Skip to main content
SpringerLink
Log in

Genes differentially expressed under photoinhibition stress in flag leaves of super-hybrid rice Liangyoupeijiu (Oryza sativa) and their genetic origins

  • Published:
Photosynthetica

Abstract

Genes differentially expressed under high irradiance (HI) stress in mature flag leaves of super-hybrid rice Liangyoupeijiu (Oryza sativa ssp. indica) were studied by the silver-staining mRNA differential display technique. We obtained 167 differentially displayed bands on silver-stained polyacrylamide gels and searched for their genetic origins. Five cDNA fragments, which were upregulated by HI stress, were cloned and sequenced. The clones of G25×320, A31×380, and G29×280 putatively encode a cytosolic monodehydroascorbate reductase (MDAR), a phosphatidylinositol 4-kinase (PI-4-K), and a DEAH-box RNA helicase, respectively. Most differentially expressed genes in hybrid rice were inherited from parents and many of them were related with both parents, although some were derived from one parent only. The differentially expressed cDNA fragments having no distinct genetic origins indicated the involvement of some unclear mechanisms in the inheritance processes from parents to hybrid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

A:

antheraxanthin

APX:

ascorbate peroxidases

CAT:

catalases

cysQ:

ammonium transport protein

DDRT-PCR:

differential display reverse transcription polymerase chain reaction

gyrA:

DNA gyrase

HO·:

hydroxyl radical

H2O2 :

hydrogen peroxide

LHC2:

light-harvesting complex of photosystem 2

MDAR:

monodehydroascorbate reductase

1O2 :

singlet oxygen

O2 − :

superoxide radical

ORF:

open reading-frame

PAR:

photosynthetically active radiation

PCR:

polymerase chain reaction

PI:

phosphatidylinositol

PI-4-K:

phosphatidylinositol 4-kinase

PS2:

photosystem 2

RACE:

rapid amplification of cDNA ends

ROS:

reactive oxygen species

RT-PCR:

reverse transcription polymerase chain reaction

SOD:

superoxide dismutases

V:

violaxanthin

Z:

zeaxanthin

Reference

  • Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J.: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. — Nucl. Acids Res. 25: 3389–3402, 1997.

    Article  PubMed  Google Scholar 

  • Arenas, J.E., Abelson, J.N.: Prp43: an RNA helicase-like factor involved in spliceosome disassembly. — Proc. nat. Acad. Sci. USA 94: 11798–11802, 1997.

    Article  PubMed  Google Scholar 

  • Asada, K.: The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons. — Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 601–639, 1999.

    Google Scholar 

  • Bielski, B.H.J.: Chemistry of ascorbic acid radicals. — In: Seib, P.A., Tolbert, B.M. (ed.): Ascorbic Acid, Chemistry, Metabolism and Uses. Pp. 81–100. American Chemical Society, Washington 1982.

    Google Scholar 

  • Bodannes, R.S., Chan, P.C.: Ascorbic acid as a scavenger of singlet oxygen. — FEBS Lett. 105: 195–196, 1979.

    Article  PubMed  Google Scholar 

  • Bohnert, H.J., Sheveleva, E.: Plant stress adaptations — making metabolism move. — Curr. Opin. Plant Biol. 1: 267–274, 1998.

    Article  PubMed  Google Scholar 

  • Chen, H.C., Klein, A., Xiang, M., Backhaus, R.A., Kuntz, M.: Drought-and wound-induced expression in leaves of a gene encoding a chromoplast carotenoid-associated protein. — Plant J. 14: 317–326, 1998.

    Article  Google Scholar 

  • Chomczynski, P., Sacchi, N.: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. — Anal. Biochem. 162: 156–159, 1987.

    PubMed  Google Scholar 

  • Company, M., Arenas, J., Abelson, J.: Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes. — Nature 349: 487–493, 1991.

    Article  PubMed  Google Scholar 

  • Conklin, P.L., Williams, E.H., Last, R.L.: Environmental stress sensitivity of an ascorbic acid-deficient Arabidopsis mutant. — Proc. nat. Acad. Sci. USA 93: 9970–9974, 1996.

    Article  PubMed  Google Scholar 

  • de la Cruz, J., Kressler, D., Linder, P.: Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. — Trends biochem. Sci. 24: 192–198, 1999.

    Article  PubMed  Google Scholar 

  • Demmig-Adams, B., Adams, W.W., III: The role of xanthophyll cycle carotenoids in the protection of photosynthesis. — Trends Plant Sci. 1: 21–26, 1996.

    Article  Google Scholar 

  • Deng, X., Hu, Z.A., Wang, H.X.: mRNA differential display visualized by silver staining tested on gene expression in resurrection plant Boea hygrometrica. — Plant mol. Biol. Rep. 17: 1–7, 1999.

    Article  Google Scholar 

  • Drobak, B.K.: The plant phosphoinositide system. — Biochem. J. 288: 697–712, 1992.

    PubMed  Google Scholar 

  • Dunaeva, M., Adamska, I.: Identification of genes expressed in response to light stress in leaves of Arabidopsis thaliana using RNA differential display. — Eur. J. Biochem. 268: 5521–5529, 2001.

    PubMed  Google Scholar 

  • Gehrmann, T., Heilmeyer, L.M., Jr.: Phosphatidylinositol 4-kinases. — Eur. J. Biochem. 253: 357–370, 1998.

