Skip to main content
SpringerLink
Log in

The effect of additional red irradiation on the photosynthetic apparatus of Pisum sativum

  • Published:
Photosynthetica

Abstract

Pisum sativum (L.) plants were grown under “white” luminescent lamps, W [45 µ mol(quantum) m−2 s−1] or under the same irradiation supplemented with narrow spectrum red light-emitting diodes (LEDs), RE [λmax = 660 nm, Δλ = 20 nm, 40 µmol(quantum) m−2 s−1]. Significant differences in the chlorophyll (Chl) a fluorescence parameters, degree of State 1–State 2 transition, and the pigment-protein contents were found in plants grown under differing spectral composition. Addition of red LEDs to the “white light” resulted in higher effective quantum yield of photosystem 2 (PS2), i.e. F′v/F′m, linear electron transport (ϕPS2), photochemical quenching (qP), and lower non-photochemical quenching (qN as well as NPQ). The RE plants were characterised by higher degree State 1–State 2 transition, i.e. they were more effective in radiant energy utilisation. Judging from the data of “green” electrophoresis of Chl containing pigment-protein complexes of plants grown under various irradiation qualities, the percentage of Chl in photosystem 2 (PS2) reaction centre complexes in RE plants was higher and there was no difference in the total Chl bound with Chl-proteins of light-harvesting complexes (LHC2). Because the ratio between oligomeric and monomeric LHC2 forms was higher in RE plants, we suggest higher LHC2 stability in these ones.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Chl:

chlorophyll

F0 :

ground value of fluorescence

Fv, Fm :

variable and maximum fluorescence in the dark-adapted state, respectively

Fv/Fm :

maximum quantum efficiency of PS2 photochemistry

F′v/F′m :

maximum quantum efficiency of PS2 under given irradiance

LEDs:

light-emitting diodes

LHC2:

light-harvesting complexes of PS2

PS:

photosystem

QA:

primary quinone of PS2

qP, qN :

photochemical and non-photochemical quenching of chlorophyll fluorescence, respectively

RE:

red-enriched “white growth light”

W:

“white growth light”

ϕPS2 :

quantum efficiency of linear electron transport through PS2

References

  • Allen, J.F.: Thylakoid protein phosphorylation, state 1–state 2 transitions, and photosystem stoichiometry adjustment: Redox control at multiple levels of gene expression. — Physiol. Plant. 93: 196–205, 1995.

    Article  Google Scholar 

  • Anderson, J.M.: P-700 content and polypeptide profile of chlorophyll-protein complexes of spinach and barley thylakoids. — Biochim. biophys. Acta 591: 113–126, 1980.

    PubMed  Google Scholar 

  • Anderson, J.M., Osmond, C.B.: Shade-sun responses: compromises between acclimation and photoinhibition. — In: Kyle, D.J., Osmond, C.B., Arntzen, C.J. (ed.): Photoinhibition. Pp. 1–38. Elsevier, Amsterdam — New York — Oxford 1987.

    Google Scholar 

  • Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. — Plant Physiol. 24: 1–15, 1949.

    Google Scholar 

  • Astaphurova, T.P., Verchoturova, G.C., Zaycheva, T.A., Rakitin, A.V., Victorova, I.A., Aminov, P.I.: [The effect of different spectral region PAR ratios on the photosynthetic metabolism of the cucumber plants.] — Vest. bashkirsk. Univ. 2: 29–32, 2001. [In Russ.]

    Google Scholar 

  • Bassi, R., Dainese, P.: The role of light harvesting complex II and of the minor chlorophyll a/b proteins in the organization of the photosystem II antenna system. — In: Baltscheffsky, M. (ed.): Current Researches in Photosynthesis. Vol. II. Pp. 209–216. Kluwer Academic Publ., Dordercht — Boston — London 1990.

    Google Scholar 

  • Bilger, W., Bjorkman, O.: Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in Hedera canariensis. — Photosynth. Res. 25: 173–185, 1990.

    Article  Google Scholar 

  • Carlberg, I., Andersson, B.: Changed lateral migration of phospho-LHCII in the thylakoid membrane upon acclimation of spinach to low temperatures. — FEBS Lett. 333: 10–14, 1993.

    Article  PubMed  Google Scholar 

  • Chow, W.S., Melis, A., Anderson, J.M.: Adjustments of photosystem stoichiometry in chloroplasts improve the quantum efficiency of photosynthesis. — Proc. nat. Acad. Sci. USA. 87: 7502–7506, 1990.

    PubMed  Google Scholar 

  • Franklin, K.A., Whitelam, G.C.: Light signals, phytochromes and cross-talk with other environmental cues. — J. exp. Bot. 55: 271–276, 2003.

    Article  PubMed  Google Scholar 

  • Garab, G., Cseh, Z., Kovacs, L., Rajagopal, S., Varkonyi, Z., Wentworth, M., Mustardy, L., Der, A., Ruban, A., Papp, E., Holzenburg, A., Horton, P.: Light-induced trimer to monomer transition in the main light-harvesting antenna complex of plants: Thermo-optic mechanism. — Biochemistry 41: 15121–15129, 2002.

    Article  PubMed  Google Scholar 

  • Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. — Biochim. biophys. Acta 990: 87–92, 1989.

    Google Scholar 

  • Goins, G.D., Yorio, N.C., Sanwoo, M.M., Brown, C.S.: Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LEDs) with and without supplemental blue lighting. — J. exp. Bot. 48: 1407–1413, 1997.

