Skip to main content
Log in

Pavel Siffel (1954–2003) or Life full of chlorophyll

  • Review
  • Published:
Photosynthetica

Abstract

Life and research results of Pavel Siffel, a talented but untimely deceased Czech scientist in photosynthesis, are reviewed. He studied biophysics and physiology of chlorophyll, its complexes with proteins, their absorption and fluorescence spectra, activities in mutants and transformants, dealt with chlorophyll biosynthesis and protochlorophyllide photoreduction, pigments in plants grown at CO2 deficiency and under simulated acid rain, with changes accompanying leaf and plant development, photobleaching, etc. He participated in construction of specialised spectrofluorometers, finally he built the kinetic spectrophotometer SpeKin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Bibliography of Pavel Siffel

  • Bertrand, M., Schoefs, B., Siffel, P., Rohacek, K., Molnar, I.: Cadmium inhibits epoxidation of diatoxanthin to diadinoxanthin in the xantophyll cycle of the marine diatom Phaeodactylum tricornutum. — FEBS Lett. 508: 153–156, 2001.

    Article  PubMed  Google Scholar 

  • Koblizek, M., Ciscato, M., Komenda, J., Kopecky, J., Siffel, P., Masojidek, J.: Photoadaptation in the green alga Spongiochloris sp. A three-fluorometer study. — Photosynthetica 37: 307–323, 1999.

    Article  Google Scholar 

  • Lang, M., Siffel, P., Braunova, Z., Lichtenthaler, H.K.: Investigations of the blue-green fluorescence emission of plant leaves. — Bot. Acta 105: 435–440, 1992.

    Google Scholar 

  • Lebedev, N.N., Pakshina, E.V., Shiffel, P., Krasnovskii, A.A.: [Spectral effects of the interaction between hydrogen ions and chloroplast membrane.] — Biofizika 30: 1000–1003, 1985a. [In Russ.]

    Google Scholar 

  • Lebedev, N.N., Pakshina, E.V., Shiffel, P., Krasnovskii, A.A.: [Formation of various spectral forms of pheophytin in chloroplasts in acid media.] — Biokhimiya 51: 33–38, 1986a. [In Russ.]

    Google Scholar 

  • Lebedev, N.N., Shiffel, P., Krasnovskii, A.A.: [Spectral characteristics of fluorescence of protochlorophyllide and chlorophyll b in green leaves and isolated chloroplasts.] — Biofizika 30: 44–49, 1985b. [In Russ.]

    Google Scholar 

  • Lebedev, N.N., Siffel, P., Krasnovskii, A.A.: Detection of protochlorophyllide forms in irradiated green leaves and chloroplasts by difference fluorescence spectroscopy at 77 K. — Photosynthetica 19: 183–187, 1985c.

    Google Scholar 

  • Lebedev, N.N., Siffel, P., Krasnovsky, A.A.: Free chlorophylls in green plant cells. — In: Murata, N. (ed.): Research in Photosynthesis. Vol. III. Pp. 127–130. Kluwer Academic Publ., Dordrecht-Boston-London 1992a.

    Google Scholar 

  • Lebedev, N.N., Siffel, P., Nozdrina, V.N., Filipovich, I.I.: Free chlorophylls in green plants. — Photosynth. Res. 34: 116, 1992b.

    Google Scholar 

  • Lebedev, N.N., Siffel, P., Pakshina, E.V., Krasnovskii, A.A.: The effect of acidification on absorption and fluorescence spectra of French bean chloroplasts and the kinetics of pheophytin formation. — Photosynthetica 20: 124–130, 1986b.

    Google Scholar 

  • Lebedev, N.N., Siffel, P., Sestak, Z.: Composition of chlorophyll-protein complexes and fluorescence spectrum changes during ontogeny of primary leaves of Phaseolus vulgaris L. — Photosynthetica 20: 146–152, 1986c.

    Google Scholar 

  • Litvin, R., Bina, D., Siffel, P., Vacha, F.: Conformational changes and their role in non-radiative energy dissipation in photosystem II reaction centres. — Photochem. Photobiol. Sci., in press, 2005.

  • Rohacek, K., Siffel, P.: Temperature and light dependences of chlorophyll fluorescence in vivo for Norway spruce seedlings. — In: Mathis, P. (ed.): Photosynthesis: from Light to Biosphere. Vol. V. Pp. 937–940. Kluwer Academic Publ., Dordrecht-Boston-London 1995.

