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Novel Nanostructured Lipid Carriers Co-Loaded with Mesalamine and Curcumin: Formulation, Optimization and In Vitro Evaluation

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Abstract

Purpose

The aim of current study is to formulate, optimize and characterize the developed formulation of Mesalamine-Curcumin Nanostructured Lipid Carriers (Mes-Cur NLCs).

Methods

It was formulated using high pressure homogenization followed by probe sonication and formulation variables were optimized using Central Composite Design. The particle size (PS), zeta potential (ZP), entrapment efficiency (EE), drug release, cytotoxicity on NIH 3T3 fibroblasts cells and HaCaT keratinocytes cells and efficacy on RAW264.7 cells for optimized formulation was determined.

Results

The PS, ZP and EE were found to be 85.26 nm, −23.7 ± 7.45 mV, 99.2 ± 2.62 % (Mes) and 84 ± 1.51 % (Cur), respectively. The good correlation between predicted and obtained value indicated suitability and reproducibility of experimental design. NLCs showed spherical shape as confirmed by TEM. In vitro drug release profile of prepared formulation showed that Mes exhibited 100 % release at 48 h, whereas Cur exhibited 82.23 ± 2.97% release at 120 h. Both the drugs exhibited sustained release upon incorporation into the NLCs. The absence of any significant cell death during MTT assay performed on NIH 3T3 fibroblasts cells and HaCaT keratinocytes cells indicated that NLCs’ were safe for use. Furthermore, significant reduction in nitric oxide level during anti-inflammatory evaluation of formulation on RAW264.7 cells showed excellent potential for the formulation to treat inflammation. The formulation was found stable as no significant difference between the PS, ZP and EE of the fresh and aged NLCs was observed.

Conclusion

The outcomes of study deciphered successful formulation of Mes-Cur NLCs.

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Data Availability

Not Applicable.

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Acknowledgements

Authors are thankful to Central Instrumentation Facility, Lovely Professional University for providing necessary support in terms of sample analysis using sophisticated instruments such as SEM and HPLC.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Author information

Authors and Affiliations

  1. School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, 144411, India

    Ankit Awasthi, Bimlesh Kumar, Monica Gulati, Sukriti Vishwas, Leander Corrie, Jaskiran Kaur, Rubiya Khursheed, Malakapogu Ravindra Babu, M. V. N. L. Chaitanya, Ankit Kumar, Narendra Kumar Pandey & Sachin Kumar Singh

  2. Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, NSW, 2007, Australia

    Monica Gulati, Kamal Dua & Sachin Kumar Singh

  3. Department of Pharmacognosy, Faculty of Pharmacy, Tishk International University-44001, Erbil, Kurdistan Region, Iraq

    Rozhan Arif Muhammed, Duran Kala & Omji Porwal

  4. Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana, 124001, India

    Harish Dureja

  5. Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil, 57000, Kuala Lumpur, Malaysia

    Dinesh Kumar Chellappan

  6. Department of Biotechnology, School of Engineering & Technology (SET), Sharda University, Plot No.32-34 Knowledge Park III, Greater Noida, Uttar Pradesh, 201310, India

    Niraj Kumar Jha

  7. School of Pharmacy, Suresh Gyan Vihar University, Mahal Road, Jagatpura, Jaipur, India

    Gaurav Gupta

  8. Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India

    Gaurav Gupta

  9. Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India

    Gaurav Gupta

  10. Department of Chemistry, University of Petroleum & Energy Studies, Energy Acres, Dehradun, 248007, India

    Parteek Prasher

  11. Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Shoolini University, Solan, 173229, India

    Deepak Kumar

  12. Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, Ultimo, NSW, 2007, Australia

    Kamal Dua

Authors
  1. Ankit Awasthi
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  2. Bimlesh Kumar
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  3. Monica Gulati
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  5. Leander Corrie
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  6. Jaskiran Kaur
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  7. Rubiya Khursheed
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  9. Duran Kala
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  15. Harish Dureja
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  20. Deepak Kumar
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  21. Kamal Dua
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Contributions

Authors’ Contributions

S.N.

Authors

CRediT role(s)

1

Ankit Awasthi

Methodology, Data Curation and Writing – original draft

2

Bimlesh Kumar

Supervision

3

Monica Gulati

Supervision

4

Sukriti Vishwas

Methodology

5

Leander Corrie

Methodology

6

Jaskiran Kaur

Methodology

7

Rubiya Khursheed

Methodology

8

Rozhan Arif Muhammed

Methodology

9

Duran Kala

Methodology

10

Omji Porwal

Methodology

11

Malakapogu Ravindra Babu

Methodology

12

M.V.N.L. Chaitanya

Methodology

13

Ankit Kumar

Methodology

14

Narendra Kumar Pandey

Methodology

15

Harish Dureja,

Review & editing

16

Dinesh Kumar Chellappan

Review & editing

17

Niraj Kumar Jha

Methodology

18

Gaurav Gupta

Methodology

19

Parteek Prasher

Methodology

20

Deepak Kumar

Methodology

21

Kamal Dua

Supervision

22

Sachin Kumar Singh

Conceptualization, Validation, Supervision, Review & editing

Corresponding author

Correspondence to Sachin Kumar Singh.

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ESM 1

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Awasthi, A., Kumar, B., Gulati, M. et al. Novel Nanostructured Lipid Carriers Co-Loaded with Mesalamine and Curcumin: Formulation, Optimization and In Vitro Evaluation. Pharm Res 39, 2817–2829 (2022). https://doi.org/10.1007/s11095-022-03401-z

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