Characterization of a Nanoparticulate Drug Delivery System Using Scanning Ion Occlusion Sensing
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To explore the application of scanning ion occlusion sensing (SIOS) as a novel technology for characterization of nanoparticles.
Liposomes were employed as model nanoparticles. The size distribution of the liposomes was measured by both SIOS and dynamic light scattering (DLS). Particle number concentration was determined based on particle translocation rate. The ability of SIOS and DLS to resolve bimodal samples was evaluated by measuring a mixture of 217 and 355 nm standard nanoparticles. Opsonization of liposomes by plasma was also studied using SIOS.
SIOS was shown to measure the size of different liposomes with higher sensitivity than DLS and it requires a smaller sample volume than DLS. With appropriate calibration, SIOS could be used to determine particle number concentrations. In comparison, SIOS analysis of the mixture showed accurate resolution of the population as a bimodal distribution over a wide range of number ratios of the particles. SIOS could detect plasma opsonization of liposomes by demonstrating a increase in particle size and also changes in the particle translocation rate.
SIOS is a useful technology for nanoparticle characterization. It shows some advantages over DLS and is clearly a useful tool for the study of nanoparticle drug delivery systems.
KEY WORDSDLS liposomes nanoparticles opsonization SIOS
ACKNOWLEDGMENTS AND DISCLOSURES
The research was conducted during the tenure of a Health Sciences Career Development Award from the University of Otago to Lin Yang. The authors report no conflicts of interest.
- 3.Husain A, Hamielec AE, Vlachopoulos J. Particle size analysis using size exclusion chromatography. Size exclusion chromatography (GPC). Am Chem Soc. 1980;138:47–75.Google Scholar
- 10.Willmott GR, Vogel R, Yu SSC, Groenewegen LG, Roberts GS, Kozak D, et al. Use of tunable nanopore blockade rates to investigate colloidal dispersions. J Phys.: Condens Matter. 2010;22:454116.Google Scholar
- 11.Vogel R, Willmott G, Kozak D, Roberts GS, Anderson W, Groenewegen L, et al. Quantitative sizing of nano/microparticles with a tunable elastomeric pore sensor. Anal Chem. 2011;83:3499–3506.Google Scholar
- 18.Roberts GS, Yu S, Zeng Q, Chan LCL, Anderson W, Colby AH, et al. Tunable pores for measuring concentrations of synthetic and biological nanoparticle dispersions. Biosens Bioelectron. 2012;31:17–25.Google Scholar
- 20.Tscharnuter W. Photon correlation spectroscopy in particle sizing. In: Meyers RA, editor. Encyclopedia of analytical chemistry. Chichester: John Wiley & Sons Ltd; 2000. p. 5469–85.Google Scholar