    Google Scholar 

  • Santrucek, J., Siffel, P., Lang, M., Lichtenthaler, H.K., Schindler, C., Synkova, H., Konecna, V., Szabo, K.: Photosynthetic activity and chlorophyll fluorescence parameters in aurea and green forms of Nicotiana tabacum. — Photosynthetica 27: 529–543, 1992.

    Google Scholar 

  • Santrucek, J., Siffel, P., Polisenska, M., Peskova, H.: Species-specific response of photosynthetic apparatus of tobacco and potato to low CO2 concentrations. — Photosynthetica 25: 203–209, 1991.

    Google Scholar 

  • Sestak, Z., Siffel, P.: Changes in chloroplast fluorescence during leaf development. — In: Lichtenthaler, H.K. (ed.): Applications of Chlorophyll Fluorescence. Pp. 85–91. Kluwer Academic Publ., Dordrecht-Boston-London 1988.

    Google Scholar 

  • Sestak, Z., Siffel, P.: Leaf-age related differences in chlorophyll fluorescence. — Photosynthetica 33: 347–369, 1997.

    Google Scholar 

  • Shiffel, P., Lebedev, N.N.: [Chlorophyll water-soluble complex in green leaves and isolated chloroplasts.]-Biofizika 33: 804–808, 1988. [In Russ.]

    Google Scholar 

  • Siffel, P., Braunova, Z.: Release and aggregation of the light-harvesting complex in intact leaves subjected to strong CO2 deficit. — Photosynth. Res. 61: 217–226, 1999.

    Article  Google Scholar 

  • Siffel, P., Braunova, Z., Sindelkova, E., Cudlin, P.: The effect of simulated acid rain on chlorophyll fluorescence spectra of spruce seedlings (Picea abies L. Karst.). — J. Plant Physiol. 148: 271–275, 1996.

    Google Scholar 

  • Siffel, P., Durchan, M., Kveton, J., Ondrej, M., Sestak, Z.: Photosynthesis in regenerants of tobacco transformed by plasmids of Agrobacterium. 2. Fluorescence emission spectra and age induced changes in slow fluorescence induction. — Photosynthetica 22: 214–220, 1988.

    Google Scholar 

  • Siffel, P., Hunalova, I., Rohacek, K.: Light-induced quenching of chlorophyll fluorescence at 77 K in thylakoids and whole leaves. — In: Garab, G. (ed.): Photosynthesis: Mechanisms and Effects. Vol. I. Pp. 393–396. Kluwer Academic Publ., Dordrecht-Boston-London 1998.

    Google Scholar 

  • Siffel, P., Hunalova, I., Rohacek, K.: Light-induced quenching of chlorophyll fluorescence at 77 K in leaves, chloroplasts and Photosystem II particles. — Photosynth. Res. 65: 219–229, 2000.

    Article  Google Scholar 

  • Siffel, P., Kutik, J., Lebedev, N.N.: Spectroscopically analysed degradation of chlorophyll-protein complexes and chloroplast ultrastructure during yellowing of leaves. — Photosynthetica 25: 395–407, 1991.

    Google Scholar 

  • Siffel, P., Lebedev, N.N., Krasnovskii, A.A.: Detection of short-wavelength chlorophyll a emission in green leaves. — Photosynthetica 21: 23–28, 1987.

    Google Scholar 

  • Siffel, P., Lebedev, N.N., Sestak, Z.: Changes in chloroplast absorption and fluorescence spectra during ontogeny of primary bean leaves. — Photosynthetica 19: 127–137, 1985.

    Google Scholar 

  • Siffel, P., Lebedev, N.N., Sestak, Z.: Changes in shape of chloroplast spectra during ontogeny of primary leaves of French bean correlate with rate of chlorophyll accumulation. — In: Akoyunoglou, G., Senger, H. (ed.): Regulation of Chloroplast Differentiation. Pp. 691–696. A.R. Liss, New York 1986.

    Google Scholar 

  • Siffel, P., Santrucek, J.: Diurnal course of photosynthetic activity of winter-adapted Scots pine at subzero temperatures. — Photosynthetica 43: 395–402, 2005.

    Google Scholar 

  • Siffel, P., Santrucek, J., Lang, M., Braunova, Z., Simkova, M., Synkova, H., Lichtenthaler, H.K.: Age dependence of photosynthetic activity, chlorophyll fluorescence parameters and chloroplast ultrastructure in aurea and green forms of Nicotiana tabacum Su/su mutant. — Photosynthetica 29: 81–94, 1993.

