Skip to main content
SpringerLink
Log in

Photoinhibition of photosynthesis in leaves of grapevine (Vitis vinifera L. cv. Riesling). Effect of chilling nights

  • Published:
Photosynthetica

Abstract

Photoinhibition of photosynthesis was investigated in control (C) and chilling night (CN) leaves of grapevine under natural photoperiod at different sampling time in a day. The degree of photoinhibition was determined by means of the ratio of variable to maximum chlorophyll fluorescence (Fv/Fm) and photosynthetic electron transport measurements. When the potential efficiency of photosystem (PS) 2, Fv/Fm was measured at midday, it markedly declined with significant increase of F0 in CN leaves. In isolated thylakoids, the rate of whole chain and PS2 activity were markedly decreased in CN leaves than control leaves at midday. A smaller inhibition of PS1 activity was also observed in both leaf types. Later, the leaves reached maximum PS2 efficiencies similar to those observed in the morning during sampling at evening. The artificial exogenous electron donors diphenyl carbazide, NH2OH, and Mn2+ failed to restore the PS2 activity in both leaf types at midday. Thus CN enhanced inactivation on the acceptor side of PS2 in grapevine leaves. Quantification of the PS2 reaction centre protein D1 following midday exposure of leaves showed pronounced differences between C and CN leaves. The marked loss of PS2 activity in CN leaves noticed in midday samples was mainly due to the marked loss of D1 protein of the PS2 reaction centre.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Chl:

chlorophyll

DCBQ:

2,6-dichloro-p-benzoquinone

DCPIP:

2,6-dichlorophenol indophenol

DPC:

diphenyl carbazide

F0 :

minimal fluorescence

Fm :

maximum fluorescence

MV:

methyl viologen

PPFD:

photosynthetic photon flux density

PS:

photosystem

SDS-PAGE:

sodium dodecylsulphate-polyacrylamide gel electrophoresis

SiMo:

silicomolybdate

References

  • Andersson, B., Aro, E.-M.: Photodamage and D1 protein turnover in photosystem II. — In: Aro, E.-M., Andersson, B. (ed.): Advances in Photosynthesis and Photorespiration. Regulation of Photosynthesis. Pp. 377–393. Kluwer Academic Publishers, Dordrecht — Boston — London 2001.

    Google Scholar 

  • Aro, E.-M., Hundal, T., Carlberg, I., Andersson, B.: In vitro studies on light-induced inhibition of Photosystem II and D1-protein degradation at low temperatures. — Biochim. biophys. Acta 1019: 269–275, 1990.

    Google Scholar 

  • Aro, E.-M., Virgin, I., Andersson, B.: Photoinhibition of photosystem II. Inactivation, protein damage and turnover. — Biochim. biophys. Acta 1143: 113–134, 1993.

    PubMed  Google Scholar 

  • Berthold, D.A., Babcock, G.T., Yocum, C.F.: A highly resolved, oxygen-evolving photosystem II preparation from spinach thylakoid membranes. EPR and electron-transport properties. — FEBS Lett. 134: 231–234, 1981.

    Article  Google Scholar 

  • Buttrose, M.S.: Vegetative growth of grapevine varieties under controlled temperature and light intensity. — Vitis 8: 280–285, 1969.

    Google Scholar 

  • Carpentier, R.: Influence of high light intensity on photosynthesis: photoinhibition and energy dissipation. — In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 443–450. Marcel Dekker, New York — Basel — Hong Kong 1995.

    Google Scholar 

  • Chaumont, M., Morot-Gaudry, J.-F., Foyer, C.H.: Effects of photoinhibitory treatment on CO2 assimilation, the quantum yield of CO2 assimilation, D1 protein, ascorbate, glutathione and xanthophyll contents and the electron transport rate in vine leaves. — Plant Cell Environ. 18: 1358–1366, 1995.

    Google Scholar 

  • Chaumont, M., Osorio, M.L., Chaves, M.M., Vanacker, H., Morot-Gaudry, J.-F., Foyer, C.H.: The absence of photo-inhibition during mid-morning depression of photosynthesis in Vitis vinifera grown in semi-arid and temperate climates. — Plant Physiol. 150: 743–751, 1997.

    Google Scholar 

  • Critchley, C., Russell, A.W.: Photoinhibition of photosynthesis in vivo: The role of protein turnover in photosystem II. — Physiol. Plant. 92: 188–196, 1994.

    Article  Google Scholar 

  • Demmig-Adams, B., Adams, W.W., III: Photoprotection and other responses of plants to high light stress. — Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 599–626, 1992.

    Article  Google Scholar 

  • Demmig, B., Winter, K., Kruger, A., Czygen, F.-C.: Zeaxanthin and the heat dissipation of excess light energy in Nerium oleander exposed to a combination of high light and water stress. — Plant Physiol. 87: 17–24, 1988.

