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A Functional Iron Oxide Nanoparticles Modified with PLA-PEG-DG as Tumor-Targeted MRI Contrast Agent

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Abstract

Purpose

Tumor targeting could greatly promote the performance of magnetic nanomaterials as MRI (Magnetic Resonance Imaging) agent for tumor diagnosis. Herein, we reported a novel magnetic nanoparticle modified with PLA (poly lactic acid)-PEG (polyethylene glycol)-DG (D-glucosamine) as Tumor-targeted MRI Contrast Agent.

Methods

In this work, we took use of the D-glucose passive targeting on tumor cells, combining it on PLA-PEG through amide reaction, and then wrapped the PLA-PEG-DG up to the Fe3O4@OA NPs. The stability and anti phagocytosis of Fe3O4@OA@PLA-PEG-DG was tested in vitro; the MRI efficiency and toxicity was also detected in vivo.

Results

These functional magnetic nanoparticles demonstrated good biocompatibility and stability both in vitro and in vivo. Cell experiments showed that Fe3O4@OA@PLA-PEG-DG nanoparticles exist good anti phagocytosis and high targetability. In vivo MRI images showed that the contrast effect of Fe3O4@OA@PLA-PEG-DG nanoparticles prevailed over the commercial non tumor-targeting magnetic nanomaterials MRI agent at a relatively low dose.

Conclusions

The DG can validly enhance the tumor-targetting effect of Fe3O4@OA@PLA-PEG nanoparticle. Maybe MRI agents with DG can hold promise as tumor-targetting development in the future.

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Abbreviations

18F–FDG:

[18F] fluoro-2-deoxy-D-glucose

DG:

D-glucosamine

GLUT1:

Glucose transporters 1

NPs:

Nanoparticles

PEG:

Polyethylene glycol

PLA:

Poly lactic acid

SPIONs:

Superparamagnetic iron oxide nanoparticles

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Authors and Affiliations

  1. State Key Laboratory of Bioelectronics, Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China

    Fei Xiong, Ke Hu, Haoli Yu, Lina Song, Yu Zhang & Ning Gu

  2. Collaborative Innovation Center of Suzhou Nano-Science and Technology, Suzhou Key Laboratory of Biomaterials and Technologies, Suzhou, 215123, China

    Fei Xiong, Ke Hu, Haoli Yu, Lina Song, Yu Zhang & Ning Gu

  3. Institute of Pharmaceutics, China Pharmaceutical University, Nanjing, 210096, China

    Lijun Zhou

  4. Department of Radiology The Affiliated Renmin Hospital, Jiangsu University, Zhenjiang, China

    Xiuhong Shan & Jianping Liu

Authors
  1. Fei Xiong
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  2. Ke Hu
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  3. Haoli Yu
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  6. Yu Zhang
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  7. Xiuhong Shan
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  9. Ning Gu
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Correspondence to Fei Xiong or Ning Gu.

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Xiong, F., Hu, K., Yu, H. et al. A Functional Iron Oxide Nanoparticles Modified with PLA-PEG-DG as Tumor-Targeted MRI Contrast Agent. Pharm Res 34, 1683–1692 (2017). https://doi.org/10.1007/s11095-017-2165-8

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  • DOI: https://doi.org/10.1007/s11095-017-2165-8

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