    Google Scholar 

  • Siffel, P., Sestak, Z.: Low temperature fluorescence spectra of chloroplasts: methodical aspects and possible applications. — In: Lichtenthaler, H.K. (ed.): Application of Chlorophyll Fluorescence. Pp. 55–61. Kluwer Academic Publ., Dordrecht-Boston-London 1988.

    Google Scholar 

  • Siffel, P., Sestak, Z.: Seasonal changes in the development of pigment apparatus of primary leaves of French bean.-Photosynthetica 23: 227–229, 1989.

    Google Scholar 

  • Siffel, P., Sindelkova, E., Durchan, M., Zajicova, M.: Photosynthetic characteristics of Solanum tuberosum L. plants transformed by Agrobacterium strains. 1. Pigment apparatus. — Photosynthetica 27: 441–447, 1992.

    Google Scholar 

  • Siffel, P., Vacha, F.: LHC aggregation in intact leaves of tobacco plants stressed by CO2 starvation. — In: Mathis, P. (ed.): Photosynthesis: from Light to Biosphere. Vol. I. Pp. 111–114. Kluwer Academic Publ., Dordrecht-Boston-London 1995.

    Google Scholar 

  • Siffel, P., Vacha, F.: Aggregation of the light-harvesting complex in intact leaves of tobacco plants stressed by CO2 deficit. — Photochem. Photobiol. 67: 304–311, 1998.

    Article  Google Scholar 

  • Siffel, P., Vacha, F.: Pheophytin reduction and fluorescence quenching in reaction centres of Photosystem II.-PS 2001 Proceedings. 12th International Congress on Photosynthesis. S22-034. Pp. 1–4, CSIRO, Collingwood 2001.

    Google Scholar 

  • Siffel, P., Vavrinec, E.: Excitation energy transfer between chlorophylls b and a in polystyrene foils.-Photosynthetica 14: 477–481, 1980.

    Google Scholar 

  • Stys, D., Siffel, P., Hunalova, I., Nebesarova, J.: The relation between changes in non-photochemical quenching, low temperature fluorescence emission, and membrane ultrastructure upon binding of polyionic compounds and fragments of light-harvesting complex 2. — Photosynthetica 37: 325–334, 1999.

    Article  Google Scholar 

  • Synkova, H., Pechova, R., Polanska, L., Husak, M., Siffel, P., Vacha, F., Malbeck, J., Machackova, I., Nebesarova, J.: Three-dimensional reconstructi on of anomalous chloroplasts in transgenic ipt tobacco.-Planta, accepted, 2005a.

  • Synkova, H., Schnablova, R., Husak, M., Siffel, P., Bumba, L., Hunalova, I., Vacha, F.: Isolation and characterization of paracrystalline structures from transgenic Pssu-ipt tobacco. — Photosynthetica 43: accepted, 2005b.

  • Vacha, F., Durchan, M., Siffel, P.: Excitonic interactions in the reaction centre of photosystem II studied by circular dichroism. — PS 2001 Proceedings. 12th International Congress on Photosynthesis. S22-004. Pp. 1–6. CSIRO, Collingwood 2001.

    Google Scholar 

  • Vacha, F., Durchan, M., Siffel, P.: Excitonic interactions in the reaction centre of photosystem II studied by using circular dichroism. — Biochim. biophys. Acta 1554: 147–152, 2002.

    PubMed  Google Scholar 

  • Vacha, F., Kuty, M., Durchan, M., Siffel, P., Psencik, J.: Localisation of accumulated chlorophyll cation in reaction centre of photosystem II. — Mater. Struct. 10: 21–24, 2003.

    Google Scholar 

  • Vacha, F., Psencik, P., Kuty, M., Durchan, M., Siffel, P.: Evidence for localisation of accumulated chlorophyll cation on the D1-accessory chlorophyll in reaction centre of photosystem II. — Photosynth. Res. 83: 279–302, 2005.

    Article  Google Scholar 

  • Zehetner, A., Scheer, H., Siffel, P., Vacha, F.: Photosystem II reaction center with altered pigment-composition: reconstitution of a complex containing five chlorophyll a per two pheophytin a with modified chlorophylls.-Biochim. biophys. Acta 1556: 21–28, 2002.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to J. Kveton.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kveton, J., Durchan, M., Rohacek, K. et al. Pavel Siffel (1954–2003) or Life full of chlorophyll. Photosynthetica 43, 323–328 (2005). https://doi.org/10.1007/s11099-005-0055-5

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11099-005-0055-5

Additional key words

Navigation