Abstract
Relationship of leaf anatomy with photosynthetic acclimation of Valeriana jatamansi was studied under full irradiance [FI, 1 600 μmol(PPFD) m−2 s−1] and net-shade [NS, 650 μmol(PPFD) m−2 s−1]. FI plants had thicker leaves with higher respiration rate (R D), nitrogen content per unit leaf area, chlorophyll a/b ratio, high leaf mass per leaf area unit (LMA), and surface area of mesophyll cell (S mes) and chloroplasts (S c) facing intercellular space than NS plants. The difference between leaf thickness of FI and NS leaves was about 28 % but difference in photon-saturated rate of photosynthesis per unit leaf area (P Nmax) was 50 %. This indicates that P Nmax can increase to a larger extent than the leaf thickness with increasing irradiance in V. jatamansi. Anatomical studies showed that the mesophyll cells of FI plants had no open spaces along the mesophyll cell walls (higher S c), but in NS plants wide open spaces along the mesophyll cell wall (lower S c) were found. Positive correlation between S c and P Nmax explained the higher P Nmax in FI plants. Increase in mesophyll thickness increased the availability of space along the mesophyll cell wall for chloroplasts (increased S c) and hence P Nmax was higher in FI plants. Thus this Himalayan species can acclimate to full sunlight by altering leaf anatomy and therefore may be cultivated in open fields.
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Abbreviations
- C i :
-
intercellular CO2 concentration
- Chl:
-
chlorophyll
- LMA:
-
leaf mass per leaf area unit
- P Nmax :
-
net photosynthetic rate per leaf area at saturating irradiance
- PPFD:
-
photosynthetic photon flux density
- Sc :
-
surface area of chloroplasts facing intercellular space
- Smes :
-
surface area of mesophyll cell facing intercellular space
References
Anonymous: The Wealth of India. A Dictionary of Indian Raw Materials and Industrial Products. Vol. X. — Publication and Information Directorate, CSIR, New Delhi 1976.
Anten, N.P.R., Werger, M.J.A.: Canopy structure and nitrogen distribution in dominant and subordinate plants in a dense stand of Amaranthus dubius L. with a size hierarchy of individuals. — Oecologia 105: 30–37, 1996.
Björkman, O.: Responses to different quantum flux densities. — In: Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H. (ed.): Physiological Plant Ecology I. Pp. 57–107. Springer-Verlag, Berlin — Heidelberg — New York 1981.
Boardman, N.K.: Comparative photosynthesis of sun and shade plants. — Annu. Rev. Plant Physiol. 28: 355–377, 1977.
Chazdon, R.L., Kaufmann, S.: Plasticity of leaf anatomy of two forest shrubs in relation to photosynthetic irradiance acclimation. — Funct. Ecol. 7: 385–394, 1993.
Concon, J.M., Soltess, D.: Rapid micro Kjeldahl digestion of cereal grains and other biological materials. — Anal. Biochem. 53: 35–41, 1973.
Evans, J.R.: Partitioning of nitrogen between and within leaves grown under different irradiance. — Aust. J. Plant Physiol. 16: 533–548, 1989.
Evans, J.R., Caemmerer, S. von, Setchell, B.A., Hudson, G.S.: The relationship between CO2 transfer conductance and leaf anatomy in transgenic tobacco with a reduced content of Rubisco. — Aust. J. Plant Physiol. 21: 475–495, 1994.
Foss, R., Houghton, P.J.: Valeriana products. — In: Houghton, P.J. (ed.): Valerian, the Genus Valeriana. Pp. 129–137. Harwood Academic Publ., Amsterdam 1997.
Frak, E., Le Roux, X., Millard, P., Dreyer, E., Jaouen, G., Saint-Joanis, B., Wendler, R.: Changes in total leaf nitrogen and partitioning of leaf nitrogen drive photosynthetic acclimation to light in fully developed walnut leaves. — Plant Cell Environ. 24: 1279–1288, 2001.
Genty, B., Harbinson, J.: Regulation of light utilisation for photosynthetic electron transport. — In: Baker, N.R. (ed.): Photosynthesis and the Environment. Pp. 67–99. Kluwer Academic Publ., Dordrecht — Boston — London 1996.
Givnish, T.J.: Adaptation to sun and shade: a whole-plant perspective. — Aust. J. Plant Physiol. 15: 63–92, 1988.
Grime, J.P.: Shade tolerance in flowering plants. — Nature 208: 161, 1965.
Hikosaka, K., Terashima, I.: A model of the acclimation of photosynthesis in the leaves of C3 plants to sun and shade with respect to nitrogen use. — Plant Cell Environ. 18: 605–618, 1995.
Honda, S.I., Hongladarom-Honda, T., Kwanyuen, P., Wildman, S.G.: Interpretations on chloroplast reproduction derived from correlation between cells and chloroplasts. — Planta 97: 1–15, 1971.
Kaul, M.K., Handa, S.: Response of medicinal plants to changed habitats and altitudes. — J. trop. med. Plants 1: 125–137, 2000.
