Pharmaceutical Research

, Volume 34, Issue 11, pp 2241–2249 | Cite as

BITC and S-Carvone Restrain High-Fat Diet-Induced Obesity and Ameliorate Hepatic Steatosis and Insulin Resistance

  • Sary Alsanea
  • Dexi LiuEmail author
Research Paper



To investigate the preventative activity of benzyl isothiocyante and S-carvone against high-fat diet-induced obesity and metabolic complications.


Ten-week-old C57BL/6 male mice were fed a high-fat diet and injected intraperitoneally twice per week with benzyl isothiocyante, S-carvone, or vehicle for 8 weeks. The body weight, food intake, and body composition were monitored, and glucose tolerance and insulin tolerance tests were performed at the end of the experiment. Serum and tissue samples were studied using serum biochemistry, histological, and gene expression analysis to define the effects of benzyl isothiocyante and S-carvone treatments on lipid and glucose metabolism and inflammatory responses.


Benzyl isothiocyante and S-carvone blocked high-fat diet-induced weight gain, fat accumulation in the liver, and insulin resistance. The beneficial effects were found to be associated with an improvement of expression of macrophage marker genes in white adipose tissue, including F4/80, Cd11b, Cd11c, Cd206, and Tnf-α, and reduced expression of genes (Pparγ2, Scd1, Cd36) responsible for lipid synthesis and transport in the liver.


Benzyl isothiocyante and S-carvone block high-fat diet-induced obesity and metabolism disorders and can be considered for management of the obesity epidemic that affects approximately 36% of adults and 17% of children in the USA.

Key words

Obesity Inflammation BITC S-carvone Antioxidant 



Acetyl-CoA carboxylase


Area under the curve


Brown adipose tissue


Benzyl isothiocyanate


Dimethyl sulfoxide


Epididymal white adipose tissues


Fatty acid synthase


Free fatty acid


Glucose tolerance test


High-fat diet


Homeostatic model assessment of insulin resistance


Insulin tolerance test


Inguinal white adipose tissues


Peroxisome proliferator-activated receptor-γ


Perirenal white adipose tissues


Stearoyl-CoA desaturase


Sterol regulatory element-binding protein




Tumor necrosis factor α


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.Department of Pharmaceutical and Biomedical Sciences, College of PharmacyUniversity of GeorgiaAthensUSA

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