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Effect of Nanoparticle Surface on the HPLC Elution Profile of Liposomal Nanoparticles

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Abstract

Purpose

Nanoparticles have been used in diverse areas, and even broader applications are expected in the future. Since surface modification can influence the configuration and toxicity of nanoparticles, a rapid screening method is important to ensure nanoparticle quality.

Methods

We examined the effect of the nanoparticle surface morphology on the HPLC elution profile using two types of 100-nm liposomal nanoparticles (AmBisomeⓇ and DOXILⓇ).

Results

These 100-nm-sized nanoparticles eluted before the holdup time (about 4 min), even when a column packed with particles with a relatively large pore size (30 nm) was used. The elution time of the nanoparticles increased with pegylation of the nanoparticles and protein adsorption to the nanoparticles; however, the nanoparticles still eluted before the holdup time.

Conclusions

The results of this study indicate that HPLC is a suitable tool for rapid evaluation of the surface of liposomal nanoparticles.

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Abbreviations

BCA:

Bicinchoninic acid

CE:

Capillary electrophoresis

DAD:

Diode array detector

DLS:

Dynamic light scattering

Ex.:

Excitation

Em.:

Emission

DSPE:

1,2-Distearoyl-sn-glycero-3-phosphoethanolamine

HPLC:

High performance liquid chromatography

NMR:

Nuclear magnetic resonance

PEG:

Polyethyleneglycol

TSP:

3-(Trimethylsilyl)propionic 2,2,3,3-d4 acid

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ACKNOWLEDGMENTS AND DISCLOSURES

This work was supported by grants (Kakenhi) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan, and JSPS Core-to-Core Program, A. Advanced Research Networks. We thank Prof. T. Ozeki (Nogoya City Univ., Japan) and Mr. K. Kurono (Shoko Scientific Co., Ltd., Japan) for advice of pegylation of AmBisomeⓇ and technical assistance with the size analysis of the nanoparticles using MALS, respectively. The author declares no competing financial interest.

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan

    Naoki Itoh, Tomofumi Santa, Takashi Funatsu & Masaru Kato

  2. DDS Research, Global Formulation Research Japan, Pharmaceutical Science and Technology, Core Function Unit, Eisai Product Creation Systems, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki, 300-2635, Japan

    Eiichi Yamamoto

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  1. Naoki Itoh
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Correspondence to Masaru Kato.

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Itoh, N., Yamamoto, E., Santa, T. et al. Effect of Nanoparticle Surface on the HPLC Elution Profile of Liposomal Nanoparticles. Pharm Res 33, 1440–1446 (2016). https://doi.org/10.1007/s11095-016-1886-4

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  • DOI: https://doi.org/10.1007/s11095-016-1886-4

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