    Google Scholar 

  • Ebrahim, M.K.H., Vogg, G., Osman, M.E.H., Komor, E.: Photosynthetic performance and adaptation of sugarcane at suboptimal temperatures. — J. Plant Physiol. 153: 587–592, 1998.

    Google Scholar 

  • Eckert, H.-J., Geiken, B., Bernarding, J., Napiwotzki, A., Eichler, H.-J., Renger, G.: Two sites of photoinhibition of the electron transfer in oxygen evolving and Tris-treated PS II membrane fragments from spinach. — Photosynth. Res. 27: 97–108, 1991.

    Article  Google Scholar 

  • Flexas, J., Hendrickson, L., Chow, W.S.: Photoinactivation of photosystem II in high light-acclimated grapevines. — Aust. J. Plant Physiol. 28: 755–764, 2001.

    Google Scholar 

  • Groom, Q.J., Baker, N.R., Long, S.P.: Photoinhibition of holly (Ilex aquifolium) in the field duing the winter. — Physiol. Plant. 83: 585–590, 1991.

    Article  Google Scholar 

  • Hendrickson, L., Ball, M.C., Osmond, C.B., Furbank, R.T., Chow, W.S.: Assessment of photoprotection mechanisms of grapevine at low temperature. — Funct. Biol. 30: 631–642, 2003.

    Article  Google Scholar 

  • Hendrickson, L., Ball, M.C., Wood, J.T., Chow, W.S., Furbank, R.T.: Low temperature effects on photosynthesis and growth of grapevine. — Plant Cell Environ. 27: 795–809, 2004.

    Article  Google Scholar 

  • Hurry, V., Huner, N., Selstam, E., Gardestrom, P., Oquist, G.: Photosynthesis at low growth temperatures. — In: Raghavendra, A.S. (ed.): Photosynthesis. A Comprehensive Treatise. Pp. 238–249. Cambridge University Press, Cambridge 1998.

    Google Scholar 

  • Iacono, F., Bertamini, M., Mattivi, F.: Differential effects of canopy manipulation and shading. Note I. Composition of grape berries (Vitis vinifera L. cv. Cabernet Sauvignon). — Wein-Wiss. 49: 220–225, 1994.

    Google Scholar 

  • Krause, G.H.: Photoinhibition induced by low temperatures. — In: Baker, N.R., Bowyer, J.R. (ed.): Photoinhibition of Photosynthesis. From Molecular Mechanisms to the Field. Pp. 331–348. Bios Scientific Publ., Oxford 1994.

    Google Scholar 

  • Krause, G.H., Weis, E.: Chlorophyll fluorescence and photosynthesis: The basics. — Annu. Rev. Plant. Physiol. Plant mol. Biol. 42: 313–349, 1991.

    Article  Google Scholar 

  • Kyle, D.J., Ohad, I.: The mechanism of photoinhibition in higher plants and green algae. — In: Staehelin, L.A., Arntzen, C.J. (ed.): Photosynthesis III. Pp. 468–475. Springer-Verlag, Berlin — Heidelberg — New York — Tokyo 1986.

    Google Scholar 

  • Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. — Nature 227: 680–685, 1970.

    Article  PubMed  Google Scholar 

  • Lee, H.-Y., Chow, W.S., Hong, Y.-N.: Photoinactivation of photosystem II in leaves of Capsicum annuum. — Physiol. Plant. 105: 377–384, 1999.

    Article  Google Scholar 

  • Long, S.P., East, T.M., Baker, N.R.: Chilling damage to photosynthesis in young Zea mays. I. Effects of light and temperature variation on photosynthetic CO2 assimilation. — J. exp. Bot. 34: 177–188, 1983.

    Google Scholar 

  • Lutz, C., Steiger, A., Godde, D.: Influence of air pollutants and nutrient deficiency on D-1 protein content and photosynthesis in young spruce trees. — Physiol. Plant. 85: 611–617, 1992.

    Article  Google Scholar 

  • Morot-Gaudry, J.-F., Farineau, J., Lelandais, M., Moutot, F., Powles, S., Cornic, G.: Photoinhibition in leaves of Phaseolus vulgaris: metabolic approach. — Physiol. veg. 24: 541–549, 1986.

    Google Scholar 

  • Murata, N., Nishiyama, Y.: Molecular mechanisms of the low-temperature tolerance of the photosynthetic machinery. — In: Satoh, K., Murata, N. (ed.): Stress Responses of Photosynthetic Organisms. Pp. 93–112. Elsevier, Amsterdam — Lausanne — New York — Oxford — Shannon — Singapore — Tokyo 1998.

