ABSTRACT
Purpose
To prepare mesoporous silica-based delivery systems capable of simultaneous delivery of drugs and nucleic acids.
Methods
The surface of mesoporous silica nanoparticles (MSN) was modified with poly(ethylene glycol) (PEG) and poly(2-(dimethylamino)ethylmethacrylate) (PDMAEMA) or poly(2-(diethylamino)ethylmethacrylate) (PDEAEMA). The particles were then loaded with a lysosomotropic agent chloroquine (CQ) and complexed with plasmid DNA or siRNA. The ability of the synthesized particles to deliver combinations of CQ and nucleic acids was evaluated using luciferase plasmid DNA and siRNA targeting luciferase and GAPDH.
Results
The results show a slow partial MSN dissolution to form hollow silica nanoparticles in aqueous solution. The biological studies show that polycation-modified MSN are able to simultaneously deliver CQ with DNA and siRNA. The co-delivery of CQ and the nucleic acids leads to a significantly increased transfection and silencing activity of the complexes compared with MSN not loaded with CQ.
Conclusion
PEGylated MSN modified with polycations are promising delivery vectors for combination drug/nucleic acid therapies.
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Abbreviations
- APTES:
-
3-aminopropyltriethoxysilane
- CQ:
-
chloroquine
- CTAB:
-
N-cetyltrimethylammonium bromide
- DMEM:
-
Dulbecco’s modified Eagle’s medium
- GAPDH:
-
glyceraldehyde 3-phosphate dehydrogenase
- MPA:
-
3-mercaptopropionic acid
- MPTMS:
-
3-mercaptopropyltrimethoxysilane
- MSN:
-
mesoporous silica nanoparticles
- PDEAEMA:
-
poly(2-(diethylamino)ethylmethacrylate)
- PEG:
-
poly(ethylene glycol)
- PEI:
-
polyethyleneimine
- PDMAEMA:
-
poly(2-(dimethylamino)ethylmethacrylate)
- PMSN:
-
PEG-coated MSN
- TEOS:
-
tetraethylorthosilicate
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ACKNOWLEDGMENTS
This work was supported by NIH Grant EB0043588 from the National Institute of Biomedical Imaging and Bioengineering.
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Bhattarai, S.R., Muthuswamy, E., Wani, A. et al. Enhanced Gene and siRNA Delivery by Polycation-Modified Mesoporous Silica Nanoparticles Loaded with Chloroquine. Pharm Res 27, 2556–2568 (2010). https://doi.org/10.1007/s11095-010-0245-0
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DOI: https://doi.org/10.1007/s11095-010-0245-0