Abstract
Life and research results of Pavel Siffel, a talented but untimely deceased Czech scientist in photosynthesis, are reviewed. He studied biophysics and physiology of chlorophyll, its complexes with proteins, their absorption and fluorescence spectra, activities in mutants and transformants, dealt with chlorophyll biosynthesis and protochlorophyllide photoreduction, pigments in plants grown at CO2 deficiency and under simulated acid rain, with changes accompanying leaf and plant development, photobleaching, etc. He participated in construction of specialised spectrofluorometers, finally he built the kinetic spectrophotometer SpeKin.
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Bibliography of Pavel Siffel
Bertrand, M., Schoefs, B., Siffel, P., Rohacek, K., Molnar, I.: Cadmium inhibits epoxidation of diatoxanthin to diadinoxanthin in the xantophyll cycle of the marine diatom Phaeodactylum tricornutum. — FEBS Lett. 508: 153–156, 2001.
Koblizek, M., Ciscato, M., Komenda, J., Kopecky, J., Siffel, P., Masojidek, J.: Photoadaptation in the green alga Spongiochloris sp. A three-fluorometer study. — Photosynthetica 37: 307–323, 1999.
Lang, M., Siffel, P., Braunova, Z., Lichtenthaler, H.K.: Investigations of the blue-green fluorescence emission of plant leaves. — Bot. Acta 105: 435–440, 1992.
Lebedev, N.N., Pakshina, E.V., Shiffel, P., Krasnovskii, A.A.: [Spectral effects of the interaction between hydrogen ions and chloroplast membrane.] — Biofizika 30: 1000–1003, 1985a. [In Russ.]
Lebedev, N.N., Pakshina, E.V., Shiffel, P., Krasnovskii, A.A.: [Formation of various spectral forms of pheophytin in chloroplasts in acid media.] — Biokhimiya 51: 33–38, 1986a. [In Russ.]
Lebedev, N.N., Shiffel, P., Krasnovskii, A.A.: [Spectral characteristics of fluorescence of protochlorophyllide and chlorophyll b in green leaves and isolated chloroplasts.] — Biofizika 30: 44–49, 1985b. [In Russ.]
Lebedev, N.N., Siffel, P., Krasnovskii, A.A.: Detection of protochlorophyllide forms in irradiated green leaves and chloroplasts by difference fluorescence spectroscopy at 77 K. — Photosynthetica 19: 183–187, 1985c.
Lebedev, N.N., Siffel, P., Krasnovsky, A.A.: Free chlorophylls in green plant cells. — In: Murata, N. (ed.): Research in Photosynthesis. Vol. III. Pp. 127–130. Kluwer Academic Publ., Dordrecht-Boston-London 1992a.
Lebedev, N.N., Siffel, P., Nozdrina, V.N., Filipovich, I.I.: Free chlorophylls in green plants. — Photosynth. Res. 34: 116, 1992b.
Lebedev, N.N., Siffel, P., Pakshina, E.V., Krasnovskii, A.A.: The effect of acidification on absorption and fluorescence spectra of French bean chloroplasts and the kinetics of pheophytin formation. — Photosynthetica 20: 124–130, 1986b.
Lebedev, N.N., Siffel, P., Sestak, Z.: Composition of chlorophyll-protein complexes and fluorescence spectrum changes during ontogeny of primary leaves of Phaseolus vulgaris L. — Photosynthetica 20: 146–152, 1986c.
Litvin, R., Bina, D., Siffel, P., Vacha, F.: Conformational changes and their role in non-radiative energy dissipation in photosystem II reaction centres. — Photochem. Photobiol. Sci., in press, 2005.
Rohacek, K., Siffel, P.: Temperature and light dependences of chlorophyll fluorescence in vivo for Norway spruce seedlings. — In: Mathis, P. (ed.): Photosynthesis: from Light to Biosphere. Vol. V. Pp. 937–940. Kluwer Academic Publ., Dordrecht-Boston-London 1995.