    PubMed  Google Scholar 

  • Hodges, M., Barber, J.: Photosynthetic adaptation of pea plants grown at different light intensities: State 1–State 2 transitions and associated chlorophyll fluorescence changes. — Planta 157: 166–173, 1983.

    Article  Google Scholar 

  • Horton, P., Ruban, A.V., Walters, R.G.: Regulation of light harvesting in green plants. — Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 655–684, 1996.

    Article  PubMed  Google Scholar 

  • Iordanov, I.T., Goltsev, V.N.: Regulation of photosynthesis at the level of phosphorylation of the light-harvesting complex and photophosphorylation. — Photosynthetica 21: 236–250, 1987.

    Google Scholar 

  • Leong T.Y., Andersson, J.M.: Adaptation of the thylakoid membranes of pea chloroplast to light intensities. I. Study on the distribution of chlorophyll-protein complexes. — Photosynth. Res. 5: 105–115, 1984.

    Article  Google Scholar 

  • Lichtenthaler, H.K., Burkart, S.: Photosynthesis and high light stress. — Bulg. J. Plant Physiol. 25: 3–16, 1999.

    Google Scholar 

  • Lichtenthaler, H.K., Buschmann, C., Doll, M., Fietz, H.-J., Bach, T., Kozel, U., Meier, D., Rahmsdorf, U.: Photosynthetic activity, chloroplast ultrastructure, and leaf characteristic of high-light and low-light plants and of sun and shade leaves. — Photosynth. Res. 2: 115–141, 1981.

    Article  Google Scholar 

  • Lichtenthaler, H.K., Kuhn, G., Prenzel, U.: Adaptation of chloroplast-ultrastructure and of chlorophyll-protein levels to high-light and low-light growth conditions. — Z. Naturforsch. 37c: 464–475, 1982.

    Google Scholar 

  • Lopez-Juez, E., Buurmeijer, W.F., Heeringa, G.H., Kendrick, R.E., Wesseliuss, J.C.: Response of light-grown wild-type and long hypocotyl mutant cucumber plants to end of day far-red light. — Photochem. Photobiol. 52: 143–149, 1990.

    Google Scholar 

  • Maxwell, K., Johnson, G.N.: Chlorophyll fluorescence — a practical guide. — J. exp. Bot. 51: 659–668, 2000.

    Article  PubMed  Google Scholar 

  • Peter, G.F., Thornber, J.P.: Biochemical composition and organization of higher plant photosystem II light-harvesting-proteins. — J. biol. Chem. 266: 16745–16754, 1991.

    PubMed  Google Scholar 

  • Pfannschmidt, T., Schutze, K., Brost, M., Oelmuller, R.A.: A novel mechanism of nuclear photosynthesis gene regulation by redox signals from the chloroplast during photosystem stoichiometry adjustment. — J. biol. Chem. 276: 36125–36130, 2001.

    Article  PubMed  Google Scholar 

  • Protasova, N.N., Uells, D.M., Dobrovol'skiy, M.V., Tsoglin, L.N.: [Spectral characteristics of light sources and features of plants growth under artificial illumination.] — Fiziol. Rast. 37: 386–396, 1990. [In Russ.]

    Google Scholar 

  • Schreiber, U., Schliwa, U., Bilger, W.: Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. — Photosynth. Res. 10: 51–62, 1986.

    Article  Google Scholar 

  • Smith, H., Samson, G., Fork, D.C.: Photosynthetic acclimation to shade-probing the role of phytochromes using photomorphogenic mutants of tomato. — Plant Cell Environ. 16, 929–937, 1993.

    Google Scholar 

  • Stroch, M., Spunda, V., Kurasova, I.: Non-radiative dissipation of absorbed excitation energy within photosynthetic apparatus of higher plants. — Photosynthetica 42: 323–337, 2004.

    Article  Google Scholar 

  • Topchiy, N.M., Syvash, O.O., Fomishina, R.M.: [The influence of different light intensity and spectral composition on pigment apparatus and functional characteristics of pea chloroplasts (Pisum sativum L.).] — Ukr. bot. Zh. 61: 93–100, 2004. [In Ukrain.]

    Google Scholar 

  • Walters, R.G., Horton, P.: Acclimation of Arabidopsis thaliana to the light environment: changes in composition of the photosynthetic apparatus. — Planta 195: 248–256, 1994.

    Article  Google Scholar 

  • Walters, R.G., Horton, P.: Acclimation of Arabidopsis thaliana to the light environment: changes in photosynthetic function. — Planta 197: 306–312, 1995.

    PubMed  Google Scholar 

  • Wentworth, M., Ruban, A., Horton, P.: Kinetic analysis of non-photochemical quenching of chlorophyll fluorescence. 2. Isolated light-harvesting complexes. — Biochemistry 40: 9902–9908, 2001.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, 2 Tereschenkivska Str., 01004, Kyiv, Ukraine

    N. M. Topchiy, S. K. Sytnik, O. O. Syvash & O. K. Zolotareva

Authors
  1. N. M. Topchiy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. K. Sytnik
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. O. Syvash
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. O. K. Zolotareva
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Topchiy, N.M., Sytnik, S.K., Syvash, O.O. et al. The effect of additional red irradiation on the photosynthetic apparatus of Pisum sativum . Photosynthetica 43, 451–456 (2005). https://doi.org/10.1007/s11099-005-0072-4

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11099-005-0072-4

Additional key words

Search

Navigation