ABSTRACT
Purpose
To develop and evaluate liposomal formulations prepared with an isolated mitochondrial fraction as a mitochondria-specific delivery vehicle
Methods
Liposomes were prepared with either a crude mitochondrial fraction extracted from cells or lipids extracted from the crude mitochondrial fraction and were then characterized by determining their size and zeta potential. The cell uptake of the liposomes and loaded bioactive was studied using flow cytometry and confocal microscopy. The cytotoxicity of the formulations was tested by MTS cytotoxicity assay.
Results
Liposomes prepared with the mitochondrial extracts were non-toxic and colocalized with mitochondria in F98 cells. Addition of DOTAP to the liposomes facilitated DNA complexation and the DNA delivered intracellularly co-localized with mitochondria.
Conclusion
The results from this study establish the potential of using a mitochondrial fraction isolated from cells to prepare liposomes capable of delivering biologically active molecules to mitochondria of live mammalian cells.
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ACKNOWLEDGMENTS & DISCLOSURES
The authors wish to thank Prof. Vladimir P. Torchilin at Northeastern University, Boston, MA, for generous access to the flow cytometer and zetasizer. This work was supported in part by a faculty development grant from the MCPHS faculty development committee to GGMD and a MCPHS summer undergraduate research fellowship award to LEM.
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Wagle, M.A., Martinville, L.E. & D’Souza, G.G.M. The Utility of an Isolated Mitochondrial Fraction in the Preparation of Liposomes for the Specific Delivery of Bioactives to Mitochondria in Live Mammalian Cells. Pharm Res 28, 2790–2796 (2011). https://doi.org/10.1007/s11095-011-0531-5
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DOI: https://doi.org/10.1007/s11095-011-0531-5