Santrucek, J., Siffel, P., Lang, M., Lichtenthaler, H.K., Schindler, C., Synkova, H., Konecna, V., Szabo, K.: Photosynthetic activity and chlorophyll fluorescence parameters in aurea and green forms of Nicotiana tabacum. — Photosynthetica 27: 529–543, 1992.
Santrucek, J., Siffel, P., Polisenska, M., Peskova, H.: Species-specific response of photosynthetic apparatus of tobacco and potato to low CO2 concentrations. — Photosynthetica 25: 203–209, 1991.
Sestak, Z., Siffel, P.: Changes in chloroplast fluorescence during leaf development. — In: Lichtenthaler, H.K. (ed.): Applications of Chlorophyll Fluorescence. Pp. 85–91. Kluwer Academic Publ., Dordrecht-Boston-London 1988.
Sestak, Z., Siffel, P.: Leaf-age related differences in chlorophyll fluorescence. — Photosynthetica 33: 347–369, 1997.
Shiffel, P., Lebedev, N.N.: [Chlorophyll water-soluble complex in green leaves and isolated chloroplasts.]-Biofizika 33: 804–808, 1988. [In Russ.]
Siffel, P., Braunova, Z.: Release and aggregation of the light-harvesting complex in intact leaves subjected to strong CO2 deficit. — Photosynth. Res. 61: 217–226, 1999.
Siffel, P., Braunova, Z., Sindelkova, E., Cudlin, P.: The effect of simulated acid rain on chlorophyll fluorescence spectra of spruce seedlings (Picea abies L. Karst.). — J. Plant Physiol. 148: 271–275, 1996.
Siffel, P., Durchan, M., Kveton, J., Ondrej, M., Sestak, Z.: Photosynthesis in regenerants of tobacco transformed by plasmids of Agrobacterium. 2. Fluorescence emission spectra and age induced changes in slow fluorescence induction. — Photosynthetica 22: 214–220, 1988.
Siffel, P., Hunalova, I., Rohacek, K.: Light-induced quenching of chlorophyll fluorescence at 77 K in thylakoids and whole leaves. — In: Garab, G. (ed.): Photosynthesis: Mechanisms and Effects. Vol. I. Pp. 393–396. Kluwer Academic Publ., Dordrecht-Boston-London 1998.
Siffel, P., Hunalova, I., Rohacek, K.: Light-induced quenching of chlorophyll fluorescence at 77 K in leaves, chloroplasts and Photosystem II particles. — Photosynth. Res. 65: 219–229, 2000.
Siffel, P., Kutik, J., Lebedev, N.N.: Spectroscopically analysed degradation of chlorophyll-protein complexes and chloroplast ultrastructure during yellowing of leaves. — Photosynthetica 25: 395–407, 1991.
Siffel, P., Lebedev, N.N., Krasnovskii, A.A.: Detection of short-wavelength chlorophyll a emission in green leaves. — Photosynthetica 21: 23–28, 1987.
Siffel, P., Lebedev, N.N., Sestak, Z.: Changes in chloroplast absorption and fluorescence spectra during ontogeny of primary bean leaves. — Photosynthetica 19: 127–137, 1985.
Siffel, P., Lebedev, N.N., Sestak, Z.: Changes in shape of chloroplast spectra during ontogeny of primary leaves of French bean correlate with rate of chlorophyll accumulation. — In: Akoyunoglou, G., Senger, H. (ed.): Regulation of Chloroplast Differentiation. Pp. 691–696. A.R. Liss, New York 1986.
Siffel, P., Santrucek, J.: Diurnal course of photosynthetic activity of winter-adapted Scots pine at subzero temperatures. — Photosynthetica 43: 395–402, 2005.
Siffel, P., Santrucek, J., Lang, M., Braunova, Z., Simkova, M., Synkova, H., Lichtenthaler, H.K.: Age dependence of photosynthetic activity, chlorophyll fluorescence parameters and chloroplast ultrastructure in aurea and green forms of Nicotiana tabacum Su/su mutant. — Photosynthetica 29: 81–94, 1993.
