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Pharmaceutical Research

, Volume 32, Issue 3, pp 1094–1104 | Cite as

Could Albumin Affect the Self-Assembling Properties of a Block Co-polymer System and Drug Release? An In-Vitro Study

  • Diego R. Perinelli
  • Giulia Bonacucina
  • Stefania Pucciarelli
  • Marco Cespi
  • Luca Casettari
  • Valeria Polzonetti
  • Francesco Martino Carpi
  • Giovanni F. Palmieri
Research Paper

ABSTRACT

Purpose

This work investigated the influence of a model protein, bovine serum albumin (BSA), on the properties of a thermogelling formulation intended for administration inside body compartments where there is high albumin content, as in the case of inflamed joints; it also explored the relation between the variation of these properties and release performance of methotrexate (MTX), a drug used to treat forms of arthritis and rheumatic conditions.

Methods

The influence of BSA on the micellisation and gelation behaviour of Poloxamer 407, chosen as a model copolymer, was studied by differential scanning calorimetry (microDSC), dynamic light scattering (DLS), fluorescence spectroscopy and rheology studies. A release study of MTX loaded inside the hydrogel in presence and in absence of BSA was performed.

Results

DLS and microDSC data revealed that the micellisation process was not affected by the protein, as demonstrated by unaltered micellar size and thermodynamic parameters. While the presence of BSA in the copolymer system reduced gel consistency, the hydrogel release performance was only slightly affected.

Conclusion

Our results suggested that the kinetics of MTX release mainly depended on the presence of the thermogelling copolymer, although other mechanisms related to BSA could be involved. Finally, the study assessed the feasibility of using a thermogelling hydrogel for in situ drug administration in areas with the presence of high protein concentrations.

KEY WORDS

BSA methotrexate poloxamer 407 polymer-protein interaction sustained release 

Supplementary material

11095_2014_1521_MOESM1_ESM.doc (34 kb)
Table ST1 (DOC 34 kb)
11095_2014_1521_MOESM2_ESM.doc (28 kb)
Table ST2 (DOC 27 kb)
11095_2014_1521_MOESM3_ESM.doc (29 kb)
Table ST3 (DOC 29 kb)
11095_2014_1521_Fig8_ESM.jpg (625 kb)
Figure SF 1

Effect of the increasing concentration of P407 on the intrinsic fluorescence emission of BSA at 35°C. (JPEG 625 kb)

11095_2014_1521_Fig9_ESM.jpg (662 kb)
Figure SF 2

Effect of the increasing concentration of P407 on the intrinsic fluorescence emission of BSA at 10°C. (JPEG 662 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Diego R. Perinelli
    • 1
  • Giulia Bonacucina
    • 1
  • Stefania Pucciarelli
    • 2
  • Marco Cespi
    • 1
  • Luca Casettari
    • 3
  • Valeria Polzonetti
    • 2
  • Francesco Martino Carpi
    • 2
  • Giovanni F. Palmieri
    • 1
  1. 1.School of PharmacyUniversity of CamerinoCamerinoItaly
  2. 2.School of Biosciences and BiotechnologyUniversity of CamerinoCamerinoItaly
  3. 3.Department of Biomolecular SciencesUniversity of UrbinoUrbinoItaly

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