    Article  PubMed  Google Scholar 

  • Horton, P., Ruban, A.V.: Regulation of Photosystem II. — Photosynth. Res. 34: 375–385, 1992.

    Article  Google Scholar 

  • Jagendorf, A.T.: Photophosphorylation and the Hill reaction. — Nature 191: 679–680, 1961.

    Google Scholar 

  • Jiao, D.M., Ji, B.H.: Photoinhibition in indica and japonica subspecies of rice (Oryza sativa) and their reciprocal F1 hybrids. — Aust. J. Plant Physiol. 28: 299–306, 2001.

    Google Scholar 

  • Kok, B.: On the inhibition of photosynthesis by intense light. — Biochim. biophys. Acta 21: 233–244, 1956.

    Google Scholar 

  • Li, F.S., Barnathan, E., Kariko, K.: Rapid method for screening and cloning of cDNA generated in differential mRNA display: application of Northern blot for affinity capturing of cDNA. — Nucl. Acids Res. 22: 1764–1765, 1994.

    PubMed  Google Scholar 

  • Liang, P., Pardee, A.B.: Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. — Science 257: 967–971, 1992.

    PubMed  Google Scholar 

  • Long, S.P., Humphries, S., Falkowski, P.G.: Photoinhibition of photosynthesis in nature. — Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 633–662, 1994.

    Article  Google Scholar 

  • Luking, A., Stahl, U., Schmidt, U.: The protein family of RNA helicases. — Crit. Rev. Biochem. mol. Biol. 33: 259–296, 1998.

    Article  PubMed  Google Scholar 

  • Michael, J.F., Louise, B., Kevin, O., Stanislaw, K., Philip, M.M., Neil, R.B.: Control of ascorbate peroxidase 2 expression by hydrogen peroxide and leaf water status during excess light stress reveals a functional organisation of Arabidopsis leaves. — Plant J. 33: 691–705, 2003.

    Article  PubMed  Google Scholar 

  • Munnik, T., Irvine, R.F., Musgrave, A.: Phospholipid signaling in plants. — Biochim. biophys. Acta 1389: 222–272, 1998.

    PubMed  Google Scholar 

  • Niyogi, K.K.: Photoprotection revised: Genetic and molecular approaches. — Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 333–359, 1999.

    Article  PubMed  Google Scholar 

  • Noam, A., Hagit, Z., Susana, S., Itzhak, O.: Photoinhibition — a historical perspective. — Photosynth. Res. 76: 343–370, 2003.

    Article  Google Scholar 

  • Pfundel, E., Bilger, W.: Regulation and possible function of the violaxanthin cycle. — Photosynth. Res. 42: 89–109, 1994.

    Google Scholar 

  • Powles, S.B.: Photoinhibition of photosynthesis induced by visible light. — Annu. Rev. Plant Physiol. 35: 15–44, 1984.

    Article  Google Scholar 

  • Staley, J., Guthrie, C.: Mechanical gadgets in the spliceosome: clocks, motors, springs and things. — Cell 92: 315–326, 1998.

    PubMed  Google Scholar 

  • Wang, Q., Zhang, Q.D., Zhu, X.G., Lu, C.M., Kuang, T.Y., Li, C.Q.: PSII photochemistry and xanthophylls cycle in two superhigh-yield rice hybrids, Liangyoupeijiu and Hua-an 3 during photoinhibition and subsequent restoration. — Acta bot. sin. 44: 1297–1302, 2002.

    Google Scholar 

  • Whitmarsh, J., Samson, G., Poulson, M.: Photoprotection in photosystem II-the role of cytochrome b559. — In: Baker, N.R., Bowyer, J.R. (ed.): Photoinhibition of Photosynthesis From Molecular Mechanism to the Field. Pp. 75–93. Bios Scientific Publishers, Oxford 1994.

    Google Scholar 

  • Zegzouti, H., Marty, C., Jones, B., Bouquin, T., Latche, A., Pech, J.C., Bouzayen, M.: Improved screening of cDNAs generated by mRNA differential display enables the selection of true positives and the isolation of weekly expressed messages. — Plant mol. Biol. Rep. 15: 236–245, 1997.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory of Photosynthesis and Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, 100093, Beijing, P.R. China

    G.-Q. Jiang, J.-L. Guo, H.-X. Wang, Z.-A. Hu & T.-Y. Kuang

  2. College of Life Sciences, Shandong Agricultural University, Tai’an, 271018, Shandong, P.R. China

    G.-Q. Jiang & Q.-W. Meng

  3. College of Bioengineering, Fujian Normal University, Fuzhou, 350007, Fujian, P.R. China

    J.-L. Guo & Y.-Q. Chen

Authors
  1. G.-Q. Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J.-L. Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Q.-W. Meng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y.-Q. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H.-X. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Z.-A. Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. T.-Y. Kuang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Z.-A. Hu.

Additional information

This research was supported by the State Key Basic Research and Development Plan of China (G1998010100).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jiang, GQ., Guo, JL., Meng, QW. et al. Genes differentially expressed under photoinhibition stress in flag leaves of super-hybrid rice Liangyoupeijiu (Oryza sativa) and their genetic origins. Photosynthetica 43, 231–236 (2005). https://doi.org/10.1007/s11099-005-0038-6

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11099-005-0038-6

Additional key words

Search

Navigation