    Google Scholar 

  • Nedunchezhian, N., Morales, F., Abadia, A., Abadia, J.: Decline in photosynthetic electron transport activity and changes in thylakoid protein pattern in field grown iron deficient peach (Prunus persica L.). — Plant Sci. 129: 29–38, 1997.

    Article  Google Scholar 

  • Ohad, I., Kyle, D.J., Hirschberg, J.: Light-dependent degradation of the QB-protein in isolated pea thylakoids. — EMBO J. 4: 1655–1659, 1985.

    Google Scholar 

  • Ortiz-Lopez, A., Nie, G.Y., Ort, D.R., Baker, N.R.: The involvement of the photoinhibition of photosystem II and impaired membrane energization in the reduced quantum yield of carbon assimilation in chilled maize. — Planta 181: 78–84, 1990.

    Article  Google Scholar 

  • Osmond, C.B.: What is photoinhibition? Some insights from comparison of shade and sun plants. — In: Baker, N.R., Bowyer, J.R. (ed.): Photoinhibition: Molecular Mechanism to the Field. Pp. 1–24. Bioscience, Oxford 1994.

    Google Scholar 

  • Prasil, O., Adir, N., Ohad, I.: Dynamics of photosystem II: mechanism of photoinhibition and recovery processes. — In: Barber, J. (ed.): The Photosystems: Structure, Function and Molecular Biology. Pp. 250–348. Elsevier, Amsterdam — London — New York — Tokyo 1992.

    Google Scholar 

  • Salonen, M., Aro, E.-M., Rintamaki, E.: Reversible phosphorylation and turnover of the D1 protein under various redox states of Photosystem II induced by low temperature photoinhibition. — Photosynth. Res. 58: 143–151, 1998.

    Article  Google Scholar 

  • Schiefthaler, U., Russell, A.W., Bolhar-Nordenkampf, H.R., Critchley, C.: Photoregulation and photodamage in Schefflera arboricola leaves adapted to different light environments. — Aust. J. Plant Physiol. 26: 485–494, 1999.

    Google Scholar 

  • Setlik, I., Allakhverdiev, S.I., Nedbal, L., Setlikova, E., Klimov, V.V.: Three types of photosystem II photoinactivation. 1. Damaging processes on the acceptor side. — Photosynth. Res. 23: 39–48, 1990.

    Article  Google Scholar 

  • Smith, B.M., Morrissey, P.J., Guenther, J.E., Nemson, J.A., Harrison, M.A., Allen, J.F., Melis, A.: Response of the photosynthetic apparatus in Dunaliella salina (green algae) to irradiance stress. — Plant Physiol. 93: 1433–1440, 1990.

    Google Scholar 

  • Sonoike, K.: The different roles of chilling temperatures in the photoinhibition of PSI and PSII. — J. Photochem. Photobiol. B 48: 136–141, 1999.

    Article  Google Scholar 

  • Tjus, S.E., Moller, B.L., Scheller, H.V.: Photosystem I is an early target of photoinhibition in barley illuminated at chilling temperatures. — Plant Physiol. 116: 755–764, 1998.

    Article  PubMed  Google Scholar 

  • Yamane, Y., Kashino, Y., Koike, H., Satoh, K.: Increases in the fluorescence Fo level of reversible inhibition of photosystem II reaction centre by high-temperature treatments in higher plants. — Photosynth. Res. 52: 57–64, 1997.

    Article  Google Scholar 

  • Wydrzynski, T., Govindjee: A new site of bicarbonate effect in photosystem II of photosynthesis: Evidence from chlorophyll fluorescence transients in spinach chloroplasts. — Biochim. biophys. Acta 387: 403–408, 1975.

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. N. Nedunchezhian

    Present address: Govt. HSS. Vellimedupettai, 604 207, India

Authors and Affiliations

  1. Istituto Agrario di San Michele all'Adige, 38010, San Michele all'Adige, Italy

    M. Bertamini, M. Rubinigg, R. Zorer & N. Nedunchezhian

  2. Centre for Biodiversity and Forest Studies, School of Energy Sciences, Madurai Kamaraj University, Madurai, 625 021, India

    K. Muthuchelian & N. Nedunchezhian

Authors
  1. M. Bertamini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Muthuchelian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Rubinigg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Zorer
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. Nedunchezhian
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. Nedunchezhian.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bertamini, M., Muthuchelian, K., Rubinigg, M. et al. Photoinhibition of photosynthesis in leaves of grapevine (Vitis vinifera L. cv. Riesling). Effect of chilling nights. Photosynthetica 43, 551–557 (2005). https://doi.org/10.1007/s11099-005-0087-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11099-005-0087-x

Additional keywords

Search

Navigation