, V., Adams, W.W., III, Verhoeven, A.S., Barry, A. Logan, B.A., Demmig-Adams, B.: Effects of lincomycin on PSII efficiency, non-photochemical quenching, D1 protein and xanthophyll cycle during photoinhibition and recovery.-Funct. Plant Biol. 31: 803–813, 2004.
Baker, N.R.: Chilling stress and photosynthesis.-In: Foyer, C.H., Mullineaux, P.M. (ed.): Cause of Photooxidative Stress and Amelioration of Defense Systems in Plants. Pp. 127–154. CRC Press, Boca Raton 1994.
Bilger, W., Björkman, O.: Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis.-Photosynth. Res. 25: 173–185, 1990.
Close, D.C., Beadle, C.L., Hovenden, M.J.: Cold-induced photoinhibition and foliar pigment dynamics of Eucalyptus nitens seedlings during establishment.-Aust. J. Plant Physiol. 28: 1133–1141, 2001.
Demmig-Adams, B., Adams, W.W., III: Photoprotection and other responses of plants to high light stress.-Annu. Rev. Plant Phys. Plant mol. Biol. 43: 599–626, 1992.
Demmig-Adams, B., Adams, W.W., III, Barker, D.H., Logan, B.A., Bowling, D.R., Verhoeven, A.S.: Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation.-Physiol. Plant. 98: 253–264, 1996.
Ensminger, I., Sveshnikov, D., Campbell, D.A., Funk, C., Jansson, S., Lloyd, J., Shibistova, O., Öquist, G.: Intermittent low temperatures constrain spring recovery of photosynthesis in boreal Scots pine forests.-Glob. Change Biol. 10: 1–14, 2004.
Gamon, J.A., Field, C.B., Bilger, W., Björkman, O., Fredeen, A.L., Peñuelas, J.: Remote sensing of the xanthophyll cycle and chlorophyll fluorescence in sunflower leaves and canopies.-Oecologia 85: 1–7, 1990.
Gamon, J.A., Field, C.B., Fredeen, A.L., Thayer, S.: Assessing photosynthetic downregulation in sunflower stands with an optically-based model.-Photosynth. Res. 67: 113–125, 2001.
Gamon, J.A., Filella, I., Peñuelas, J.: The dynamic 531-nanometer Δreflectance signal: A survey of twenty angiosperm species.-In: Yamamoto, H.Y., Smith, C.M. (ed.): Photosynthetic Responses to the Environment. Pp. 127–177. American Society of Plant Physiologists, Rockville 1993.
Gamon, J.A., Peñuelas, J., Field, C.B.: A narrow-waveband spectral index that tracks diurnal changes in photosynthetic efficiency.-Remote Sens. Environ. 41: 35–44, 1992.
Gamon, J.A., Serrano, L., Surfus, J.S.: The photochemical reflectance index: an optical indicator of photosynthetic radiation use efficiency across species, functional types, and nutrient levels.-Oecologia 112: 492–501, 1997.
Gamon, J.A., Surfus, J.S.: Assessing leaf pigment content and activity with a reflectometer.-New Phytol. 143: 105–117, 1999.
Guo, J., Trotter, C.M.: Estimating photosynthetic light-use efficiency using the photochemical reflectance index: variations among species.-Funct. Plant Biol. 31: 255–265, 2004.
Guo, Y.-H., Cao, K.-F.: Effect of night chilling on photosynthesis of two coffee species grown under different irradiances.-J. hortic. Sci. Biotechnol. 79: 713–716, 2004.
Hikosaka, K., Kato, M.C., Hirose, T.: Photosynthetic rates and partitioning of absorbed light energy in photoinhibited leaves.-Physiol. Plant. 121: 699–708, 2004.
Holt, N.E., Zigmantas, D., Valkunas, L., Li, X.P., Niyogi, K.K., Fleming, G.R.: Carotenoid cation formation and the regulation of photosynthetic light harvesting.-Science 307: 433–436, 2005.
Külheim, C., Ågren, J., Jansson, S.: Rapid regulation of light harvesting and plant fitness in the field.-Science 297: 91–93, 2002.
Leegood, R.C.: Effects of temperature on photosynthesis and photorespiration.-In: Smirnoff, N. (ed.): Environment and Plant Metabolism. Flexibility and Acclimation. Pp. 45–62. Bios Sci. Publ., Oxford 1995.