Siffel, P., Sestak, Z.: Low temperature fluorescence spectra of chloroplasts: methodical aspects and possible applications. — In: Lichtenthaler, H.K. (ed.): Application of Chlorophyll Fluorescence. Pp. 55–61. Kluwer Academic Publ., Dordrecht-Boston-London 1988.
Siffel, P., Sestak, Z.: Seasonal changes in the development of pigment apparatus of primary leaves of French bean.-Photosynthetica 23: 227–229, 1989.
Siffel, P., Sindelkova, E., Durchan, M., Zajicova, M.: Photosynthetic characteristics of Solanum tuberosum L. plants transformed by Agrobacterium strains. 1. Pigment apparatus. — Photosynthetica 27: 441–447, 1992.
Siffel, P., Vacha, F.: LHC aggregation in intact leaves of tobacco plants stressed by CO2 starvation. — In: Mathis, P. (ed.): Photosynthesis: from Light to Biosphere. Vol. I. Pp. 111–114. Kluwer Academic Publ., Dordrecht-Boston-London 1995.
Siffel, P., Vacha, F.: Aggregation of the light-harvesting complex in intact leaves of tobacco plants stressed by CO2 deficit. — Photochem. Photobiol. 67: 304–311, 1998.
Siffel, P., Vacha, F.: Pheophytin reduction and fluorescence quenching in reaction centres of Photosystem II.-PS 2001 Proceedings. 12th International Congress on Photosynthesis. S22-034. Pp. 1–4, CSIRO, Collingwood 2001.
Siffel, P., Vavrinec, E.: Excitation energy transfer between chlorophylls b and a in polystyrene foils.-Photosynthetica 14: 477–481, 1980.
Stys, D., Siffel, P., Hunalova, I., Nebesarova, J.: The relation between changes in non-photochemical quenching, low temperature fluorescence emission, and membrane ultrastructure upon binding of polyionic compounds and fragments of light-harvesting complex 2. — Photosynthetica 37: 325–334, 1999.
Synkova, H., Pechova, R., Polanska, L., Husak, M., Siffel, P., Vacha, F., Malbeck, J., Machackova, I., Nebesarova, J.: Three-dimensional reconstructi on of anomalous chloroplasts in transgenic ipt tobacco.-Planta, accepted, 2005a.
Synkova, H., Schnablova, R., Husak, M., Siffel, P., Bumba, L., Hunalova, I., Vacha, F.: Isolation and characterization of paracrystalline structures from transgenic Pssu-ipt tobacco. — Photosynthetica 43: accepted, 2005b.
Vacha, F., Durchan, M., Siffel, P.: Excitonic interactions in the reaction centre of photosystem II studied by circular dichroism. — PS 2001 Proceedings. 12th International Congress on Photosynthesis. S22-004. Pp. 1–6. CSIRO, Collingwood 2001.
Vacha, F., Durchan, M., Siffel, P.: Excitonic interactions in the reaction centre of photosystem II studied by using circular dichroism. — Biochim. biophys. Acta 1554: 147–152, 2002.
Vacha, F., Kuty, M., Durchan, M., Siffel, P., Psencik, J.: Localisation of accumulated chlorophyll cation in reaction centre of photosystem II. — Mater. Struct. 10: 21–24, 2003.
Vacha, F., Psencik, P., Kuty, M., Durchan, M., Siffel, P.: Evidence for localisation of accumulated chlorophyll cation on the D1-accessory chlorophyll in reaction centre of photosystem II. — Photosynth. Res. 83: 279–302, 2005.
Zehetner, A., Scheer, H., Siffel, P., Vacha, F.: Photosystem II reaction center with altered pigment-composition: reconstitution of a complex containing five chlorophyll a per two pheophytin a with modified chlorophylls.-Biochim. biophys. Acta 1556: 21–28, 2002.
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Kveton, J., Durchan, M., Rohacek, K. et al. Pavel Siffel (1954–2003) or Life full of chlorophyll. Photosynthetica 43, 323–328 (2005). https://doi.org/10.1007/s11099-005-0055-5
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DOI: https://doi.org/10.1007/s11099-005-0055-5