Lusk, C.H., Reich, P.B.: Relationship of leaf dark respiration with light environment and tissue nitrogen content in juveniles of 11 cold-temperate tree species. — Oecologia 123: 318–329, 2000.
Mohotti, A.J., Lawlor, D.W.: Diurnal variation of photosynthesis and photoinhibition in tea: effects of irradiance and nitrogen supply during growth in the field. — J. exp. Bot. 53: 313–322, 2002.
Mooney, H.A., Gulmon, G.L.: Environmental and evolutionary constraints on the photosynthetic characteristics of higher plants. — In: Solbrig, O.T., Jain, S., Johnson, G.B., Raven, P.H. (ed.): Topics in Plant Population Biology. Pp. 316–337. Columbia University Press, New York 1979.
Niinemets, Ü., Tenhunen, J.D.: A model separating structural and physiological effects on carbon gain along light gradients for the shade-tolerant species Acer saccharum. — Plant Cell Environ. 20: 845–866, 1997.
Nishio, J.N., Sun, J., Vogelmann, T.C.: Photoinhibition and the light environment within leaves. — In: Baker, N.R., Bowyer, J.R. (ed.): Photoinhibition of Photosynthesis from Molecular Mechanisms to the Field. Pp. 221–237. Bios Scientific Publ., Oxford 1994.
Nobel, P.S.: Physicochemical and Environmental Plant Physiology. — Academic Press, San Diego 1991.
Oguchi, R., Hikosaka, K., Hirose, T.: Does the photosynthetic light-acclimation need changes in leaf anatomy? — Plant Cell Environ. 26: 505–512, 2003.
Pandey, S., Kumar, S., Nagar, P.K.: Photosynthetic performance of Ginkgo biloba L. grown under high and low irradiance. — Photosynthetica 41: 505–511, 2003.
Pandey, S., Misra, S.K., Kumar, N.: Post harvest change in excised Piper betle L. leaf: Temporal changes in betel types bangle and kapoori. — Indian J. exp. Biol. 36: 95–98, 1998.
Pandey, S., Nagar, P.K.: Leaf surface wetness and morphological characteristics of Valeriana jatamansi grown under open and shade habitats. — Biol. Plant. 45: 291–294, 2002.
Pons, T.L., Vanrijnberk, H., Scheurwater, I., Vanderwerf, A.: Importance of the gradient in photosynthetically active radiation in a vegetation stand for leaf nitrogen allocation in two monocotyledons. — Oecologia 95: 416–424, 1993.
Ramalho, J.C., Pons, T.L., Groenveld, H.W., Nunes, M.A.: Photosynthetic responses of Coffea arabica leaves to a short-term high light exposure in relation to N availability. — Physiol. Plant. 101: 229–239, 1997.
Schiefthaler, U., Russell, A.W., Bolhàr-Nordenkampf, H.R., Critchley, C.: Photoregulation and photodamage in Schefflera arboricola leaves adapted to different light environments. — Aust. J. Plant Physiol. 26: 485–494, 1997.
Sims, D.A., Pearcy, R.W.: Response of leaf anatomy and photosynthetic capacity in Alocasia-macrorrhiza Araceae to a transfer from low to high light. — Amer. J. Bot. 79: 449–455, 1992.
Sinoquet, H., le Roux, X., Adam, B., Ameglio, T., Daudet, F.A.: RATP: a model for simulating the spatial distribution of radiation absorption, transpiration and photosynthesis within canopies: application to an isolated tree crown. — Plant Cell Environ. 24: 395–406, 2001.
Syvertsen, J.P., Lloyd, J., McConchie, C., Kriedemann, P.E., Farquhar, G.D.: On the relationship between leaf anatomy and CO2 diffusion through the mesophyll of hypostomatous leaves. — Plant Cell Environ. 18: 149–157, 1995.
Terashima, I., Miyazawa, S.I., Hanba, Y.T.: Why are sun leaves thicker than shade leaves? Consideration based on analyses of CO2 diffusion in the leaf. — J. Plant Res. 114: 93–105, 2001.
Thain, J.F.: Curvature correction factors in the measurement of cell surface areas in plant tissues. — J. exp. Bot. 34: 87–94, 1983.
Taiz, L., Zeiger, E.: Plant Physiology. — Sinauer, Sunderland 1998.
Vats, S.K., Pandey, S., Nagar, P.K.: Photosynthetic response to irradiance in Valeriana jatamansi Jones, a threatened understorey medicinal herb of Western Himalaya. — Photosynthetica 40: 625–628, 2002.
Yamashita, N., Ishida, A., Kushima, H., Tanaka, N.: Acclimation to sudden increase in light favoring an invasive over native trees in subtropical islands, Japan. — Oecologia 125: 412–419, 2000.
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Pandey, S., Kushwaha, R. Leaf anatomy and photosynthetic acclimation in Valeriana jatamansi L. grown under high and low irradiance. Photosynthetica 43, 85–90 (2005). https://doi.org/10.1007/s11099-005-5090-8
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DOI: https://doi.org/10.1007/s11099-005-5090-8