Li, X.-G., Wang, X.-M., Meng, Q.-W., Zou, Q.: Factors liming photosynthetic recovery in sweet pepper leaves after short-term chilling stress under low irradiance.-Photosynthetica 42: 257–262, 2004.
Li, X.P., Björkman, O., Shih, C., Grossman, A.R., Rosenquist, M., Jansson, S., Niyogi, K.K.: A pigment-binding protein essential for regulation of photosynthetic light harvesting.-Nature 403: 391–395, 2000.
Lidon, F.C., Loureiro, A.S., Vieira, D.E., Bilhó, E.A., Nobre, P., Costa, R.: Photoinhibition in chilling stressed wheat and maize.-Photosynthetica 39: 161–166, 2001.
Long, S.P., Humphries, S., Falkowski, P.G.: Photoinhibition of photosynthesis in nature.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 633–662, 1994.
Morosinotto, T., Caffarri, S., Dall’Osto, L., Bassi, R.: Mechanistic aspects of the xanthophyll dynamics in higher plant thylakoids.-Physiol. Plant. 119: 347–354, 2001.
Müller, P., Li, X.-P., Niyogi, K.K.: Non-photochemical quenching. A response to excess light energy.-Plant Physiol. 125: 1558–1566, 2001.
Osmond, C.B., Grace, S.C.: Perspectives on photoinhibition and photorespiration in the field: quintessential inefficiencies of the light and dark reactions of photosynthesis?-J. exp. Bot. 46: 1351–1362, 1995.
Peñuelas, J., Gamon, J.A., Fredeen, A.L., Merino, J., Field, C.B.: Reflectance indices associated with physiological changes in nitrogen-and water-limited sunflower leaves.-Remote Sens. Environ. 48: 135–146, 1994.
Stylinski, C.D., Gamon, J.A., Oechel, W.C.: Seasonal patterns of reflectance indices, carotenoid pigments and photosynthesis of evergreen chaparral species.-Oecologia 131: 366–374, 2002.
Szalai, G., Janda, T., Páldi, E., Szigeti, Z.: Role of light in the development of post-chilling symptoms in maize.-J. Plant Physiol. 148: 378–383, 1996.
Tsonev, T., Velikova, V., Georgieva, K., Hyde, P.F., Jones, H.G.: Low temperature enhances photosynthetic down-regulation in French bean (Phaseolus vulgaris L.) plants.-Ann. Bot. 91: 343–352, 2003.
Verhoeven, A.S., Adams, W.W., III, Demmig-Adams, B.: Close relationship between the state of the xanthophyll cycle pigments and photosystem II efficiency during recovery from winter stress.-Physiol. Plant. 96: 567–576, 1996.
Verhoeven, A.S., Adams, W.W., III, Demmig-Adams, B.: Two forms of sustained xanthophylls cycle-dependent energy dissipation in overwintering Euonymus kiautschovicus.-Plant Cell Environ. 21: 893–903, 1998.
Watson, T.L., Close, D.C., Davidson, N.J., Davies, N.W.: Pigment dynamics during cold-induced photoinhibition of Acacia melanoxylon.-Funct. Plant Biol. 31: 481–489, 2004.
Xu, C.C., Li, L., Kuang, T.: Photoprotection in chilling-sensitive and-resistant plants illuminated at a chilling temperature: role of the xanthophyll cycle in the protection against lumen acidification.-Aust. J. Plant Physiol. 27: 239–244, 2004.
Ying, J., Lee, E.A., Tollenaar, M.: Response of leaf photosynthesis during the grain-filling period of maize to duration of cold exposure, acclimation, and incident PPFD.-Crop Sci. 42: 1164–1172, 2002.
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Weng, J.H., Jhaung, L.H., Jiang, J.Y. et al. Down-regulation of photosystem 2 efficiency and spectral reflectance in mango leaves under very low irradiance and varied chilling treatments. Photosynthetica 44, 248–254 (2006). https://doi.org/10.1007/s11099-006-0015-8
Additional key words
- chlorophyll fluorescence
- dim irradiance
- leaf spectral reflectance
- low temperature
- Mangifera indica