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Pharmaceutical Research

, Volume 34, Issue 11, pp 2241–2249 | Cite as

BITC and S-Carvone Restrain High-Fat Diet-Induced Obesity and Ameliorate Hepatic Steatosis and Insulin Resistance

  • Sary Alsanea
  • Dexi LiuEmail author
Research Paper

Abstract

Purpose

To investigate the preventative activity of benzyl isothiocyante and S-carvone against high-fat diet-induced obesity and metabolic complications.

Methods

Ten-week-old C57BL/6 male mice were fed a high-fat diet and injected intraperitoneally twice per week with benzyl isothiocyante, S-carvone, or vehicle for 8 weeks. The body weight, food intake, and body composition were monitored, and glucose tolerance and insulin tolerance tests were performed at the end of the experiment. Serum and tissue samples were studied using serum biochemistry, histological, and gene expression analysis to define the effects of benzyl isothiocyante and S-carvone treatments on lipid and glucose metabolism and inflammatory responses.

Results

Benzyl isothiocyante and S-carvone blocked high-fat diet-induced weight gain, fat accumulation in the liver, and insulin resistance. The beneficial effects were found to be associated with an improvement of expression of macrophage marker genes in white adipose tissue, including F4/80, Cd11b, Cd11c, Cd206, and Tnf-α, and reduced expression of genes (Pparγ2, Scd1, Cd36) responsible for lipid synthesis and transport in the liver.

Conclusion

Benzyl isothiocyante and S-carvone block high-fat diet-induced obesity and metabolism disorders and can be considered for management of the obesity epidemic that affects approximately 36% of adults and 17% of children in the USA.

Key words

Obesity Inflammation BITC S-carvone Antioxidant 

Abbreviations

ACC

Acetyl-CoA carboxylase

AUC

Area under the curve

BAT

Brown adipose tissue

BITC

Benzyl isothiocyanate

DMSO

Dimethyl sulfoxide

eWAT

Epididymal white adipose tissues

FAS

Fatty acid synthase

FFA

Free fatty acid

GTT

Glucose tolerance test

HFD

High-fat diet

HOMA-IR

Homeostatic model assessment of insulin resistance

ITT

Insulin tolerance test

iWAT

Inguinal white adipose tissues

PPARγ

Peroxisome proliferator-activated receptor-γ

pWAT

Perirenal white adipose tissues

SCD

Stearoyl-CoA desaturase

SREBP

Sterol regulatory element-binding protein

TG

Triglyceride

TNF-α

Tumor necrosis factor α

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.Department of Pharmaceutical and Biomedical Sciences, College of PharmacyUniversity of GeorgiaAthensUSA

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